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Primary Antibodies

Primary Antibodies

Our Primary Antibodies are research-use antibodies designed to support protein detection and target analysis across a wide range of applications and species. Our collection includes recombinant antibodies, monoclonal antibodies, and polyclonal antibodies for use in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), flow cytometry (FC), ELISA, and other common applications.

These primary antibodies are available for targets involved in cancer research, immunology, neuroscience, cell biology, metabolism, apoptosis, signaling pathways, and disease-related research. Bioss offers primary antibodies guaranteed for use in human, mouse, rat, and other species to help researchers choose the right antibody format for their experimental model and detection method.



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Catalog #1: bs-51092r-50ug

Applications: WB, ELISA, IF, dot-blot

Reactivity: Human, Mouse, Others

Conjugate: Unconjugated

Catalog #1: bs-51141r-50ug

Applications: WB, ELISA, IF, dot-blot

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-51080R

Applications: ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51070r-100ul

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51146r-50ug

Applications: WB, ELISA, dot-blot

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51087R

Applications: ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51068r-100ul

Applications: WB, ELISA

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-51088R

Applications: ELISA

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-51058r-100ul

Applications: WB, ELISA

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-51079R

Applications: ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51067r-100ul

Applications: WB, ELISA

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bsm-53023M

Applications: WB, ELISA, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-53141R

Applications: WB, ELISA, IF, dot-blot

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-53142R

Applications: WB, ELISA, IF, dot-blot

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-53070R

Applications: ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-53087R

Applications: ELISA

Reactivity: Human

Conjugate: Unconjugated

NIH3T3 cells were stained with the Ash2 antibody (bs-53055R) and with DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the Ash2 antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two is shown on the right.
To determine the titer, an ELISA was performed using a serial dilution of the Ash2 antibody (bs-53055R). The wells were coated with the peptides used for immunization of the host rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:24,000.

Catalog #1: bs-53055R

Applications: WB, ELISA, IF

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-24918R-Biotin

Applications: WB, ELISA

Reactivity: Mouse

Conjugate: Biotin

Catalog #1: bs-24918R-HRP

Applications: WB, ELISA

Reactivity: Mouse

Conjugate: HRP

Sample:\r\nLane 1: Mouse Uterus tissue lysates\r\nLane 2: Mouse Small intestine tissue lysates\r\nLane 3: Mouse Kidney tissue lysates\r\nLane 4: Mouse Prostate tissue lysates\r\nPrimary: Anti-EP4 (bs-24918R) at 1\/1000 dilution\r\nSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1\/20000 dilution\r\nPredicted band size: 53 kD\r\nObserved band size: 55 kD
Ctll-2 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Prostaglandin E Receptor EP4 Antibody(bs-24918R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
SP2\/0 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (EP4) polyclonal Antibody, Unconjugated (bs-24918R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Mouse Uterus tissue lysates; Lane 2: Mouse Small intestine tissue lysates; Lane 3: Mouse Kidney tissue lysates; Lane 4: Mouse Prostate tissue lysates probed with EP4 Polyclonal Antibody, Unconjugated (bs-24918R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-24918R

Applications: WB, ELISA, FCM

Reactivity: Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (mouse heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (muscle Actin) Polyclonal Antibody, Unconjugated (bs-13947R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (muscle Actin) Polyclonal Antibody, Unconjugated (bs-13947R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (muscle Actin) Polyclonal Antibody, Unconjugated (bs-13947R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with muscle Actin polyclonal antibody, unconjugated (bs-13947R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bs-13947r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-41622M

Applications: ELISA

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bs-24804R

Applications: WB, ELISA

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-24804R-Biotin

Applications: WB, ELISA

Reactivity: Mouse

Conjugate: Biotin

Catalog #1: bs-24804R-HRP

Applications: WB, ELISA

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-23966R

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-23966R-Biotin

Applications: WB, ELISA

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-23966R-HRP

Applications: WB, ELISA

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-24944R

Applications: WB, ELISA

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-24944R-Biotin

Applications: WB, ELISA

Reactivity: Rat

Conjugate: Biotin

Catalog #1: bs-24944R-HRP

Applications: WB, ELISA

Reactivity: Rat

Conjugate: HRP

Catalog #1: bs-43004R

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-43004R-Biotin

Applications: WB, ELISA

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-43004R-HRP

Applications: WB, ELISA

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-43011R

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-43011R-Biotin

Applications: WB, ELISA

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-43011R-HRP

Applications: WB, ELISA

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-43009R-Biotin

Applications: WB, ELISA

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-43009R

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-43009R-HRP

Applications: WB, ELISA

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-24783R

Applications: WB, ELISA

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-24783R-Biotin

Applications: WB, ELISA

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-24783R-HRP

Applications: WB, ELISA

Reactivity: Mouse, Rat

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (MYH7) Polyclonal Antibody, Unconjugated (bs-24941R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (MOUSE skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (MYH7) Polyclonal Antibody, Unconjugated (bs-24941R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (MYH7) Polyclonal Antibody, Unconjugated (bs-20941R) at 1:200 overnight at 4\u00b0C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.

Catalog #1: bs-24941R

Applications: ELISA, IHC-P, IF

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-24941R-Biotin

Applications: ELISA, IHC-P

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-24941R-HRP

Applications: ELISA, IHC-P

Reactivity: Mouse, Rat

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (TNFR1) Polyclonal Antibody, Unconjugated (bs-22306R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (TNFR1) Polyclonal Antibody, Unconjugated (bs-22306R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (TNFR1) Polyclonal Antibody, Unconjugated (bs-22306R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-22306r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-22306R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP