Bioss is dedicated to helping you achieve exceptional results. Our detailed listings for reagnets and buffers for your application can be found below.

Western Blotting Buffers

RIPA Buffer

• 150mM NaCl
• 1.0% NP-40 or 0.1% Triton X-100
• 0.5% sodium deoxycholate
• 0.1% SDS (sodium dodecyl sulphate)
• 50mM Tris-HCl pH8.0
• Protease Inhibitors

Tris-HCl buffer

• 20mM Tris-HCl pH7.5
• Protease Inhibitors

Denaturing lysis buffer

• 1% SDS
• 5mM EDTA
• Immediately before use add:
  • 10mM dithiothreitol or beta-mercaptoethanol
  • Protease inhibitors
  • 15U/mL DNase1

Laemmli 2X buffer/loading buffer

• 4%SDS
• 10% 2-mercaptoethanol
• 20% glycerol
• 0.004% bromophenol blue
• 0.125M Tris-HCl
• Check the pH and adjust pH to 6.8.

Running buffer

• 25mM Tris base
• 190mM glycine
• 0.1% SDS
• Check the pH, which should be about pH 8.3. Adjust if necessary.

Transfer buffer (wet)

• 25mM Tris base
• 190mM glycine
• 0.1% SDS
• The pH should be about pH8.3. Adjust if necessary.

Transfer buffer (semi-dry)

• 48mM Tris
• 39mM glycine
• 20% methanol
• 0.04% SDS

Blocking buffer

• 5% milk or BSA(bovine serum albumin)
• Add to TBST buffer. Mix well and filter.

Immunohistochemistry/Immunocytochemistry Buffers

Formalin solution (10%)

• 3.7-4% Formaldehyde (37-40%)
• 33mM NaH₂PO₄
• 46mM Na₂HPO₄

Paraformaldehyde (4%)

• 8% paraformaldehyde

0.2M Phosphate Buffer(PB) pH7.4

• 53mM NaH₂PO₄
• 154mM Na₂HPO₄
• Heat 8%PFA solution at 60C while stirring. Once the solution reaches 60C and the PFA dissolves, add 500mL of 0.2M phosphate buffer, to bring the solution to 4%PFA in 0.1M phosphate. Carefully add 1N NaOH until the solution is clear. Cool the solution and filter.

Sodium Citrate Buffer pH6.0

• 10mM sodium citrate
• 0.05% Tween20
• Mix to dissolve sodium citrate and adjust pH to 6.0 with 1N HCl.
• Add Tween20 and mix well.
• Store at room temperature for 3 months or at 4C for longer storage.

ELISA Buffers

Bicarbonate/carbonate coating buffer (1oomM) pH9.6

• Antigen or antibody should be diluted in coating buffer to immobilize them to the wells:
• 29mM Na₂CO₃
• 71mM NaHCO₃

Blocking solution

• Commonly used blocking agents are 1% BSA, serum, or non-fat dry milk in PBS.

Wash solution

• Usually PBS or Tris-buffered saline(pH7.4) with detergent such as 0.05% (v/v) Tween 20 (TBST).

Flow Cytometry

FACS buffer/antibody dilution buffer

• 10% FCS
• 1% sodium azide in PBS

Permeabilization

• 0.1-1% Triton X-100/NP-40 in PBS

Fixative

• 0.01-0.1% paraformaldehyde in PBS.