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Catalog #1: bsm-33006M-Cy5

Applications: WB

Reactivity: Species independent

Conjugate: Cy5

Catalog #1: bsm-33006M-Cy5.5

Applications: WB

Reactivity: Species independent

Conjugate: Cy5.5

Catalog #1: bsm-33006M-Cy7

Applications: WB

Reactivity: Species independent

Conjugate: Cy7

Catalog #1: bsm-33006M-FITC

Applications: WB

Reactivity: Species independent

Conjugate: FITC

Catalog #1: bsm-33006M-HRP

Applications: WB, ELISA

Reactivity: Species independent

Conjugate: HRP

Catalog #1: bsm-33006M-PE

Applications: WB

Reactivity: Species independent

Conjugate: PE

Catalog #1: bsm-33006M-PE-Cy5

Applications: WB

Reactivity: Species independent

Conjugate: PE-Cy5

Catalog #1: bsm-33006M-PE-Cy5.5

Applications: WB

Reactivity: Species independent

Conjugate: PE-Cy5.5

Catalog #1: bsm-33006M-PE-Cy7

Applications: WB

Reactivity: Species independent

Conjugate: PE-Cy7

Catalog #1: bsm-33006M-PerCP

Applications: WB

Reactivity: Species independent

Conjugate: PerCP

Catalog #1: bsm-33006M-PerCP-Cy5.5

Applications: WB

Reactivity: Species independent

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-33006m-percp-cy7-100ul

Applications: WB

Reactivity: Species independent

Conjugate: PerCP-Cy7

Catalog #1: bsm-33006M-RBITC

Applications: WB

Reactivity: Species independent

Conjugate: RBITC

Catalog #1: bsm-51171M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: VWF protein; probed with VWF (907CT12.1.9) Monoclonal Antibody, unconjugated (bsm-51280M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with VWF (907CT12.1.9) Monoclonal Antibody (bsm-51280M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51280M

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-36205m

Applications: IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51649m

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-72466m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72467m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72468m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72469m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72470m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:10000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: 1: HeLa, 2: SH-SY5Y, 3: C2C12\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 3 s\r\nPredicted MW: 43 kDa\r\nObserved MW: 43 kDa\r\n
Tissue: Human liver cancer Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Mouse)(sp-0024) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60528M
Cell line: HeLa\r\nFixative: 4% Paraformaldehyde\r\nPermeabilization: 0.1% TritonX-100\r\nPrimary ab dilution: 1:50\r\nPrimary incubation condition: 4\u00b0C overnight\r\nSecondary ab: Goat Anti-Mouse IgG\r\nNuclear counter stain: DAPI (Blue)\r\nComment: Color green is the positive signal for bsm-60528M
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (WNT10B) Monoclonal Antibody, Unconjugated (bsm-60528M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-60528m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60493m

Applications: WB, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-51380M

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-51607m-100ul

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60571M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60343m

Applications: WB, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51723m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-72473m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72471m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72472m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72475m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72474m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-51340M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with YY1 (3C6) Monoclonal Antibody (bsm-51412M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: Daudi cell lysates; Lane 2: U-251 cell lysates; probed with YY1 (3C6) Polyclonal Antibody, unconjugated (bsm-51412M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Monkey brain tissue; Blocked with 3% BSA for 30min at RT; Antigen retrieval via heat mediation with a citrate buffer (pH6); Antibody incubation with YY1 (3C6) Monoclonal Antibody, Unconjugated (bsm-51412M) at 1:25 for 1hr at 37\u00b0C, followed by a conjugated secondary antibody and DAB staining.

Catalog #1: bsm-51412M

Applications: WB, FCM, IHC-P

Reactivity: Human, Rat, Monkey

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Monkey brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with YY1(Nuclear Loading Control) Monoclonal Antibody, Unconjugated (bsm-51566M) at 1:25 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with YY1(Nuclear Loading Control) Monoclonal Antibody, Unconjugated (bsm-51566M) at 1:25 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Zebrafish brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with YY1(Nuclear Loading Control) Monoclonal Antibody, Unconjugated (bsm-51566M) at 1:25 overnight at 4\u00b0C, DAB staining.
Lane 1: Daudi cell lysates; Lane 2: U251 cell lysates probed with YY1 Monoclonal Antibody, Unconjugated (bsm-51566M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51566m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Others

Conjugate: Unconjugated

ICC analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeled with Zap70 (4C2) Monoclonal Antibody (bsm-51298M) at 1:25 dilution, followed by secondary antibody incubation. Immunofluorescence image shows cytoplasm staining of Zap70 (green), actin (red), and nuclear counter stain with DAPI (blue).
Lane 1: Jurkat Cell lysates; Lane 2: MOLT-4 Cell lysates; Lane 3: mouse thymus Cell lysates; probed with Zap70 (4C2) Monoclonal Antibody, unconjugated (bsm-51298M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Jurkat cells probed with using Zap70 (4C2) Monoclonal Antibody, bsm-51298M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:400 (green) compared to an isotype control of mouse IgG2a (blue).

Catalog #1: bsm-51298M

Applications: WB, FCM, IHC-P, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51533m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51603m

Applications: WB, Flow-Cyt, ICC/IF

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-25842M

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-25842m-biotin

Applications: ELISA

Reactivity:

Conjugate: Biotin

Catalog #1: bsm-25842m-hrp

Applications: ELISA

Reactivity:

Conjugate: HRP

Catalog #1: bsm-51479m

Applications: WB, Flow-Cyt

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72476m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72477m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72478m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72479m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated