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Primary Antibodies

Primary Antibodies

Our Primary Antibodies are research-use antibodies designed to support protein detection and target analysis across a wide range of applications and species. Our collection includes recombinant antibodies, monoclonal antibodies, and polyclonal antibodies for use in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), flow cytometry (FC), ELISA, and other common applications.

These primary antibodies are available for targets involved in cancer research, immunology, neuroscience, cell biology, metabolism, apoptosis, signaling pathways, and disease-related research. Bioss offers primary antibodies guaranteed for use in human, mouse, rat, and other species to help researchers choose the right antibody format for their experimental model and detection method.



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Catalog #1: bs-21401R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-21401R-HRP

Applications: WB

Reactivity: Human

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD34 Monoclonal Antibody, Unconjugated (bsm-54470R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD34) Monoclonal Antibody, Unconjugated (bsm-54470R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD34) Monoclonal Antibody, Unconjugated (bsm-54470R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD34) Monoclonal Antibody, Unconjugated (bsm-54470R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bsm-54470r-100ul

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with phospho-IRS1 (Tyr632) Polyclonal Antibody, Unconjugated (bs-8707R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with phospho-IRS1 (Tyr632) Polyclonal Antibody, Unconjugated (bs-8707R) at 1:200 overnight at 4\u00b0C, DAB staining.
A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (phospho-IRS1 (Tyr632) ) polyclonal Antibody, Unconjugated (bs-8707R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-8707R

Applications: IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit

Conjugate: Unconjugated

Catalog #1: bs-8707R-Biotin

Applications: IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit

Conjugate: Biotin

Catalog #1: bs-8707R-HRP

Applications: IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit

Conjugate: HRP

Catalog #1: bs-8707R-Cy3

Applications: IF

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit

Conjugate: Cy3

Catalog #1: bs-8707R-Cy5

Applications: IF

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit

Conjugate: Cy5

Catalog #1: bs-8707R-Cy5.5

Applications: IF

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit

Conjugate: Cy5.5

Catalog #1: bs-8707R-Cy7

Applications: IF

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit

Conjugate: Cy7

Catalog #1: bs-8707R-FITC

Applications: IF

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit

Conjugate: FITC

Catalog #1: bs-8707R-PE

Applications: IF

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit

Conjugate: PE

Catalog #1: bsm-33412M

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig

Conjugate: Unconjugated

Catalog #1: bsm-33412M-Biotin

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig

Conjugate: Biotin

Catalog #1: bsm-33412M-HRP

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig

Conjugate: HRP

Ctll-2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Bcl-2 monoclonal Antibody(bsm-33413M)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Ctll-2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Bcl-2 monoclonal Antibody(bsm-33413M)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bsm-33413M

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig

Conjugate: Unconjugated

Catalog #1: bsm-33413M-HRP

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig

Conjugate: HRP

Catalog #1: bsm-33413M-Biotin

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig

Conjugate: Biotin

Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RSPO1 Polyclonal Antibody, Unconjugated (bs-21385R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RSPO1 Polyclonal Antibody, Unconjugated (bs-21385R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-21385R

Applications: IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Rabbit, Horse

Conjugate: Unconjugated

Catalog #1: bs-21385R-Biotin

Applications: IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Rabbit, Horse

Conjugate: Biotin

Catalog #1: bs-21385R-HRP

Applications: IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Rabbit, Horse

Conjugate: HRP

Catalog #1: bs-1640R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bs-4727R-PE

Applications: WB, FCM

Reactivity: Human

Conjugate: PE

Catalog #1: bs-0042R-PE

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: PE

Catalog #1: bs-3330R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bs-3016R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bs-5551R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bs-0061R-PE

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: PE

Catalog #1: bs-0439R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: PE

Catalog #1: bs-10233r-pe-100ul

Applications: WB, FCM, IF, Flow-Cyt

Reactivity: Human

Conjugate: PE

Catalog #1: bs-1531R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bs-3242R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: PE

Catalog #1: bs-3737R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: PE

Catalog #1: bs-4512R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bs-0023R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-0023R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-0023R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-0023R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-0023R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-0501R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bs-2235R-PE

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PE

Catalog #1: bs-18423R-Cy5.5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy5.5

Catalog #1: bs-18423R-Cy7

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy7

Catalog #1: bs-18423R-Cy3

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy3

Catalog #1: bs-18423R-FITC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: FITC

Catalog #1: bs-18423R-Cy5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy5

Catalog #1: bs-9439R-PE

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Rat

Conjugate: PE

Catalog #1: bs-4609R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: PE