CAMK2 beta Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CAMK2 beta Monoclonal Antibody, Unconjugated (bsm-51658M) at 1:25 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CAMK2 beta Monoclonal Antibody, Unconjugated (bsm-51658M) at 1:25 overnight at 4\u00b0C, DAB staining.

Applications

WB
IHC-P
IHC-F
IF

Predicted Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-51658m
Product Name	CAMK2 beta Monoclonal Antibody
Applications	WB IHC-P IHC-F IF
Predicted Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human CAMK2 between 1-503 amino acids.
Clonality	Monoclonal
Clone #	F4A2
Isotype	IgG1
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	816
Swiss Prot	Q13554
Subcellular location	Cytoplasm, Cell membrane
Synonyms	Calcium/calmodulin dependent protein kinase (CaM kinase) II beta; Calcium/calmodulin dependent protein kinase II beta; Calcium/calmodulin dependent protein kinase IIB; Calcium/calmodulin dependent protein kinase type II beta chain; calcium/calmodulin-dependent protein kinase II beta; Calcium/calmodulin-dependent protein kinase type II subunit beta; CAM 2; CaM kinase II beta chain; CaM kinase II beta subunit; CaM kinase II subunit beta; CaM-kinase II beta chain; CAM2; CAMK 2; CAMK 2B; CaMK II beta subunit; CaMK II subunit beta; CaMK-II subunit beta; CAMK2; Camk2b; CAMKB; CaMKII beta subunit; CaMKIIB; KCC2B_HUMAN; MGC29528; Proline rich calmodulin dependent protein kinase; proline rich calmodulin-dependent protein kinase;
Background	Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in sarcoplamic reticulum Ca(2+) transport in skeletal muscle and may function in dendritic spine and synapse formation and neuronal plasticity. In slow-twitch muscles, is involved in regulation of sarcoplamic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of the ryanodine receptor-coupling factor triadin. In neurons, may participate in the promotion of dendritic spine and synapse formation and maintenance of synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning.
Application Dilution
WB	=1:500-2000
IHC-P	IHC-P=1:25
IHC-F	IHC-F=1:25
IF	IF=1:25