GARS (1C6) Monoclonal Antibody

$Lane 1: Jurkat Cell lysates; Lane 2: Ramos Cell lysates; Lane 3: HT-1080 Cell lysates; probed with GARS (1C6) Monoclonal Antibody, unconjugated (bsm-51350M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Jurkat Cell lysates; Lane 2: Ramos Cell lysates; Lane 3: HT-1080 Cell lysates; probed with GARS (1C6) Monoclonal Antibody, unconjugated (bsm-51350M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells; antibody incubation with GARS (1C6) Monoclonal Antibody, Unconjugated (bsm-51350M) at 1:25 overnight at 4\u00b0C, followed by a conjugated secondary (green). Cytoplasmic actin is detected with Dylight\u00ae 554 Phalloidin at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).$

Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells; antibody incubation with GARS (1C6) Monoclonal Antibody, Unconjugated (bsm-51350M) at 1:25 overnight at 4\u00b0C, followed by a conjugated secondary (green). Cytoplasmic actin is detected with Dylight\u00ae 554 Phalloidin at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).

$Hela cells probed with using GARS (1C6) Monoclonal Antibody (green) unconjugated bsm-51350M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:200 compared to an isotype control of mouse IgG1 (blue).$

Hela cells probed with using GARS (1C6) Monoclonal Antibody (green) unconjugated bsm-51350M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:200 compared to an isotype control of mouse IgG1 (blue).

Applications

Reactivity

Human

Overview
Catalog #	bsm-51350M
Product Name	GARS (1C6) Monoclonal Antibody
Applications	WB FCM IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This GARS antibody is generated from a mouse immunized with a recombinant protein between 15-305 amino acids from human GARS.
Clonality	Monoclonal
Clone #	1C6
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	2617
Swiss Prot	P41250
Synonyms	HMN5; CMT2D; DSMAV; GlyRS; SMAD1; Glycine--tRNA ligase; Diadenosine tetraphosphate synthetase; AP-4-A synthetase; Glycyl-tRNA synthetase; GARS
Background	Catalyzes the attachment of glycine to tRNA(Gly). Is also able produce diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IF