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CRISPR, Gene Editing & DNA-Repair Antibodies

CRISPR efficiency is only part of the result. Evaluate DNA-damage signaling, repair-pathway activation, and editing outcomes with antibodies targeting Cas9, RAD51, BRCA2, DNA-PK, γH2AX, ATM, ATR, and other key genome-stability proteins.



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CRC cell lysates probed with ATM (12A) Monoclonal Antibody (bsm-52360R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
IF(ICC) staining with ATM (12A) Monoclonal Antibody (bsm-52360R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with ATM (12A) Monoclonal Antibody (bsm-52360R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human liver cancer tissue incubated with ATM (12A) Monoclonal Antibody (bsm-52360R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52360r

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-9166R-HRP

Applications: WB, IHC-P

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-9166R-Biotin

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-9166R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-8510R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-8510R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-52163r-hrp

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-52163r-biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-9173R-HRP

Applications: WB, IHC-P

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-9173R-Biotin

Applications: WB, IHC-P

Reactivity: Mouse

Conjugate: Biotin

Catalog #1: bs-3185R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-3185R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Phospho-Chk1 (S296) Monoclonal Antibody, Unconjugated (bsm-54575R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Phospho-Chk1 (S296) Monoclonal Antibody, Unconjugated (bsm-54575R) at 1:50 for 30 minutes at room temperature, DAB staining.

Catalog #1: bsm-54575R

Applications: WB, IHC-P, IHC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-3721R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-3721R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-3015R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-13141R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-13141R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-5251R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-5251R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-5251R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-11226R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-11226R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-62071r-hrp

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-62071r-biotin

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

25 ug total protein per lane of various lysates (see on figure) probed with NHEJ1 monoclonal antibody, unconjugated (bsm-62071R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-NHEJ1 antibody (bsm-62071R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-62071r

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-13906R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-13906R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: Biotin

Catalog #1: bs-13906R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-5250R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-2400R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: HRP

Catalog #1: bs-2400R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-61302r-hrp

Applications: WB

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-61302r-biotin

Applications: WB, IP

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-61302r-TR

Applications: WB, IF(ICC), IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-8571R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: HRP

Catalog #1: bs-8571R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bs-13143R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Catalog #1: bs-13143R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bsm-61400r-hrp

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-61400r-biotin

Applications: ELISA, IHC-P, IHC-F, IP

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-61562r-hrp

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Western blot analysis of Jurkat cell lysate. Using Ctip2 (bsm-61400R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ctip2 Monoclonal Antibody, Unconjugated (bsm-61400R) at 1:300 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ctip2 Monoclonal Antibody, Unconjugated (bsm-61400R) at 1:300 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ctip2 Monoclonal Antibody, Unconjugated (bsm-61400R) at 1:300 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-61400r-TR

Applications: ELISA, IHC-P, IHC-F, IP, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1681R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-1681R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Hela cell lysate probed with Chk2 (7A4) Monoclonal Antibody (bsm-52522R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Chk2 (7A4) Monoclonal Antibody (bsm-52522R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Chk2 (7A4) Monoclonal Antibody (bsm-52522R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Chk2 (7A4) Monoclonal Antibody (bsm-52522R) at 1:100 in 293 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52522R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-11544R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-61562r-biotin

Applications: WB, IHC-P, IHC-F, IP

Reactivity: Human

Conjugate: Biotin