inclusion bodies and refolding

What can you do when non-specific staining in your IHC is obscuring your results and incurring your PI’s disdain?

Polyacrylamide is a common reagent in the molecular biology lab as it is used in the formation of gels used to study proteins in techniques such as Western blotting and polyacrylamide gel electrophoresis (PAGE).

Non-specific bands and high background on westerns can be a major source of frustration at the bench. The tips below are designed to help clean up background on your blots.

Learn the process of double staining for immunofluorescence and the advantages and disadvantages of using different antibodies for the procedure

Learn the differences between fluorescent and chromogenic detection techniques for analyzing immunohistochemistry (IHC) experiments