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Western Blot Antibodies



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Catalog #1: bs-9413R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Rat

Conjugate: Unconjugated

Antigen: bs-5490P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Mouse large intestine lysates probed with Ron (Tyr1238) Polyclonal Antibody, unconjugated (bs-5490R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.

Catalog #1: bs-5490R

Applications: WB, ELISA

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human breast cancer labeled with Rabbit Anti-AKT1\/2\/3 (Thr305\/308\/309) Polyclonal Antibody, Unconjugated (bs-5182R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-AKT1\/2\/3 (Thr305\/308\/309) Polyclonal Antibody, Unconjugated (bs-5182R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse lung lysates probed with Rabbit Anti-Akt1\/2\/3 (Thr305\/308\/309) Polyclonal Antibody, Unconjugated (bs-5182R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: HepG2 cell lysates probed with phospho-AKT1 + AKT2 + AKT3 (Thr308+Thr309+Thr305) Polyclonal Antibody, Unconjugated (bs-5182R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C. The upper band could be the fully glycosylated form of the target protein.

Catalog #1: bs-5182R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-4947R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-4236R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Cyclin G2 Polyclonal Antibody, Unconjugated (bs-3822R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Rat brain lysates probed with Anti Cyclin G2 Polyclonal Antibody, Unconjugated (bs-3822R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 38kD. Observed band size:38kD.\\n

Catalog #1: bs-3822R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Antigen: Phospho Mu Opioid Receptor (blue line), 0.2ug\/100ul; Mu Opioid Receptor(red line), 0.2ug\/100ul\\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000; \\nTMB staining; \\nRead the data in MicroplateReader by 450nm. \\n
Image kindly provided by Dr. Stefen Clemens of East Carolina University. pMOR protein expression levels in the spinal cords of WT and D3KO mice (top lanes), and their respective \u00df-actin protein expression (bottom panels). Rabbit Anti-mu Opioid Receptor (Ser375) Polyclonal Antibody was used at a dilution of 1:1000.

Catalog #1: bs-3724R

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-3667R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-2615R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat ileum labeled with Anti- MASP2 Polyclonal Antibody, Unconjugated (bs-1980R) at 1:200, followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat liver labeled with Anti- MASP2 Polyclonal Antibody, Unconjugated (bs-1980R) at 1:200, followed by conjugation to the secondary antibody and DAB staining.
Lane 1: mouse lung lysates Lane 2: mouse brain lysates probed with Rabbit Anti-MASP2 Polyclonal Antibody, Unconjugated (bs-1980R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 74kD. Observed band size: 74kD.
Paraformaldehyde-fixed, paraffin embedded; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with MASP2 Polyclonal Antibody, Unconjugated (bs-1980R)at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #1: bs-1980R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

L1 rat lung lysates L2 rat liver lysates probed with Anti Hpt\/Haptoglobin Polyclonal Antibody, Unconjugated (bs-1808R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 44kD. Observed band size: 38kD,44kD
Lane 1: Hela cell lysates probed with Hpt\/Haptoglobin Polyclonal Antibody, Unconjugated (bs-1808R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1808R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-Caveolin-1 Polyclonal Antibody, Unconjugated (bs-1453R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded mouse eye labeled with Anti Caveolin-1 Polyclonal Antibody, Unconjugated (bs-1453R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: mouse lung lysates Lane 2: mouse muscle lysates probed with Anti Caveolin-1 Polyclonal Antibody, Unconjugated (bs-1453R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 20kD. Observed band size: 20kD.
RSC96 cells probed with Caveolin-1 Polyclonal Antibody, Unconjugated (bs-1453R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Catalog #1: bs-1453R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-ADAMTS1 Polyclonal Antibody, Unconjugated (bs-1208R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin-embedded rat tongue labeled with Anti-ADAMTS1 Polyclonal Antibody, Unconjugated(bs-1208R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Formalin-fixed and paraffin-embedded human colon carcinoma labeled with Anti-ADAMTS1 Polyclonal Antibody, Unconjugated(bs-1208R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Lane 1: Human Hela cell Lysates; Lane 2: Human 293T cell Lysates; Lane 3: Human U87-MG cell Lysates; Lane 4: Human U251 cell Lysates. Probed with ADAMTS1 polyclonal Antibody, unconjugated (bs-1208R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1208R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Mouse liver cells probed with Rabbit Anti-FLRT3 Polyclonal Antibody (bs-12363R) at 1:100 for 60 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary (bs-0295G-FITC) at 1:100 (green) for 40 minutes compared to control cells (blue)
Lane 1: Rat Cerebrum tissue lysates; Lane 2: Rat Lung tissue lysates probed with FLRT3 Polyclonal Antibody, Unconjugated (bs-12363R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-12363R

Applications: WB, ELISA

Reactivity: Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human breast carcinoma labeled with Anti-CCR3 Polyclonal Antibody, Unconjugated (bs-1167R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat colitis labeled with Anti-CCR3 Polyclonal Antibody, Unconjugated (bs-1167R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Catalog #1: bs-1167R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-10251R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0784R

Applications: WB, ELISA, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue with labeled Anti-CD38 Polyclonal Antibody, Unconjugated (bs-0979R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Mouse splenocytes probed with CD38 Polyclonal Antibody, Unconjugated (bs-0979R) at 0.2ug for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Rat Cerebrum lysates probed with CD38 Polyclonal Antibody, Unconjugated (bs-0979R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0979R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse brain tissue labeled with Rabbit Anti-NF-H\/Neurofilament H\/Neurofilament 200 Polyclonal Antibody (bs-0708R), Unconjugated followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat spinal cord labeled with Rabbit Anti-NF-H\/Neurofilament H\/Neurofilament 200 Polyclonal Antibody (bs-0708R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat brain tissue labeled Anti-NF-H\/Neurofilament H\/Neurofilament 200 Polyclonal Antibody (bs-0708R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (NF-H) Polyclonal Antibody, Unconjugated (bs-0708R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-0708R

Applications: WB, ELISA, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney labeled with Anti-GAPDH Polyclonal Antibody, Unconjugated (bs-0459R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
This image was generously provided by Loyda M. Mel\u00e9ndez at the University of Puerto Rico Medical Sciences Campus.\\nA total of 30ug of monocyte-derived macrophage lysates were reduced and denatured in 1X Laemli Buffer with beta-mercaptoethanol. Samples were loaded into a TGX 4-20% gel, at 150V for 1 hour. Proteins were transferred to a PVDF membrane and probed for proteins of interest. The membrane was re-probed with Rabbit Anti-GAPDH Polyclonal Antibody (bs-0459R) in 5% milk at 1:250 dilution overnight at 4\u00b0C. The next day, the membrane was washed with TBS-T and incubated with goat anti-rabbit HRP conjugated secondary antibody for 1 hour at room temperature. The membrane was exposed for 18 seconds.\\n
MCF-7 cell lysates probed with Rabbit Anti-GAPDH Polyclonal Antibody (bs-0459R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP ) at 1:5000 for 90 min at 37˚C.
Mouse muscle lysates probed with Rabbit Anti-GAPDH Polyclonal Antibody (bs-0459R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP ) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-0459R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat colon tissue labeled with Anti-Fas Polyclonal Antibody, Unconjugated (bs-0215R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Thymus tissue lysates; Lane 2: Rat Thymus tissue lysates; Lane 3: Human HepG2 cell Lysates; Lane 4: Human Raji cell Lysates; Lane 5: Human HT1080 cell Lysates; Lane 6: Human A431 cell Lysates probed with CD95\/FAS Polyclonal Antibody, Unconjugated (bs-0215R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.\r\n

Catalog #1: bs-0215R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney tissue labeled withAnti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032r) 1:400 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat liver tissue labeled with Anti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032R) 1:400 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat myocardium tissue labeled with Anti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032R) 1:300 followed by conjugation to the secondary antibody and DAB staining
L1 mouse embryo lysates, L2 mouse brain lysates probed with Anti- Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. P

Catalog #1: bs-0032r

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1004R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: FITC

Mouse heart lysates probed with LCLAT1 Polyclonal Antibody, Unconjugated (bs-18190R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Heart lysates; Lane 2: Rat Heart lysates probed with LCLAT1 Polyclonal Antibody, Unconjugated (bs-18190R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-18190R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-8406R

Applications: WB, ELISA

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat skin tissue labeled with Anti-S100A7 Polyclonal Antibody, Unconjugated(bs-6575R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
K562 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with S100A7 Antibody(bs-6575R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-6575r

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-6217R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-6106R

Applications: WB, ELISA, IHC-P

Reactivity: Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with WNT4 Polyclonal Antibody, Unconjugated (bs-6134R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with WNT4 Polyclonal Antibody, Unconjugated (bs-6134R) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Kidney lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human A431 cell lysates; Lane 4: Human U-2OS cell lysates probed with WNT4 Polyclonal Antibody, Unconjugated (bs-6134R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-6134R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Antigen: bs-2967P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat Anti-Rabbit IgG (bs-0295G-HRP) at 1: 5000; \\nTMB staining; Read the data in MicroplateReader by 450nm.
Lane 1: MBP-tagged fusion protein Overexpression E.coli Lysate (Cat#: bs-33131P) probed with Maltose Binding Protein\/MBP Polyclonal Antibody, Unconjugated (bs-2967R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: MBP-Tagged Fusion Protein Overexpression E.coli Lysate (bs- 41233P) probed with MBP tag Polyclonal Antibody, Unconjugated (bs-2967R) at 1:200000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2967R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Species independent

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human oral squamous cell carcinoma labeled with Anti- MUC4 Polyclonal Antibody, Unconjugated (bs-1994R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: PANC1; Lane 2: LOVO; Lane 3: mouse thymus; Lane 4: MKN45 lysates probed with Mucin 4 Polyclonal Antibody, Unconjugated (bs-1994R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C

Catalog #1: bs-1994R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-P53(wt-p53) Polyclonal Antibody, Unconjugated (bs-2090R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
MCF7 cells were fixed with 70% ice-cold methanol overnight at 4\u2103, permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with P53(wt-p53) Polyclonal Antibody at 1:50 dilution in blocking buffer and incubated for 30 min at -20\u2103, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed.
Lane 1: Mouse Liver lysates; Lane 2: Rat Liver lysates probed with P53(wt-p53) Polyclonal Antibody, Unconjugated (bs-2090R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Tissue\/cell: A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (P53(wt-p53)) polyclonal Antibody, Unconjugated (bs-2090R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-2090r

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-ANP Polyclonal Antibody, Unconjugated (bs-2040R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Lane 1: Heart (Mouse); Lane 2: Heart (Rat) lysates probed with ANP Polyclonal Antibody, Unconjugated (bs-2040R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C

Catalog #1: bs-2040R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1432r

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Hela cells probed with Giantin\/GM130 Polyclonal Antibody, Unconjugated (bs-13356R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Giantin\/GOLGB1 Polyclonal Antibody Polyclonal Antibody, Unconjugated (bs-13356R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human cervix cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with\\tGiantin\/GOLGB1 Polyclonal Antibody Polyclonal Antibody, Unconjugated (bs-13356R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Giantin Polyclonal Antibody, Unconjugated (bs-13356R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Catalog #1: bs-13356R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human placenta labeled with Anti-ABCG1 Polyclonal Antibody, Unconjugated (bs-1231R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Molt4 cells(black) were fixed with 4% PFA for 10min at room temperature, permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ABCG1 Polyclonal Antibody, Cy5.5 Conjugated (bs-1231R-Cy5.5) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
Paraformaldehyde-fixed, paraffin-embedded Rat Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 20 minutes; Antibody incubation with ABCG1 Polyclonal Antibody, Unconjugated (bs-1231R) at 1:400 overnight at 4\u00b0C, followed by incubation with a conjugated secondary antibody, DAB and counterstaining.
Paraformaldehyde-fixed, paraffin-embedded Mouse Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 20 minutes; Antibody incubation with ABCG1 Polyclonal Antibody, Unconjugated (bs-1231R) at 1:400 overnight at 4\u00b0C, followed by incubation with a conjugated secondary antibody, DAB and counterstaining.

Catalog #1: bs-1231R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat small intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Progestin Receptor Beta Polyclonal Antibody, Unconjugated (bs-11410R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Progestin Receptor Beta\/MPRB Antibody(bs-11410R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Progestin Receptor Beta\/MPRB Antibody (bs-11410R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
Lane 1: Mouse Hippocampus lysates; Lane 2: Mouse Medulld lysates; Lane 3: Rat Hippocampus lysates; Lane 4: Rat Medulld lysates probed with Progestin Receptor Beta\/MPRB Polyclonal Antibody, Unconjugated (bs-11410R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-11410R

Applications: WB, ELISA

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: Serum (Mouse) at 30 ug\r\nLane 2: Plasma (Mouse) at 30 ug\r\nLane 3: Liver (Mouse) Tissue Lysate at 40 ug\r\nLane 4: Cerebrum (Mouse) Tissue Lysate at 40 ug\r\nLane 5: Spleen (Mouse) Tissue Lysate at 40 ug\r\nPrimary: Mouse IgA (bs-0774R-HRP) at 1\/1000 dilution\r\nSecondary: Goat Anti-Rabbit IgG at 1\/20000 dilution\r\nPredicted band size: 55 kD\r\nObserved band size: 62 kD
Lane 1: Serum (Mouse) at 40 ug\r\nLane 2: Small intestine (Mouse) Lysate at 40 ug\r\nLane 3: Lung (Mouse) Lysate at 40 ug\r\nPrimary: Mouse IgA (bs-0774R) at 1\/1000 dilution\r\nSecondary: Goat Anti-Rabbit IgG at 1\/20000 dilution\r\nPredicted band size: 55 kD\r\nObserved band size: 60 kD

Catalog #1: bs-0774r

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human gastric tissue labeled with Anti-Leptin Polyclonal Antibody (bs-0409R), Unconjugated at 1:300, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat Brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Leptin Polyclonal Antibody, Unconjugated (bs-0409R) at 1:400 overnight at 4\u00b0C, DAB staining.
Leptin lysates probed with Leptin Polyclonal Antibody, Unconjugated (bs-0409R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Leptin Polyclonal Antibody, Unconjugated (bs-0409R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0409R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0156R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-Bio 1:1000) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-Bio 1:500) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (RAT brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-Bio 1:1000) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-Bio 1:2000) instructionsand DAB staining.

Catalog #1: bs-0295G-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Rabbit

Conjugate: Biotin

Catalog #1: bs-0088R

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0522R-BF555

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-555

Lane 1: U937 cell lysates probed with p16-INK4a Polyclonal Antibody, Unconjugated (bs-23797R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CDKN2A\/p16-INK4a Polyclonal Antibody, Unconjugated (bs-23797R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CDKN2A\/p16-INK4a Polyclonal Antibody, Unconjugated (bs-23797R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human breast cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CDKN2A\/p16-INK4a) Polyclonal Antibody, Unconjugated (bs-23797R ) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-23797R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

IF(ICC) staining with SCGB1A1 (7G5) Monoclonal Antibody (bsm-54221R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with SCGB1A1 (7G5) Monoclonal Antibody (bsm-54221R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with SCGB1A1 (7G5) Monoclonal Antibody (bsm-54221R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Flow cytometric analysis of A549 cells with SCGB1A1 (7G5) Monoclonal Antibody (bsm-54221R) 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

Catalog #1: bsm-54221r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Flow cytometric analysis of HeLa cells with Alkaline Phosphatase (1G9 ) Monoclonal Antibody (bsm-52252R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; blue).
IF(ICC) staining with Alkaline Phosphatase (1G9) Monoclonal Antibody (bsm-52252R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Alkaline Phosphatase (1G9) Monoclonal Antibody (bsm-52252R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Alkaline Phosphatase (1G9) Monoclonal Antibody (bsm-52252R) at 1:100 in SW480 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52252r

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

MCF7 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with HSF1 (S326) (37E4) Monoclonal Antibody(bsm-52166R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed and paraffin-embedded Mouse lung tissue incubated with HSF1 (S326) (37E4) Monoclonal Antibody (bsm-52166R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with HSF1 (S326) (37E4) Monoclonal Antibody (bsm-52166R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse spleen tissue incubated with HSF1 (S326) (37E4) Monoclonal Antibody (bsm-52166R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52166r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-0107m

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Rat

Conjugate: Unconjugated