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Western Blot Antibodies



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Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1: 2000\r\nPrimary ab incubation condition: 2 hours at\r\nroom temperature\r\nLysate: Mouse brain, Rat brain\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 15 s\r\nPredicted MW: 23 kDa\r\nObserved MW: 25 kDa
Tissue: Human cerebellum\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60238M
Tissue: Mouse cerebrall cortex\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60238M
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (SNAP25) Monoclonal Antibody, Unconjugated (bsm-60238M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-60238m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: room temperature 2h\r\nSecondary Ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: 1: MCF-7, 2: T47D, 3: MDA-MB-231\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 15 kDa\r\nObserved MW: 15 kDa\r\n
Cell line: HepG2\r\nFixative: 4% Paraformaldehydel\r\nPermeabilization: 0.1% TritonX-100\r\nPrimary Ab dilution: 1:50\r\nPrimary incubation condition: 4\u00b0C overnight\r\nSecondary Ab: Goat Anti-Mouse IgG\r\nNuclear counter stain: DAPI (Blue)\r\nComment: Color green is the positive signal for bsm-60551M
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (FIS1) monoclonal Antibody, Unconjugated (bsm-60551M) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.\u7f16\u53f7

Catalog #1: bsm-60551m

Applications: WB, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:10000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: 1: HeLa, 2: SH-SY5Y, 3: C2C12\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 3 s\r\nPredicted MW: 43 kDa\r\nObserved MW: 43 kDa\r\n
Tissue: Human liver cancer Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Mouse)(sp-0024) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60528M
Cell line: HeLa\r\nFixative: 4% Paraformaldehyde\r\nPermeabilization: 0.1% TritonX-100\r\nPrimary ab dilution: 1:50\r\nPrimary incubation condition: 4\u00b0C overnight\r\nSecondary ab: Goat Anti-Mouse IgG\r\nNuclear counter stain: DAPI (Blue)\r\nComment: Color green is the positive signal for bsm-60528M
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (WNT10B) Monoclonal Antibody, Unconjugated (bsm-60528M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-60528m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60239m

Applications: WB, IF

Reactivity: Mouse

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 4\u00b0C overnight\r\nSecondary Ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: 1: HeLa, 2: HEK-293, 3: Jurkat\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 54 kDa\r\nObserved MW: 70 kDa\r\n
Cell line: HepG2\r\nFixative: 4% Paraformaldehyde\r\nPermeabilization: 0.1% TritonX-100\r\nPrimary Ab dilution: 1:50\r\nPrimary incubation condition: 4\u00b0C overnight\r\nSecondary Ab: Goat Anti-Mouse IgG\r\nNuclear counter stain: DAPI (Blue)\r\nCounter stain: Tubulin (Red)\r\nComment: Color green is the positive signal for bsm-60400M
Lane 1: Human Hela cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human SH-SY5Y cell lysates; Lane 4: Human 293T cell lysates; Lane 5: Human Jurkat cell lysates probed with NRF1 monoclonal Antibody, Unconjugated (bsm-60400M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-60400m

Applications: WB, IF

Reactivity: Human

Conjugate: Unconjugated

SHSY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (CNPase) polyclonal Antibody, Unconjugated (bsm-60368M) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Rat Cerebrum tissue lysates; Lane 2: Rat Cerebellum tissue lysates probed with CNPase Monoclonal Antibody, Unconjugated (bsm-60368M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CNPase Monoclonal Antibody, Unconjugated(bsm-60368M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CNPase Monoclonal Antibody, Unconjugated(bsm-60368M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-60368m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Tissue: Human placenta Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab:SP Kit(Mouse)(sp-0024) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60352M
Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: room temperature 2h\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: JAR, HepG2\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 3 s\r\nPredicted MW: 52 kDa\r\nObserved MW: 52 kDa\r\n
Cell line: HepG2\r\nFixative: 100% Ice-cold methanol\r\nPermeabilization: 0.1% TritonX-100\r\nPrimary ab dilution: 1:50\r\nPrimary incubation condition: 4\u00b0C overnight\r\nSecondary ab: Goat Anti-Mouse IgG\r\nNuclear counter stain: DAPI (Blue)\r\nComment: Color green is the positive signal for bsm-60352M

Catalog #1: bsm-60352m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Catalase) Monoclonal Antibody, Unconjugated (bsm-60450M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Catalase) Monoclonal Antibody, Unconjugated (bsm-60450M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Catalase) Monoclonal Antibody, Unconjugated (bsm-60450M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Lane 1: Human HeLa cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human 293T cell lysates; Lane 4: Human Jurkat cell lysates; Lane 5: Human Raji cell lysates probed with Catalase Monoclonal Antibody, Unconjugated (bsm-60450M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-60450m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60045M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60058M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60056M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60063M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: (-) HeLa, (+) HeLa+Sodium butyrate (30mM, 4hr)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 14 kDa\r\nObserved MW: 14 kDa\r\n
Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:500\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: Mouse liver, Mouse spleen\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 14 kDa\r\nObserved MW: 14 kDa\r\n

Catalog #1: bsm-60062M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-60308m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: room temperature 2h\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: HeLa, MCF-7\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 28 kDa\r\nObserved MW: 28 kDa\r\n
Tissue: Human tonsil\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60449M
Tissue: esophagus\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60449M

Catalog #1: bsm-60449m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60270m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60165M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: 4\u00b0C overnight\r\nLysate: 1: HeLa, 2: MCF-7, 3: NIH\/3T3\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 161 kDa\r\nObserved MW: 161 kDa\r\n
Lane 1: Human HepG2 cell lysates; Lane 2: Human Hela cell lysates probed with ROCK2 Monoclonal Antibody, Unconjugated (bsm-60013M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-60013M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: HeLa, JAR\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 3 s\r\nPredicted MW: 45 kDa\r\nObserved MW: 110 kDa\r\n
Lane 1: Human HT-1080 cell Lysates; Lane 2: Human JAR cell Lysates; Lane 3: Human A549 cell Lysates; Lane 4: Human U-87 MG cell Lysates. Probed with LAMP1 monoclonal Antibody, unconjugated (bsm-60009M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Cell line: HeLa\r\nFixative: 100% Ice-cold methanol\r\nPermeabilization: 0.1% TritonX-100\r\nPrimary ab dilution: 1:50\r\nPrimary incubation condition: 4\u00b0C overnight\r\nSecondary ab: Goat Anti-Rabbit IgG\r\nNuclear counter stain: DAPI (Blue)\r\nComment: Color green is the positive signal for bsm-60009M

Catalog #1: bsm-60009M

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: 2 hours at\r\nroom temperature\r\nLysate: HepG2, Neuro-2a, EL4.IL-2\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 S\r\nPredicted MW: 86 kDa\r\nObserved MW: 86 kDa
Lane 1: Mouse NIH\/3T3 cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human HepG2 cell lysates probed with Mitofusin 2 Monoclonal Antibody, Unconjugated (bsm-60007M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-60007M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-60004M

Applications: WB

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33313m-fitc-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-33313m-Cy5.5-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-33313m-cy5-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33313m-cy3-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33313m-cy7-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-53023M

Applications: WB, ELISA, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51025R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51008R

Applications: WB

Reactivity: Human, Others

Conjugate: Unconjugated

Catalog #1: bs-51026R

Applications: WB, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51000R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51023R

Applications: WB, ELISA, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51024R

Applications: WB, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51022R

Applications: WB, ELISA, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51021R

Applications: WB, ELISA, IF, dot-blot

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51020R

Applications: WB, ELISA, dot-blot

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51019R

Applications: WB, ELISA, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51016R

Applications: WB, ELISA, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51015R

Applications: WB, ELISA, IF

Reactivity: Human, Others

Conjugate: Unconjugated

Catalog #1: bs-51014R

Applications: WB, ELISA, IF

Reactivity: Human, Others

Conjugate: Unconjugated

Catalog #1: bs-51013R

Applications: WB, ELISA, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51012R

Applications: WB, ELISA, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51004R

Applications: WB, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51003R

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-51007R

Applications: WB, IHC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33227M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33227m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-8772M

Applications: WB

Reactivity: Caenorhabditis Elegans

Conjugate: Unconjugated