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Western Blot Antibodies



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Catalog #1: bs-10900R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: HRP

Catalog #1: bs-10802R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bsm-51096M

Applications: WB, IF(ICC), IF

Reactivity:

Conjugate: Unconjugated

Lane 1: HeLa; Lane 2: HCT116 lysate at 20 \u00b5g per lane. probed with bsm-51077M RAB8A (261CT1.3.1) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Lane 1: Mouse brain lysates; probed with RAB8A (261CT1.3.1) Monoclonal Antibody, unconjugated (bsm-51077M) at 1:100 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: U937 cell lysates probed with RAB8A (8A4) Monoclonal Antibody, Unconjugated (bsm-51077M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates probed with RAB8A (8A4) Monoclonal Antibody, Unconjugated (bsm-51077M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51077M

Applications: WB, ELISA

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (RPS6 (9F2)) Monoclonal Antibody, Unconjugated (bsm-51406M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.\r\n\r\n
Paraformaldehyde-fixed, paraffin embedded (human cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (RPS6 (9F2)) Monoclonal Antibody, Unconjugated (bsm-51406M) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.\r\n\r\n
25 ug total protein per lane of various lysates (see on figure) probed with RPS6 (N-term) monoclonal antibody, unconjugated (bsm-51406M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPS6 (N-term) Monoclonal Antibody, Unconjugated (bsm-51406M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-51406m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: MCF-7; Lane 2: Jurkat; Lane 3: PC-12 probed with TOP1 (1C6) Monoclonal Antibody, unconjugated (bsm-51396M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Hela cells stained with TOP1 (1C6) Monoclonal Antibody, bsm-51396M at 1:25 dilution, followed by Alexa Fluor\u00ae 488 goat anti-mouse lgG (green), compared to mouse IgG1 isotype control (blue).
Lane 1: Molt-4 cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: Siha cell lysates; Lane 4: K562 cell lysates probed with TOP1 (1C6) Monoclonal Antibody, Unconjugated (bsm-51396M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51396M

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51378M

Applications: WB, ELISA, Flow-Cyt

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells; Antibody incubation with S100A2 (2C1) Monoclonal Antibody, Unconjugated (bsm-51330M) at 1:25 overnight at 4\u00b0C, followed by a conjugated secondary (green). Cytoplasmic actin is detected with Dylight\u00ae 554 Phalloidin at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with S100A2 Monoclonal Antibody, Unconjugated (bsm-51330M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with S100A2 Monoclonal Antibody, Unconjugated (bsm-51330M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with S100A2 Monoclonal Antibody, Unconjugated (bsm-51330M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-51330m

Applications: IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: SW480 Cell lysates; probed with TIMP2 (4C7) Monoclonal Antibody, unconjugated (bsm-51320M) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 cells labeled with TIMP2 (4C7) Monoclonal Antibody (bsm-51320M) at 1\/25 dilution followed by secondary antibody (green), cytoplasmic actin staining (red), and nuclear counter stain with DAPI (blue).
K562 cells probed with using TIMP2 (4C7) Monoclonal Antibody bsm-51320M at 1:25 for 30 minutes followed by A488 secondary antibody (green) at 1:200, compared to an isotype control of mouse IgG1 (blue).
Paraformaldehyde-fixed, paraffin embedded Human kidney tissue; Blocked with 3% BSA for 30min at RT; Antibody incubation with TIMP2 (4C7) Monoclonal Antibody, Unconjugated (bsm-51320M ) at 1:25 for 1hr at 37\u00b0C, followed by a conjugated secondary antibody and DAB staining.

