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Western Blot Antibodies



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Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (HSC70) Monoclonal Antibody, Unconjugated (bsm-33211M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (HSC70) Monoclonal Antibody, Unconjugated (bsm-33211M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (HSC70) Monoclonal Antibody, Unconjugated (bsm-33211M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (HSC70) Monoclonal Antibody, Unconjugated (bsm-33211M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33211m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33413M-HRP

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig

Conjugate: HRP

Ctll-2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Bcl-2 monoclonal Antibody(bsm-33413M)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Ctll-2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Bcl-2 monoclonal Antibody(bsm-33413M)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bsm-33413M

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig

Conjugate: Unconjugated

Catalog #1: bsm-33412M-HRP

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig

Conjugate: HRP

Catalog #1: bsm-33412M-Biotin

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig

Conjugate: Biotin

Catalog #1: bsm-33412M

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig

Conjugate: Unconjugated

Catalog #1: bsm-33194M-HRP

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33194M-Biotin

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33194M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33102M-HRP

Applications: WB

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bsm-33102M-Biotin

Applications: WB

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bsm-33102M

Applications: WB, ICC/IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-33243m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-41197M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-41197M-HRP

Applications: WB

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-41197M-Biotin

Applications: WB

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-41196M-HRP

Applications: WB

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-41196M-Biotin

Applications: WB

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-41196M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-34016r-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-34016r-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FGF2 Monoclonal Antibody, Unconjugated(bsm-34016R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FGF2 Monoclonal Antibody, Unconjugated(bsm-34016R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FGF2 Monoclonal Antibody, Unconjugated(bsm-34016R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FGF2 Monoclonal Antibody, Unconjugated(bsm-34016R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-34016r-TR

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33245M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-33245M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-33243m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-33245M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Transferrin(Serum Loading Control) Monoclonal Antibody(bsm-33243M)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
25 ug total protein per lane of various lysates (see on figure) probed with Transferrin monoclonal antibody, unconjugated (bsm-33243M) at 1:5000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-33243m

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33235m-hrp-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: HRP

Catalog #1: bsm-33235m-biotin-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: Biotin

Catalog #1: bsm-33228m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33228m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33224M-HRP

Applications: WB

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-33224M

Applications: WB, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33221M-HRP

Applications: WB

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-33224M-Biotin

Applications: WB

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-33221M

Applications: WB, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33221M-Biotin

Applications: WB

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-33201M-HRP

Applications: WB

Reactivity: Goat, Cow

Conjugate: HRP

Catalog #1: bsm-33201M

Applications: WB

Reactivity: Goat, Cow

Conjugate: Unconjugated

Catalog #1: bsm-33198M-HRP

Applications: WB

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-33198M-Biotin

Applications: WB

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-33198M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33008m-biotin-100ul

Applications: WB

Reactivity: Species independent

Conjugate: Biotin

Catalog #1: bsm-0514M-HRP

Applications: WB

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-0514M-Biotin

Applications: WB

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-0514M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33278M

Applications: WB, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33441m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP