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Paraformaldehyde-fixed, paraffin embedded (human placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (PD-L1) Monoclonal Antibody, Unconjugated (bsm-54472R) at 1200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (PD-L1) Monoclonal Antibody, Unconjugated (bsm-54472R) at 1200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (PD-L1) Monoclonal Antibody, Unconjugated (bsm-54472R) at 1200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with PD-L1 monoclonal antibody, unconjugated (bsm-54472R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-54472r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney labeled with Cytochrome C (6B10) Monoclonal Antibody, Unconjugated (bsm-33193M) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytochrome C (6B10) Monoclonal Antibody, Unconjugated (bsm-33193M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytochrome C (6B10) Monoclonal Antibody, Unconjugated (bsm-33193M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytochrome C (6B10) Monoclonal Antibody, Unconjugated (bsm-33193M) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-33193m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Rat, Rabbit

Conjugate: Unconjugated

Lane 1: Mouse Placenta tissue lysates; Lane 2: Mouse Thymus tissue lysates; Lane 3: Mouse Lung tissue lysates; Lane 4: Rat Placenta tissue lysates probed with PD-1 Monoclonal Antibody, Unconjugated (bsm-43072M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PD-1 Monoclonal Antibody, Unconjugated (bsm-43072M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung squamous cell carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PD-1 Monoclonal Antibody, Unconjugated (bsm-43072M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Pyogenic appendicitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PD-1 Monoclonal Antibody, Unconjugated (bsm-43072M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-43072m

Applications: ELISA, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PD-1 Monoclonal Antibody, Unconjugated(bsm-41117M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen\r\n; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PD-1 Monoclonal Antibody, Unconjugated(bsm-41117M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.

Catalog #1: bsm-41117m

Applications: IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51506m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PSMA5 Monoclonal Antibody, Unconjugated (bsm-51520M) at 1:25 overnight at 4\u00b0C, DAB staining.
Lane 1: K562 cell lysates probed with PSMA5 Monoclonal Antibody, Unconjugated (bsm-51520M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human liver tissue lysates; Lane 2: Hela cell lysates; Lane 3: HepG2 cell lysates; Lane 4: MCF-7 cell lysates;Lane 5: Neuro-2a cell lysates; Lane 6: PC-12 cell lysates probed with PSMA5 Monoclonal Antibody, Unconjugated (bsm-51520M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51520m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60492m

Applications: WB, ICC/IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-60283M

Applications: FCM

Reactivity:

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with CD20 monoclonal antibody, unconjugated (bsm-33054M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD20 Monoclonal Antibody, Unconjugated (bsm-33054M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Diffuse large B-cell lymphoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD20 Monoclonal Antibody, Unconjugated (bsm-33054M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD20 Monoclonal Antibody, Unconjugated (bsm-33054M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-33054m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-43072m-cy3-100ul

Applications: IF

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-43072m-cy5-100ul

Applications: IF

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-43072m-Cy5.5-100ul

Applications: IF

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-43072m-cy7-100ul

Applications: IF

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-43072m-fitc-100ul

Applications: IF

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-43072m-pe-100ul

Applications: IF

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-43072m-biotin-100ul

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-43072m-hrp-100ul

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-43072m-bf350-100ul

Applications: IF

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-43072m-bf405-100ul

Applications: IF

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-43072m-bf488-100ul

Applications: IF

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-43072m-bf555-100ul

Applications: IF

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-43072m-bf594-100ul

Applications: IF

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bsm-43072m-bf647-100ul

Applications: IF

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bsm-43072m-bf680-100ul

Applications: IF

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bsm-43072m-bf750-100ul

Applications: IF

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bsm-43072m-apc-100ul

Applications: IF

Reactivity: Human

Conjugate: APC

Catalog #1: bsm-43072m-apc-Cy5.5-100ul

Applications: IF

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bsm-43072M-APC-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bsm-43072M-PE-Cy5.5

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bsm-43072M-PE-Cy5

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5

Catalog #1: bsm-43072M-PE-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bsm-43072m-percp-100ul

Applications: IF

Reactivity: Human

Conjugate: PerCP

Catalog #1: bsm-43072m-percp-Cy5.5-100ul

Applications: IF

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-43072m-rbitc-100ul

Applications: IF

Reactivity: Human

Conjugate: RBITC

Catalog #1: bsm-33193m-cy3-100ul

Applications: WB, IF

Reactivity: Human, Rat, Rabbit

Conjugate: Cy3

Catalog #1: bsm-33193m-cy5-100ul

Applications: WB, IF

Reactivity: Human, Rat, Rabbit

Conjugate: Cy5

Catalog #1: bsm-33193m-cy7-100ul

Applications: WB, IF

Reactivity: Human, Rat, Rabbit

Conjugate: Cy7

Catalog #1: bsm-33193m-fitc-100ul

Applications: WB, IF

Reactivity: Human, Rat, Rabbit

Conjugate: FITC

Catalog #1: bsm-33193m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Rat, Rabbit

Conjugate: Biotin

Catalog #1: bsm-33193m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Rat, Rabbit

Conjugate: HRP

Catalog #1: bsm-33193m-Cy5.5-100ul

Applications: WB, IF

Reactivity: Human, Rat, Rabbit

Conjugate: Cy5.5

Catalog #1: bsm-33193m-pe-100ul

Applications: WB, IF

Reactivity: Human, Rat, Rabbit

Conjugate: PE

Catalog #1: bsm-33193m-bf350-100ul

Applications: WB, IF

Reactivity: Human, Rat, Rabbit

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33193m-bf405-100ul

Applications: WB, IF

Reactivity: Human, Rat, Rabbit

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33193m-bf488-100ul

Applications: WB, IF

Reactivity: Human, Rat, Rabbit

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33193m-bf555-100ul

Applications: WB, IF

Reactivity: Human, Rat, Rabbit

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33193m-bf594-100ul

Applications: WB, IF

Reactivity: Human, Rat, Rabbit

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33193m-bf647-100ul

Applications: WB, IF

Reactivity: Human, Rat, Rabbit

Conjugate: AbBy Fluor-647