Catalog #1: bsm-51320M

Applications: WB, FCM, IHC-P, IF(ICC), IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: MCF-7 Cell lysates; Lane 2: U266 Cell lysates; probed with CDKN1B (10B7) Monoclonal Antibody, unconjugated (bsm-51281M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human skeletal muscle tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CDKN1B (10B7) Monoclonal Antibody (bsm-51281M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51281M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

KLF4 recombinant protein (20ug) probed with bsm-51264M KLF4 (5A1) Monoclonal Antibody at 1:4000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: KLF4 protein; probed with KLF4 (5A1) Monoclonal Antibody, unconjugated (bsm-51264M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-51264M

Applications: WB, IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-51203M

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-51197m-100ul

Applications: WB

Reactivity:

Conjugate: Unconjugated

SH-SY5Y (20\u00b5g) probed with bsm-51134M GABARAP (424CT5.1.6) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Mouse brain lysate (20\u00b5g) probed with bsm-51134M GABARAP (424CT5.1.6) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.

Catalog #1: bsm-51134M

Applications: WB, ELISA

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-2022M-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Virus

Conjugate: Biotin

Catalog #1: bsm-2022M-FITC

Applications: WB, IF(IHC-P)

Reactivity: Virus

Conjugate: FITC

Lane 1 : MCF7 Cell Lysate; Lane 2 : A431 Cell Lysate; Lane 3 : C6 Cell Lysate; Lane 4 : L929 Cell Lysate; probed with using bsm-50375M at Cyclin D1 (2G5) Monoclonal Antibody 4.0ug\/mL (1:250) and incubated at 4\u2103 overnight, followed by secondary antibody incubation for 60min at room temperature.
Lane 1 : Cyclin B1 Recombinant Protein; Lane 2 : Cyclin D1 Recombinant Protein; Lane 3 : Cyclin D2 Recombinant Protein; Lane 4 : Cyclin D3 Recombinant Protein; Lane 5 : Cyclin E1 Recombinant Protein; Lane 6 : Cyclin E2 Recombinant Protein; probed with using bsm-50375M at Cyclin D1 (2G5) Monoclonal Antibody 0.5ug\/mL (1:2000) and incubated at 4\u2103 overnight, followed by secondary antibody incubation for 60min at room temperature.

Catalog #1: bsm-50375M

Applications: WB, IP

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-2279M

Applications: ELISA, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Rat, Rabbit

Conjugate: Unconjugated

Catalog #1: bsm-1622M-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-2156M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-1622M-Cy3

Applications: WB, IF

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-9946R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-0297M

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-0107m-biotin-100ul

Applications: WB, IHC-P

Reactivity: Rat

Conjugate: Biotin

Catalog #1: bs-9972R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-9859R

Applications: ELISA, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Other Species

Conjugate: Unconjugated

Catalog #1: bsm-0954M-HRP

Applications: WB, IHC-P

Reactivity: Rat

Conjugate: HRP

Catalog #1: bs-9907R

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Antigen: bs-9826P, 2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1: 5000; \\nTMB staining\\nRead the data in Microplate Reader by 450nm. \\n
Mouse heart lysates probed with Anti C3orf21\/XXYLT1 Polyclonal Antibody, Unconjugated (bs-9826R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 43kD. Observed band size: 43kD.

Catalog #1: bs-9826R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Unconjugated

Catalog #1: bs-9878r

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-9688R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-2025M-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-9797R

Applications: WB, ELISA, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-9930R

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-9903R

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-9743R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-9929R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-9793R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-9926R

Applications: WB, ELISA, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse lung lysates probed with LAPTM4A Polyclonal Antibody, Unconjugated (bs-9562R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Uterus lysates; Lane 2: Mouse Ovary lysates probed with LAPTM4A Polyclonal Antibody, Unconjugated (bs-9562R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-9562R

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-9762R-HRP

Applications: WB, IHC-P

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-9814R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-9652R-FITC

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-9625R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TSSK6 Polyclonal Antibody, Unconjugated (bs-9734R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TSSK6 Polyclonal Antibody, Unconjugated (bs-9734R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-9734R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-9704R

Applications: ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-9622R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-9864R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity:

Conjugate: Unconjugated