CRISPR, Gene Editing & DNA-Repair Antibodies

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CRISPR efficiency is only part of the result. Evaluate DNA-damage signaling, repair-pathway activation, and editing outcomes with antibodies targeting Cas9, RAD51, BRCA2, DNA-PK, γH2AX, ATM, ATR, and other key genome-stability proteins.

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Lane 1: 293T cells transfected with CRISPR9-Cas9; Lane 2: untransfected 293T cell lysate probed with CRISPR-Cas9 (1E8) Monoclonal Antibody, Unconjugated (bsm-52042M) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody. — Cas9 (1E8) Monoclonal Antibody

Catalog #1: bsm-52042m

Applications: WB, ICC/IF

Reactivity: Human, Others

Conjugate: Unconjugated

Lane 1: Jurkat Cell lysates; Lane 2: NIH\/3T3 Cell lysates; Lane 3: C6 Cell lysates; Lane 4: PC-12 Cell lysates; Lane 5: Hela Cell lysates; probed with RAD51 (1C9) Monoclonal Antibody, unconjugated (bsm-51402M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C. — RAD51 (1C9) Monoclonal Antibody

Catalog #1: bsm-51402M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: Mouse NIH\/3T3 cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human Jurkat cell lysates; Lane 4: Human Raji cell lysates probed with RAD5 Monoclonal Antibody, Unconjugated (bsm-51402M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C. — RAD51 (1C9) Monoclonal Antibody

Catalog #1: bsm-51402M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: 293 Cell lysates; Lane 2: CEM Cell lysates; Lane 3: HepG2 Cell lysates; Lane 4: Jurkat Cell lysates; Lane 5: HeLa Cell lysates; Lane 6: Raji Cell lysates; probed with H2AFX (8C5) Monoclonal Antibody, unconjugated (bsm-51222M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C. — H2AX (8C5) Monoclonal Antibody

Catalog #1: bsm-51222M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Thymus tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with H2AFX (8C5) Monoclonal Antibody (bsm-51222M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining. — H2AX (8C5) Monoclonal Antibody

Catalog #1: bsm-51222M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

\r\nBlocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: room temperature 2h\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: 293, A431, MDA-MB-231, SH-SY5Y\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 55 kDa\r\nObserved MW: 55 kDa\r\n — XRCC4 Monoclonal Antibody

Catalog #1: bsm-60571M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: 4\u00b0C overnight\r\nLysate: HeLa, Jurkat, A549\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 86 kDa\r\nObserved MW: 86 kDa — XRCC5 Monoclonal Antibody

Catalog #1: bsm-60343m

Applications: WB, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: A431; Lane 2: HeLa; Lane 3: Jurkat; Lane 4: K562; Lane 5: MCF-7 cell lysates at 20ug per lane; probed with bsm-51266M CHK1 (2G1D5) Monoclonal Antibody at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C. — CHK1 (2G1D5) Monoclonal Antibody

Catalog #1: bsm-51266M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with CHEK2 monoclonal antibody, unconjugated (bsm-60559M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min. — CHEK2 Monoclonal Antibody

Catalog #1: bsm-60559m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

CRISPR Cas9 Antibody

Catalog #1: bsm-70054M

Applications: WB, IHC

Reactivity: Others

Conjugate: Unconjugated

Lane 1: Recombinant protein lysates probed with BRCA2 Monoclonal Antibody, Unconjugated (bsm-51693M) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C. — BRCA2 Monoclonal Antibody

Catalog #1: bsm-51693m

Applications: WB

Reactivity: Recombinant protein

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nLysate: HeLa, MCF7, HepG2, A549, Jurkat\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 180 s\r\nPredicted MW: 300 kDa\r\nObserved MW: 240 kDa — ATR Monoclonal Antibody

Catalog #1: bsm-60260M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

ATM (C-terminal region) Antibody

Catalog #1: bsm-70350M

Applications: WB, FCM, IP

Reactivity: Human

Conjugate: Unconjugated

ATM (Ser-1981), Phosphospecific Antibody

Catalog #1: bsm-70352M

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated