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Western Blot Antibodies



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Catalog #1: bsm-33053M-PE

Applications: WB

Reactivity: Human, Mouse

Conjugate: PE

Catalog #1: bsm-33049m-pe-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bsm-33053M-BF750

Applications: WB

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-750

Catalog #1: bsm-33049m-bf405-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33053M-HRP

Applications: WB

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bsm-33053M-BF350

Applications: WB

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33049m-pe-Cy5.5-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bsm-33053M-FITC

Applications: WB

Reactivity: Human, Mouse

Conjugate: FITC

Catalog #1: bsm-33053M-Cy5

Applications: WB

Reactivity: Human, Mouse

Conjugate: Cy5

Catalog #1: bsm-33053M-Cy3

Applications: WB

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #1: bsm-33049m-pe-cy5-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5

Lane 1: MCF-7 cell lysates; Lane 2: Hela cell lysates probed with AMACR Monoclonal Antibody, Unconjugated (bsm-33053M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (Human kidney carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (AMACR) Monoclonal Antibody, Unconjugated (bsm-33053M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Lane 1: Mouse Kidney tissue lysates; Lane 2: Mouse Liver tissue lysates probed with AMACR Monoclonal Antibody, Unconjugated (bsm-33053M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-33053m

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-33053M-BF488

Applications: WB

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33049m-apc-cy7-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bsm-33049m-fitc-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-33053M-Biotin

Applications: WB

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bsm-33053M-Cy7

Applications: WB

Reactivity: Human, Mouse

Conjugate: Cy7

Catalog #1: bsm-33053M-BF594

Applications: WB

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33049m-bf488-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33053M-BF555

Applications: WB

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33049m-cy5-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33049m-bf594-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33049m-cy3-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33049m-cy7-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-33049m-Cy5.5-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-33049m-bf555-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-555

4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Galectin 3) monoclonal Antibody, unconjugated (bsm-33049M) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
25 ug total protein per lane of various lysates (see on figure) probed with Galectin 3 monoclonal antibody, unconjugated (bsm-33049M) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with Galectin 3 monoclonal antibody, unconjugated (bsm-33049M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-33049m

Applications: WB, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33140m-apc-cy7-100ul

Applications: WB, IF(ICC)

Reactivity: Human

Conjugate: APC-Cy7

Catalog #1: bsm-33140m-apc-Cy5.5-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bsm-33140m-rbitc-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human

Conjugate: RBITC

Catalog #1: bsm-33140m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-33140m-apc-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human

Conjugate: APC

Catalog #1: bsm-33140m-pe-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-33140m-bf680-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bsm-33140m-cy7-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-33140m-percp-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human

Conjugate: PerCP

Catalog #1: bsm-33140m-pe-Cy5.5-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bsm-33140m-Cy5.5-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-33140m-bf594-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33140m-bf555-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33140m-fitc-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-33140m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-33140m-bf488-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33140m-pe-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bsm-33140m-cy3-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-33140m-pe-cy5-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5

Catalog #1: bsm-33140m-percp-Cy5.5-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-33140m-cy5-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human

Conjugate: Cy5