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Western Blot Antibodies



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Catalog #1: bs-25282R

Applications: WB

Reactivity: Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PLVAP Polyclonal Antibody, Unconjugated (bs-12737R) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Prostate tissue lysates; Lane 2: Mouse Large intestine tissue lysates probed with PLVAP Polyclonal Antibody, Unconjugated (bs-12737R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-12737R

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Antigen: bs-0719P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-CEA Polyclonal Antibody, Unconjugated (bs-0719R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
MCF7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CEAM5 Antibody(bs-0719R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Human rectal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CEAM5 Polyclonal Antibody, Unconjugated (bs-0719R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0719R

Applications: WB, ELISA, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-13170R

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0295p-bf750-100ul

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor-750

Catalog #1: bs-0295p-bf405-100ul

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor-405

Catalog #1: bs-14241R-BF488

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bsm-43534m-pe-cy7-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Rat

Conjugate: PE-Cy7

Catalog #1: bsm-43017m-biotin-100ul

Applications: WB

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-60709r-bf594-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-594

Tissue: Human breast cancer\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60700R
Tissue: Human colon cancer\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60700R
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PTP1B Monoclonal Antibody, Unconjugated(bsm-60700R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PTP1B Monoclonal Antibody, Unconjugated(bsm-60700R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

Catalog #1: bsm-60700r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-21617R-PE-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bs-24335R-PE

Applications: WB

Reactivity: Human

Conjugate: PE

Catalog #1: bs-10645R-APC-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bsm-33185M-BF350

Applications: WB

Reactivity: Species independent

Conjugate: AbBy Fluor-350

Catalog #1: bs-24331R-BF488

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bs-21310R-PE

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: PE

Catalog #1: bsm-33207M-BF405

Applications: WB

Reactivity: Rat

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33085m-bf488-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33062M-PE-Cy7

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bs-23526R-BF647

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bs-23258R-PE-Cy7

Applications: WB

Reactivity: Human, Rat

Conjugate: PE-Cy7

Catalog #1: bs-23175R-BF647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-647

Catalog #1: bs-20160R-PerCP

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bs-20747R-APC

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: APC

Catalog #1: bs-20373R-APC-Cy5.5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bs-9184R-BF594

Applications: WB, IF(IHC-P)

Reactivity: Mouse

Conjugate: AbBy Fluor-594

Catalog #1: bs-3220R-BF647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-647

Catalog #1: bs-3142R-APC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: APC

Catalog #1: bs-3142R-APC-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: APC-Cy7

Catalog #1: bs-2518R-BF680

Applications: WB, FCM

Reactivity: Mouse

Conjugate: AbBy Fluor-680

Catalog #1: bs-2389R-APC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: APC

Catalog #1: bs-1714R-BF750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: AbBy Fluor-750

Catalog #1: bs-1554R-BF488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bs-1554R-PerCP

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: PerCP

Catalog #1: bs-1554R-APC-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bs-1554R-PE-Cy7

Applications: WB, FCM

Reactivity: Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bs-15533R-PE-Cy7

Applications: WB, FCM

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bs-1513R-BF594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bs-1513R-APC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC

Catalog #1: bs-13681R-BF555

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-555

Catalog #1: bs-13623R-APC-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: APC-Cy7

Catalog #1: bs-13549R-BF488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bs-13536R-PE-Cy7

Applications: WB, FCM

Reactivity: Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bs-12196R-BF488

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor-488

Catalog #1: bs-11936R-BF647

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor-647

Catalog #1: bs-1189R-BF488

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: AbBy Fluor-488

Catalog #1: bs-11884R-BF647

Applications: WB

Reactivity:

Conjugate: AbBy Fluor-647