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Primary Antibodies

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Catalog #1: bs-2242R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Dog, Rabbit

Conjugate: Cy5

Catalog #1: bs-2242R-A594

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Dog, Rabbit

Conjugate: ALEXA FLUOR® 594

Catalog #1: bs-2242R-A555

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Dog, Rabbit

Conjugate: ALEXA FLUOR® 555

Catalog #1: bs-2242R-A488

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Dog, Rabbit

Conjugate: ALEXA FLUOR® 488

Catalog #1: bs-2242R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Dog, Rabbit

Conjugate: Cy3

Catalog #1: bs-2242R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Dog, Rabbit

Conjugate: FITC

Catalog #1: bs-2242R-A680

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Dog, Rabbit

Conjugate: ALEXA FLUOR® 680

Catalog #1: bs-2242R-A750

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Dog, Rabbit

Conjugate: ALEXA FLUOR® 750

Formalin-fixed and paraffin embedded human glioma tissue labeled with Anti-SIRT1 Polyclonal Antibody, Unconjugated (bs-2257R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SIRT1\/sirtuin 1 Polyclonal Antibody, Unconjugated (bs-2257R) at 1:400 overnight at 4\u00b0C, DAB staining.
HL-60 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with SIRT1 Polyclonal Antibody(bs-2257R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Hela cell lysates; Lane 2: NIH\/3T3 cell lysates; Lane 3: HepG2 cell lysates; Lane 4: 293T cell lysates ; Lane 5: Mouse Testis lysates probed with SIRT1\/sirtuin 1 Polyclonal Antibody, Unconjugated (bs-2257R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2257R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Dog

Conjugate: Unconjugated

Catalog #1: bs-2257R-A350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Dog

Conjugate: ALEXA FLUOR® 350

Catalog #1: bs-2257R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Dog

Conjugate: HRP

Catalog #1: bs-2257R-A488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Dog

Conjugate: ALEXA FLUOR® 488

Catalog #1: bs-2257R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Dog

Conjugate: Biotin

Catalog #1: bs-2257R-A647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Dog

Conjugate: ALEXA FLUOR® 647

Catalog #1: bs-2257R-A555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Dog

Conjugate: ALEXA FLUOR® 555

Catalog #1: bs-2257R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Dog

Conjugate: Cy5

Catalog #1: bs-2257R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Dog

Conjugate: Cy7

Catalog #1: bs-2257R-A594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Dog

Conjugate: ALEXA FLUOR® 594

Catalog #1: bs-2257R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Dog

Conjugate: Cy3

Catalog #1: bs-2257R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Dog

Conjugate: FITC

Catalog #1: bs-2257R-A680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Dog

Conjugate: ALEXA FLUOR® 680

Catalog #1: bs-2257R-A750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Dog

Conjugate: ALEXA FLUOR® 750

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti DGAT1\/Diglyceride acyltransferase Polyclonal Antibody, Unconjugated (bs-2332R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti DGAT1\/Diglyceride acyltransferase Polyclonal Antibody, Unconjugated (bs-2332R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with DGAT1 Polyclonal Antibody, Unconjugated (bs-2332R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Lane 1: Mouse Large intestine lysates; Lane 2: Mouse intestine lysates probed with DGAT1 Polyclonal Antibody, Unconjugated (bs-2332R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2332R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated

Catalog #1: bs-2332R-A555

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 555

Catalog #1: bs-2332R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog

Conjugate: HRP

Catalog #1: bs-2332R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Biotin

Catalog #1: bs-2332R-A350

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 350

Catalog #1: bs-2332R-A488

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 488

Catalog #1: bs-2332R-A594

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 594

Catalog #1: bs-2332R-A647

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 647

Catalog #1: bs-2332R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy3

Catalog #1: bs-2332R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy5

Catalog #1: bs-2332R-A680

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 680

Catalog #1: bs-2332R-A750

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 750

Catalog #1: bs-2332R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy7

Catalog #1: bs-2332R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: FITC

Staining of canine lymph node tissue with bs-3077r. The primary antibody bs-3077r was used at a 100 fold (A and B) and 500 fold (C and D) dilution. A no primary antibody negative control (E and F) was used to verify the specificity of the stain. The blocking step with avidin and biotin was omitted for the no primary antibody negative controls. As a result, there is some unspecific staining in these sections. \r\nImages in the upper row (A, C, and E) were captured at 10x magnification, while the images below (B, D, and F) were taken at 20x magnification. Image was kindly submitted by a researcher via the Antibodies Online Independent Validation Program. \r\n
Raw264.7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with phospho-MCSF Receptor (Tyr723) Polyclonal Antibody(bs-3077R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-3077R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Dog

Conjugate: Unconjugated

Catalog #1: bs-3077R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Dog

Conjugate: HRP

Catalog #1: bs-3077R-A350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Dog

Conjugate: ALEXA FLUOR® 350

Catalog #1: bs-3077R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Dog

Conjugate: Biotin

Catalog #1: bs-3077R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Dog

Conjugate: FITC

Catalog #1: bs-3077R-A594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Dog

Conjugate: ALEXA FLUOR® 594

Catalog #1: bs-3077R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Dog

Conjugate: Cy3

Catalog #1: bs-3077R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Dog

Conjugate: Cy5

Catalog #1: bs-3077R-A750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Dog

Conjugate: ALEXA FLUOR® 750

Catalog #1: bs-3077R-A680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Dog

Conjugate: ALEXA FLUOR® 680

Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-Phospho-c-Kit(Tyr721) Polyclonal Antibody, Unconjugated (bs-3242R) followed by conjugation to the secondary antibody and DAB staining
Molt-4 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with c-Kit(Tyr721) Polyclonal Antibody(bs-3242R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \r\nc-Kit(Tyr721) Polyclonal Antibody, Unconjugated (bs-3242R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3242R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated

Catalog #1: bs-3242R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Biotin