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Primary Antibodies

Primary Antibodies

Our Primary Antibodies are research-use antibodies designed to support protein detection and target analysis across a wide range of applications and species. Our collection includes recombinant antibodies, monoclonal antibodies, and polyclonal antibodies for use in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), flow cytometry (FC), ELISA, and other common applications.

These primary antibodies are available for targets involved in cancer research, immunology, neuroscience, cell biology, metabolism, apoptosis, signaling pathways, and disease-related research. Bioss offers primary antibodies guaranteed for use in human, mouse, rat, and other species to help researchers choose the right antibody format for their experimental model and detection method.



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HT-1080 Cells lysates probed with MMP14 (3A6) Monoclonal Antibody, unconjugated (bsm-51028M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at Room Temperature.
Lane 1: A2058 Cell lysates; probed with MMP14 (3A6) Monoclonal Antibody, unconjugated (bsm-51028M) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HepG2 cells; Antibody incubation with MMP14 (3A6) Monoclonal Antibody, Unconjugated (bsm-51028M) at 1:25 overnight at 4\u00b0C, followed by a conjugated secondary DAPI and for nuclei staining.

Catalog #1: bsm-51028M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51046M

Applications: WB, IHC-P, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51059M

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Lane 1: HepG2 Cell lysates; Lane 2: MCF-7 Cell lysates; probed with MAP4K3 (219CT8.3.1) Monoclonal Antibody, unconjugated (bsm-51067M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human skeletal muscle tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with MAP4K3 (219CT8.3.1) Monoclonal Antibody (bsm-51067M) at 1:100 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51067M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51068M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51079M

Applications: WB, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51083M

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51086M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51089M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with HLA-DRA monoclonal antibody, unconjugated (bsm-51091M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with HLA-DRA Monoclonal Antibody, Unconjugated(bsm-51091M) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with HLA-DRA Monoclonal Antibody, Unconjugated(bsm-51091M) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Esophagus Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with HLA-DRA Monoclonal Antibody, Unconjugated(bsm-51091M) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-51091m-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51093M

Applications: WB, IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51103M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51113M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51117M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51114M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51118M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51120M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51121M

Applications: WB

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bsm-51122M

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51128M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51159M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51158M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51168M

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-51160M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51161M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51162m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51165M

Applications: WB

Reactivity:

Conjugate: Unconjugated

HeLa cell lysates; probed with STRA8 (526CT11.1.5) Monoclonal Antibody, unconjugated (bsm-51172M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Mouse bladder lysates; probed with STRA8 (526CT11.1.5) Monoclonal Antibody, unconjugated (bsm-51172M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-51172M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51171M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51183M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51190M

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51191M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51197m-100ul

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-51200M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51206M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: 293 Cell lysates; Lane 2: K562 Cell lysates; Lane 3: MCF-7 Cell lysates; Lane 4: SK-BR-3 Cell lysates; Lane 5: T47D Cell lysates; Lane 6: MDA-MB-453 Cell lysates; Lane 7: mouse testis lysates; probed with ACE2 (881CT16.4.4) Monoclonal Antibody, unconjugated (bsm-51221M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded (Human kidney ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ACE2(1C4) ) Monoclonal Antibody, Unconjugated (bsm-51221M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ACE2(1C4) ) Monoclonal Antibody, Unconjugated (bsm-51221M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ACE2(1C4) ) Monoclonal Antibody, Unconjugated (bsm-51221M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-51221M

Applications: WB, ELISA, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51227M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51228M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51266M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-52042m-100ul

Applications: WB, ICC/IF

Reactivity: Human, Others

Conjugate: Unconjugated

Catalog #1: bsm-51492m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51507m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Mouse brain tissue lysates probed with Irgm1 Monoclonal Antibody, Unconjugated (bsm-51508M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse brain tissue lysates; Lane 2: Mouse Heart tissue lysates probed with Irgm1 Monoclonal Antibody, Unconjugated (bsm-51508M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51508m

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

The TT (treated with BFA ) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Mouse Anti-CALCA antibody (bsm-51512M,1:100); Isotype Control (orange): Mouse IgG (bs-0296P). Blank control (black): PBS. Acquisition of 20,000 events was performed.\r\n
25 ug total protein per lane of various lysates (see on figure) probed with CALCA monoclonal antibody, unconjugated (bsm-51512M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-51512m-100ul

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51522m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP3A4 Monoclonal Antibody, Unconjugated (bsm-51529M) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP3A4 Monoclonal Antibody, Unconjugated (bsm-51529M) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP3A4 Monoclonal Antibody, Unconjugated (bsm-51529M) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP3A4 Monoclonal Antibody, Unconjugated (bsm-51529M) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-51529m-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Lane 1: HepG2 cell lysates probed with THAP11 Monoclonal Antibody, Unconjugated (bsm-51505M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: NIH\/3T3 cell lysates probed with THAP11 Monoclonal Antibody, Unconjugated (bsm-51505M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51505m

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (human CD15 (SSEA-1) ) Monoclonal Antibody, Unconjugated (bsm-30016M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (human CD15 (SSEA-1) ) Monoclonal Antibody, Unconjugated (bsm-30016M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
scatter diagram showing peripheral blood lymphocytes stained with CD15\/SSEA1. The cells were incubated with the antibody (bsm-30016M) for 30 min at 22\u00b0C.The secondary antibody used for 40 min at room temperature. Acquisition of >10,000 events was performed.

Catalog #1: bsm-30016m-100ul

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated