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Catalog #1: bs-11179R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human cervical carcinoma labeled with Anti-IGFBP2 Polyclonal Antibody, Unconjugated (bs-1108R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human glioma labeled with Anti-IGFBP2 Polyclonal Antibody, Unconjugated (bs-1108R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: Rat Brain lysates; Lane 2: Human Colon Carcinoma lysates; Probed with IGFBP2 Polyclonal Antibody, Unconjugated (bs-1108R) at 1:200 overnight at 4˚C. Followed by secondary antibody (bs-0295G-HRP) incubation at 1:3000 for 90 min at 37˚C.
Lane 1: Human MOLT4 cell lysates; Lane 2: Human MCF-7 cell lysates probed with IGFBP2 Polyclonal Antibody, Unconjugated (bs-1108R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1108R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse tooth germ tissue labeled with Anti-Nanog Polyclonal Antibody (bs-0829R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Lane 1: A549 cell lysates probed with Rabbit Anti-Nanog Polyclonal Antibody, Unconjugated (bs-0829R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
HeLa cells probed with Estrogen Receptor alpha + beta Antibody, unconjugated (bs-0829R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)

Catalog #1: bs-0829R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded human kidney tissue labeled with Rabbit Anti- IFN-gamma \\nPolyclonal Antibody, Unconjugated (bs-0481R) at 1:200 followed by conjugation to the secondary \\nantibody and DAB staining
HL-60 lysates probed with IFN gamma Polyclonal Antibody, Unconjugated (bs-0481R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Molt-4 lysates probed with IFN gamma Polyclonal Antibody, Unconjugated (bs-0481R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-0481R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human gastric mucosa labeled with Anti-MMP-3 Polyclonal Antibody (bs-0413R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Lane 1: mouse spleen lysates Lane 2: mouse liver lysates probed with Anti MMP-3 Polyclonal Antibody, Unconjugated (bs-0413R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 42kD,52kDkD. Observed band size: 52kD.

Catalog #1: bs-0413R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Mouse brain lysates probed with MOG Polyclonal Antibody, unconjugated (bs-0426R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MOG Polyclonal Antibody, Unconjugated (bs-0426R) at 1:500 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Spinal cord tissue lysates; Lane 2: Rat Spinal cord tissue lysates probed with MOG Polyclonal Antibody, Unconjugated (bs-0426R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0426r

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0089R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Rabbit Anti-Nociceptin receptor Polyclonal Antibody (bs-0181R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human placenta tissue labeled with Rabbit Anti-Nociceptin receptor Polyclonal Antibody (bs-0181R) at 1:200 followed by conjugation to the secondary antibody and DAPI staining.
Formalin-fixed and paraffin embedded human placenta tissue labeled with Rabbit Anti-Nociceptin receptor Polyclonal Antibody (bs-0181R) at 1:200 followed by conjugation to the secondary antibody.
L1 mouse brain, L2 mouse kidney lysates probed (bs-0181R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band and observed band size: 40kDa.

Catalog #1: bs-0181R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-GLUT1 Polyclonal Antibody (bs-0472R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Image was kindly submitted by Dr. Lelisa Gemta from University of Virginia. Mouse splenocytes stained with Rabbit Anti-GLUT1 Polyclonal Antibody, PE conjugated (bs-0472R-PE)at 1:50.
Lane 1: Mouse Heart lysates; Lane 2: Mouse Cerebrum lysates; Lane 3: Mouse Muscle lysates; Lane 4: Rat Cerebrum lysates probed with GLUT1 Polyclonal Antibody, Unconjugated (bs-0472R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0472R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-8238R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat lung labeled with Anti-SPON1\/F spondin Polyclonal Antibody, Unconjugated (bs-7520R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Antigen: bs-7520P, 2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1: 5000; \\nTMB staining\\nRead the data in Microplate Reader by 450nm. \\n
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SPON1 Polyclonal Antibody, Unconjugated (bs-7520R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SPON1 Polyclonal Antibody, Unconjugated (bs-7520R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-7520R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti EphA7\/Eph receptor A7 Polyclonal Antibody, Unconjugated (bs-7034R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Rat brain lysates probed with Rabbit Anti-Eph receptor A7 Polyclonal Antibody, Unconjugated (bs-7034R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 107kD. Observed band size: 45kD
Lane 1: Mouse Hippocampus cell lysates probed with Eph receptor A7 Polyclonal Antibody, Unconjugated (bs-7034R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Rat Cerebrum cell lysates probed with Eph receptor A7 Polyclonal Antibody, Unconjugated (bs-7034R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-7034r

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat tongue labeled with Anti-Desmoglein 3\/DSG3 Polyclonal Antibody, Unconjugated (bs-6585R) at 1:200 followed by conjugation to the secondary antibody
Mouse tongue lysates probed with Desmoglein 3 Polyclonal Antibody, Unconjugated (bs-6585R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-6585R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-6318R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

RSC96 cells probed with Rabbit Anti-ADORA2B Polyclonal Antibody, PE Conjugated (bs-5900R-PE) at 1:30 for 40 minutes (green) compared to control cells (blue).
Mouse brain lysates probed with Rabbit Anti-ADORA2B Polyclonal Antibody (bs-5900R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ADORA2B Polyclonal Antibody, Unconjugated (bs-5900R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ADORA2B Polyclonal Antibody, Unconjugated (bs-5900R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-5900R

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti CD8 Polyclonal Antibody, Unconjugated (bs-4790R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CD8 Polyclonal Antibody, Unconjugated (bs-4790R) at 1:200 followed by secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (bs-0295G-Cy3) used at 1:200
U251 cells probed with CD8 Polyclonal Antibody, Unconjugated (bs-4790R) for 30 minutes followed by incubation with a conjugated secondary (FITC Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Catalog #1: bs-4790R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-4587R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Bovine, Pig, Rabbit

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti GABRG2\/GABA A Receptor gamma 2 Polyclonal Antibody, Unconjugated (bs-4112R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: human colon carcinoma lysates Lane 2: mouse brain lysates probed with Anti GABRG2\/GABA A Receptor gamma 2 Polyclonal Antibody, Unconjugated (bs-4112R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 51kD. Observed band size: 51kD.
Paraformaldehyde-fixed, paraffin embedded human glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GABA A Receptor gamma 2 Polyclonal Antibody, Unconjugated (bs-4112R) at 1:400 overnight at 4\u00b0C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
Lane 1: Mouse Cerebrum lysates; Lane 2: Rat Cerebrum lysates probed with GABA A Receptor gamma 2 Polyclonal Antibody, Unconjugated (bs-4112R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-4112R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat heart labeled with Anti Glucagon Receptor Polyclonal Antibody, Unconjugated (bs-3945R) followed by conjugation to the secondary antibody and DAB staining
Mouse kidney lysates probed with Glucagon Receptor Polyclonal Antibody, unconjugated (bs-3945R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human stomach tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Glucagon Receptor) Polyclonal Antibody, Unconjugated (bs-3945R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Glucagon Receptor Polyclonal Antibody, Unconjugated (bs-3945R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining.

Catalog #1: bs-3945R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Antigen: bs-2711P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Formalin-fixed and paraffin embedded mouse lymphoma labeled with Rabbit Anti IL-27R\/TCCR Polyclonal Antibody, Unconjugated (bs-2711R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse splenocytes probed with Rabbit Anti-IL-27RA Polyclonal Antibody, Unconjugated (bs-2711R) (green) at 1:100 for 40 minutes followed by a FITC conjugated secondary compared to control cells (blue).

Catalog #1: bs-2711R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney labeled with Rabbit Anti-GLIS3 Polyclonal Antibody, Unconjugated (bs-16249R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human kidney labeled with Rabbit Anti-GLIS3 Polyclonal Antibody, Unconjugated (bs-16249R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Human U251 cell lysates; Lane 2: Human U87MG cell lysates; Lane 3: Human A549 cell lysates; Lane 4: Human 293T cell lysates; Lane 5: Human MDA-MB-231 cell lysates probed with GLIS3 Polyclonal Antibody, Unconjugated (bs-16249R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-16249r

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat, Sheep

Conjugate: Unconjugated

Lane 1: Mouse spinal cord lysates probed with Anti \u2013ZNF312 Polyclonal Antibody, Unconjugated (bs-12148R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ZNF312 Polyclonal Antibody, Unconjugated (bs-12148R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Spinal cord tissue lysates; Lane 3: Rat Cerebrum tissue lysates probed with ZNF312 Polyclonal Antibody, Unconjugated (bs-12148R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-12148R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human rhinopolypus labeled with Rabbit Anti IL-23 Polyclonal Antibody, Unconjugated (bs-1193R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Ear tissue lysates; Lane 2: Human A431 cell lysates; Lane 3: Human Jurkat cell lysates probed with IL-23 Polyclonal Antibody, Unconjugated (bs-1193R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1193R

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-1121R

Applications: IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat pancreas tissue labeled with Anti-Tenascin C\/Tn-C Polyclonal Antibody, Unconjugated (bs-1039R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Formalin-fixed and paraffin embedded mouse stomach tissue labeled with Anti-Tenascin C\/Tn-C Polyclonal Antibody, Unconjugated (bs-1039R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n

Catalog #1: bs-1039R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-HGF Polyclonal Antibody, Unconjugated(bs-1025R) at 1:200, followed by conjugation to the secondary antibody and DAB staining\\n
Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HGF Polyclonal Antibody, Unconjugated (bs-1025R) at 1:400 overnight at 4\u00b0C, DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with HGF polyclonal antibody, unconjugated (bs-1025R) at 1:200 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bs-1025R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human rectal carcinoma labeled with Rabbit Anti ANGPTL1\/Angiopoietin 1\/ANG-1 Polyclonal Antibody, Unconjugated (bs-0800R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Angiopoietin-1) Polyclonal Antibody, Unconjugated (bs-0800R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Lane 1: Mouse Lung lysates; Lane 2: U87MG cell lysates; Lane 3: Mouse Heart lysates; Lane 4: Mouse Kidney lysates probed with Angiopoietin 1 Polyclonal Antibody, Unconjugated (bs-0800R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: K562 cell lysates; Lane 2: U87MG cell lysates; Lane 3: Hela cell lysates; Lane 4: Huvec cell lysates probed with Angiopoietin 1 Polyclonal Antibody, Unconjugated (bs-0800R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0800R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0294p-100ul

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-0466R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Sheep, Goat, Cow

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-Cytochrome C Polyclonal Antibody, Unconjugated (bs-0013R) 1:300 followed by conjugation to the secondary antibody and DAB staining
L1 mouse brain lysates L2 mouse lung lysates probed with Anti Cytochrome C Polyclonal Antibody, Unconjugated (bs-0013R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 26kD. Observed band size:26kD.
Image kindly submitted by an end-user via Funakoshi. 4% Paraformaldehyde fixed HeLa cells stained with Rabbit Anti-Cytochrome C Polyclonal Antibody (bs-0013R) at 1:100 for 2 hours at room temperature
HepG2 cells probed with Cytochrome C Polyclonal Antibody, unconjugated (bs-0013) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange).

Catalog #1: bs-0013R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-7678R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-6895R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: rat brain lysates probed with Rabbit Anti-RhoA(Ser188) Polyclonal Antibody, Unconjugated (bs-5330R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Molt-4 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with RhoA(Ser188) Polyclonal Antibody(bs-5330R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RhoA(Ser188) Polyclonal Antibody, Unconjugated (bs-5330R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-5330R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-4947R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Antigen: bs-4870P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Lane 1: mouse liver lysates Lane 2: mouse brain lysates probed with Anti MS4A2 Polyclonal Antibody, Unconjugated (bs-4870R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 27kD. Observed band size: 27kD.

Catalog #1: bs-4870R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Cyclin G2 Polyclonal Antibody, Unconjugated (bs-3822R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Rat brain lysates probed with Anti Cyclin G2 Polyclonal Antibody, Unconjugated (bs-3822R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 38kD. Observed band size:38kD.\\n

Catalog #1: bs-3822R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Antigen: Phospho Mu Opioid Receptor (blue line), 0.2ug\/100ul; Mu Opioid Receptor(red line), 0.2ug\/100ul\\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000; \\nTMB staining; \\nRead the data in MicroplateReader by 450nm. \\n
Image kindly provided by Dr. Stefen Clemens of East Carolina University. pMOR protein expression levels in the spinal cords of WT and D3KO mice (top lanes), and their respective \u00df-actin protein expression (bottom panels). Rabbit Anti-mu Opioid Receptor (Ser375) Polyclonal Antibody was used at a dilution of 1:1000.

