Hexokinase II Monoclonal Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with Hexokinase II monoclonal antibody, unconjugated (bsm-51659M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with Hexokinase II monoclonal antibody, unconjugated (bsm-51659M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
IHC-P
IHC-F
IF

Predicted Reactivity

Human

Overview
Catalog #	bsm-51659m
Product Name	Hexokinase II Monoclonal Antibody
Applications	WB IHC-P IHC-F IF
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human HK2 (Hexokinase II) between 1-170 amino acids.
Clonality	Monoclonal
Clone #	S2G6
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3099
Swiss Prot	P52789
Subcellular location	Cytoplasm, Cell membrane
Synonyms	Hexokinase II; Hexokinase 2 muscle; Hexokinase type II; Hexokinase-2; HK 2; HK II; HK2; HKII; HxK 2; HxK2; HXK2_HUMAN; Muscle form hexokinase; DKFZp686M1669.
Background	Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle.
Application Dilution
WB	=1:500-1000
IHC-P	IHC-P=1:100-500
IHC-F	IHC-F=1:100-400
IF	IF=1:25