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Endothelial Cell Type Markers



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Catalog #1: bs-1618R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bs-1618R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human, Rat

Conjugate: HRP

Catalog #1: bs-5450R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-5450R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

IF(ICC) staining with ICAM1 (1G2) Monoclonal Antibody (bsm-52466R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with ICAM1 (1G2) Monoclonal Antibody (bsm-52466R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ICAM1 Monoclonal Antibody, Unconjugated(bsm-52466R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ICAM1 Monoclonal Antibody, Unconjugated(bsm-52466R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.

Catalog #1: bsm-52466r-TR

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-12262R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-13073R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-13074R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy5.5

Catalog #1: bs-1300R-Cy5.5

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy5.5

Catalog #1: bs-0468R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Dog

Conjugate: Cy5.5

Catalog #1: bs-4754R-Cy5.5

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-4609R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-0646r-Cy5.5-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-3589R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-3731R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy5.5

Catalog #1: bs-0163R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy5.5

Catalog #1: bs-10412R-Cy5.5

Applications: FCM, IF(IHC-P)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-2089R-Cy5.5

Applications: WB, FCM

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-2674R-Cy5.5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-3449R-Cy5.5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy5.5

Catalog #1: bs-4808R-Cy5.5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-5838R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Cy5.5

Catalog #1: bs-8996R-Cy5.5

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Cy5.5

Catalog #1: bsm-0954M-Cy5.5

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: Cy5.5

Catalog #1: bs-0195R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-0195R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-0195R-Cy3

Applications: WB, FCM, IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-0195R-Cy5

Applications: WB, FCM, IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-0195R-Cy5.5

Applications: WB, FCM, IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-0195R-PE

Applications: WB, FCM, IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: PE

Catalog #1: bs-0954R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-0954R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-0954R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-0954R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-0954R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-0954R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-0954R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ICAM1\/CD54 Monoclonal Antibody, Unconjugated (bsm-10697M) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Human A549 cell lysates; Lane 2: Human MCF-7 cell lysates probed with ICAM1\/CD54 Monoclonal Antibody, Unconjugated (bsm-10697M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human lung squamous cell carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ICAM1\/CD54 Monoclonal Antibody, Unconjugated (ascites of bsm-10697M) at 1:800 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-10697m

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-10697m-biotin-100ul

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-10697m-hrp-100ul

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-33176M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33176M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33176M-Cy5

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33176M-Cy3

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33176M-Cy5.5

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-33176M-Cy7

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-33176M-FITC

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: FITC

HUVEC cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD144\/VE Cadherin Polyclonal Antibody(bs-22363R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
HUVEC cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD144\/VE Cadherin Polyclonal Antibody(bs-22363R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Human MCF-7 cell lysates; Lane 2: Human HUVEC cell lysates; Lane 3: Human A431 cell lysates; Lane 4: Human HeLa cell lysates probed with CD144\/VE Cadherin Polyclonal Antibody, Unconjugated (bs-22363R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD144\/VE Cadherin) Polyclonal Antibody, Unconjugated (bs-22363R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-22363R

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: Unconjugated