Catalog #1: bs-3724R

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Phospho-Lyn (Tyr396) Polyclonal Antibody, Unconjugated (bs-3257R) followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse placenta lysates; Lane 2: Mouse lung lysates probed with Lyn (Tyr397) Polyclonal Antibody, Unconjugated (bs-3257R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:20000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Lyn (Tyr397) Polyclonal Antibody, Unconjugated (bs-3257R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat spleen tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Phospho-Lyn (Tyr397)) Polyclonal Antibody, Unconjugated (bs-3257R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-3257R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2615R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

293T cell lysate probed (bs-2075R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C. \\n
Paraformaldehyde-fixed, paraffin embedded rat lung tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-Heme Oxygenase Polyclonal Antibody, Unconjugated (bs-2075) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Lane 1: Human HepG2 cell lysates; Lane 2: Human A549 cell lysates; Lane 3: Human DU145 cell lysates; Lane 4: Human H9C2 cell lysates; Lane 5: Human 293T cell lysates probed with Heme Oxygenase Polyclonal Antibody, Unconjugated (bs-2075R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2075R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Unconjugated

Catalog #1: bs-1768R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-IDO2 Polyclonal Antibody, Unconjugated (bs-16640R) at 1:500 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: Mouse Placenta tissue lysates; Lane 2: Mouse Liver tissue lysates; Lane 3: Mouse Lung tissue lysates; Lane 4: Mouse Spleen tissue lysates ; Lane 5: Rat Liver tissue lysates ; Lane 6: Rat Spleen tissue lysates probed with INDOL1 Polyclonal Antibody, Unconjugated (bs-16640R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-16640R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

L1 rat lung lysates L2 rat liver lysates probed with Anti Hpt\/Haptoglobin Polyclonal Antibody, Unconjugated (bs-1808R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 44kD. Observed band size: 38kD,44kD
Lane 1: Hela cell lysates probed with Hpt\/Haptoglobin Polyclonal Antibody, Unconjugated (bs-1808R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1808R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-1411R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-ADAMTS1 Polyclonal Antibody, Unconjugated (bs-1208R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin-embedded rat tongue labeled with Anti-ADAMTS1 Polyclonal Antibody, Unconjugated(bs-1208R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Formalin-fixed and paraffin-embedded human colon carcinoma labeled with Anti-ADAMTS1 Polyclonal Antibody, Unconjugated(bs-1208R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Lane 1: Human Hela cell Lysates; Lane 2: Human 293T cell Lysates; Lane 3: Human U87-MG cell Lysates; Lane 4: Human U251 cell Lysates. Probed with ADAMTS1 polyclonal Antibody, unconjugated (bs-1208R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1208R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Mouse liver cells probed with Rabbit Anti-FLRT3 Polyclonal Antibody (bs-12363R) at 1:100 for 60 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary (bs-0295G-FITC) at 1:100 (green) for 40 minutes compared to control cells (blue)
Lane 1: Rat Cerebrum tissue lysates; Lane 2: Rat Lung tissue lysates probed with FLRT3 Polyclonal Antibody, Unconjugated (bs-12363R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-12363R

Applications: WB, ELISA

Reactivity: Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-5-HTR1A\/HTR1A Polyclonal Antibody, Unconjugated (bs-1124R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: Mouse Brain Lysates; Lane 2: Mouse Cerebellum Lysate; Lane 3: Mouse Lymph Lysates; Lane 4: Rat Brain Lysates; Lane 5: Rat Cerebellum Lysates. Probed with 5HT1A Receptor polyclonal Antibody, unconjugated (bs-1124R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1124R

Applications: WB, ELISA, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-Elastin Polyclonal Antibody, Unconjugated (bs-11057R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse lung lysates probed with Elastin Polyclonal Antibody, Unconjugated (bs-11057R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Lung lysates; Lane 2: Mouse Liver lysates; Lane 3: Rat Uterus lysates; Lane 4: Rat Liver lysates probed with Elastin Polyclonal Antibody, Unconjugated (bs-11057R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-11057R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated