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Primary Antibodies

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Lane 1: rat brain lysates Lane 2: rat kidney lysates probed with Anti MCR Polyclonal Antibody, Unconjugated (bs-1850R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 108kD. Observed band size: 108kD.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Mineralocorticoid receptor Polyclonal Antibody, Unconjugated (bs-1850R) at 1:400 overnight at 4\u00b0C, followed by secondary and DAB staining.
Molt-4 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with \r\nMineralocorticoid receptor Polyclonal Antibody(bs-1850R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Mouse spleen cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Mineralocorticoid receptor Polyclonal Antibody(bs-1850R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-1850R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated

Catalog #1: bs-1850R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Biotin

Catalog #1: bs-1850R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog

Conjugate: HRP

Catalog #1: bs-1850R-A488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 488

Catalog #1: bs-1850R-A350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 350

Catalog #1: bs-1850R-A680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 680

Catalog #1: bs-1850R-A555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 555

Catalog #1: bs-1850R-A594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 594

Catalog #1: bs-1850R-A647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 647

Catalog #1: bs-1850R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy3

Catalog #1: bs-1850R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy5

Catalog #1: bs-1850R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy7

Catalog #1: bs-1850R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: FITC

Catalog #1: bs-1850R-A750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 750

Catalog #1: bs-1948R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Biotin

Frozen mouse brain sections labeled with Anti-WNT5A Polyclonal Antibody, Unconjugated (bs-1948R) at 1:1000 followed by conjugation to the secondary antibody
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-WNT5A Polyclonal Antibody, Unconjugated (bs-1948R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Image kindly provided by Dr. Magdalena Krol. Control tumor cells, tumor cells grown in macrophage-conditioned medium, tumor cells sorted from co-culture with macrophages, and macrophages from monocultures and sorted from co-culture with tumor cells were analyzed. Total protein concentrations in lysates were determined using a Bio-Rad protein assay. Proteins (50 mg) were resolved using SDS-PAGE and transferred onto PVDF membranes. The membranes were then blocked with 5% non-fat dry milk in TBS buffer containing 0.5% Tween 20. The membranes were then incubated overnight with the primary Rabbit Anti-WNT5A Polyclonal Antibody at 1:100 dilution. Subsequently, the membranes were washed three times in TBS containing 0.5% Tween 20 and incubated for 1 h at room temperature with secondary antibodies conjugated with the appropriate infrared (IR) fluorophore IRDyeH 800 CW or IRDyeH 680 RD at a dilution of 1:5000.
Image kindly submitted by Dr. Magdalena Krol. Formalin-fixed and paraffin embedded canine mammary tumor labeled with Rabbit Anti-WNT5A Polyclonal Antibody, Unconjugated (bs-1948R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-1948R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated

Catalog #1: bs-1948R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog

Conjugate: HRP

Catalog #1: bs-1948R-A350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 350

Catalog #1: bs-1948R-A488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 488

Catalog #1: bs-1948R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy3

Catalog #1: bs-1948R-A555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 555

Catalog #1: bs-1948R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy5

Catalog #1: bs-1948R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: FITC

Catalog #1: bs-1948R-A647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 647

Catalog #1: bs-1948R-A594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 594

Catalog #1: bs-1948R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy7

Catalog #1: bs-1948R-A750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 750

Catalog #1: bs-1948R-A680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 680

Image kindly provided by Dr. Magdalena Krol. Control tumor cells, tumor cells grown in macrophage-conditioned medium, tumor cells sorted from co-culture with macrophages, and macrophages from monocultures and sorted from co-culture with tumor cells were analyzed. Total protein concentrations in lysates were determined using a Bio-Rad protein assay. Proteins (50 mg) were resolved using SDS-PAGE and transferred onto PVDF membranes. The membranes were then blocked with 5% non-fat dry milk in TBS buffer containing 0.5% Tween 20. The membranes were then incubated overnight with the primary Rabbit Anti-DKK1 Polyclonal Antibody at 1:100 dilution. Subsequently, the membranes were washed three times in TBS containing 0.5% Tween 20 and incubated for 1 h at room temperature with secondary antibodies conjugated with the appropriate infrared (IR) fluorophore IRDyeH 800 CW or IRDyeH 680 RD at a dilution of 1:5000.
Image kindly submitted by Dr. Magdalena Krol. Formalin-fixed and paraffin embedded canine mammary tumor labeled with Rabbit Anti-DKK1 Polyclonal Antibody, Unconjugated (bs-2162R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-2162R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Dog

Conjugate: Unconjugated

Catalog #1: bs-2162R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Dog

Conjugate: HRP

Catalog #1: bs-2162R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Dog

Conjugate: Biotin

Catalog #1: bs-2162R-A350

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Dog

Conjugate: ALEXA FLUOR® 350

Catalog #1: bs-2162R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Dog

Conjugate: FITC

Catalog #1: bs-2162R-A488

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Dog

Conjugate: ALEXA FLUOR® 488

Catalog #1: bs-2162R-A555

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Dog

Conjugate: ALEXA FLUOR® 555

Catalog #1: bs-2162R-A647

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Dog

Conjugate: ALEXA FLUOR® 647

Catalog #1: bs-2162R-A594

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Dog

Conjugate: ALEXA FLUOR® 594

Catalog #1: bs-2162R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Dog

Conjugate: Cy3

Catalog #1: bs-2162R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Dog

Conjugate: Cy5

Catalog #1: bs-2162R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Dog

Conjugate: Cy7

Catalog #1: bs-2162R-A750

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Dog

Conjugate: ALEXA FLUOR® 750

Catalog #1: bs-2162R-A680

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Dog

Conjugate: ALEXA FLUOR® 680

Catalog #1: bs-2242R-A350

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Dog, Rabbit

Conjugate: ALEXA FLUOR® 350

Formalin-fixed and paraffin embedded rabbit alveolar bone labeled with Anti-BMP7 Polyclonal Antibody, Unconjugated (bs-2242R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rabbit alveolar bone tissue labeled with Anti-BMP7 Polyclonal Antibody, Unconjugated (bs-2242R) at 1:300 followed by conjugation to the secondary antibody
Mouse bone lysates probed with BMP7 Polyclonal Antibody, Unconjugated (bs-2242R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Kidney lysates; Lane 2: Mouse Placenta lysates; Lane 3: Mouse Cerebrum lysates; Lane 4: Mouse NIH\/3T3 cell lysates; Lane 5: Rat Cerebrum lysates probed with BMP7 Polyclonal Antibody, Unconjugated (bs-2242R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2242R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Dog, Rabbit

Conjugate: Unconjugated

Catalog #1: bs-2242R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat, Dog, Rabbit

Conjugate: Biotin

Catalog #1: bs-2242R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat, Dog, Rabbit

Conjugate: HRP

Catalog #1: bs-2242R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Dog, Rabbit

Conjugate: Cy7

Catalog #1: bs-2242R-A647

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Dog, Rabbit

Conjugate: ALEXA FLUOR® 647