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Primary Antibodies

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Lane 1: 293 Cells; Lane 2: Mouse Brain; Lane 3: Rat Brain; Probed with KHSRP (2F11) Monoclonal Antibody (bsm-54455R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with KHSRP (2F11) Monoclonal Antibody (bsm-54455R) at 1:100 in 293T cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with KHSRP (2F11) Monoclonal Antibody (bsm-54455R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with KHSRP (2F11) Monoclonal Antibody (bsm-54455R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-54455R

Applications: WB, IHC-P, IF(IHC-P), IF(ICC), IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: U937 Cells; Probed with FYB1 (1F10) Monoclonal Antibody (bsm-54461R) at 1:500, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with FYB1 (1F10) Monoclonal Antibody (bsm-54461R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.
Paraformaldehyde-fixed and paraffin-embedded Human Appendix tissue incubated with FYB1 (1F10) Monoclonal Antibody (bsm-54461R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.
Paraformaldehyde-fixed and paraffin-embedded Human Spleen tissue incubated with FYB1 (1F10) Monoclonal Antibody (bsm-54461R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.

Catalog #1: bsm-54461R

Applications: WB, FCM, IHC-P, IHC

Reactivity: Human

Conjugate: Unconjugated

Lane 1: HepG2 Cells; Lane 2: MCF-7 Cells; Probed with SRP54 (1A4) Monoclonal Antibody (bsm-54457R) at 1:500, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with SRP54 (1A4) Monoclonal Antibody (bsm-54457R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
Paraformaldehyde-fixed and paraffin-embedded Human Breast Cancer tissue incubated with SRP54 (1A4) Monoclonal Antibody (bsm-54457R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with SRP54 (1A4) Monoclonal Antibody (bsm-54457R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

Catalog #1: bsm-54457R

Applications: WB, FCM, IHC-P, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of Daudi cells with CD79a (1B12) Monoclonal Antibody (bsm-54458R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Paraformaldehyde-fixed and paraffin-embedded Human Spleen tissue incubated with CD79a (1B12) Monoclonal Antibody (bsm-54458R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.
Paraformaldehyde-fixed and paraffin-embedded Human Appendix tissue incubated with CD79a (1B12) Monoclonal Antibody (bsm-54458R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.
Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with CD79a (1B12) Monoclonal Antibody (bsm-54458R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.

Catalog #1: bsm-54458R

Applications: WB, FCM, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Lane 1: SH-SY5Y Cells; Probed with TBXT (1F11) Monoclonal Antibody (bsm-54459R) at 1:500, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Mouse Embryo tissue incubated with TBXT (1F11) Monoclonal Antibody (bsm-54459R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
Flow cytometric analysis of A549 cells with TBXT (1F11) Monoclonal Antibody (bsm-54459R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

Catalog #1: bsm-54459R

Applications: WB, FCM, IHC-P, IF(ICC), IP

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: Rat Heart; Probed with NDUFS4 (2F3) Monoclonal Antibody (bsm-54460R) at 1:500, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Rat Uterus tissue incubated with NDUFS4 (2F3) Monoclonal Antibody (bsm-54460R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.
Paraformaldehyde-fixed and paraffin-embedded Human Breast Cancer tissue incubated with NDUFS4 (2F3) Monoclonal Antibody (bsm-54460R) at 1:100, 30 min at room temperature, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.
Paraformaldehyde-fixed and paraffin-embedded Human Small Intestine tissue incubated with NDUFS4 (2F3) Monoclonal Antibody (bsm-54460R) at 1:100, 30 min at room temperature, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.

Catalog #1: bsm-54460R

Applications: WB, FCM, IHC-P, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse testis; Lane 2: Rat brain; Lane 3: A431 Cells; Lane 4: SH-SY-5Y Cells; Lane 5: SiHa Cells; Lane 6: 293 Cells; Lane 7: Hela Cells; Lane 8: HepG2 Cells; Lane 9: A549 Cells; Probed with SFRS3 (9C11) Monoclonal Antibody (bsm-54467R) at 1:500, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with SFRS3 (9C11) Monoclonal Antibody (bsm-54467R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.
Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with SFRS3 (9C11) Monoclonal Antibody (bsm-54467R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.
Paraformaldehyde-fixed and paraffin-embedded Human Breast Cancer tissue incubated with SFRS3 (9C11) Monoclonal Antibody (bsm-54467R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.

Catalog #1: bsm-54468R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Rat Brain; Lane 2: A431 Cells; Lane 3: 293 Cells; Probed with MARK3 (2F7) Monoclonal Antibody (bsm-54462R) at 1:500, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with MARK3 (2F7) Monoclonal Antibody (bsm-54462R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.1% Triton X100\/TBS for 10 mins at room temperature.
IF(ICC) staining with MARK3 (2F7) Monoclonal Antibody (bsm-54462R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.1% Triton X100\/TBS for 10 mins at room temperature.
IF(ICC) staining with MARK3 (2F7) Monoclonal Antibody (bsm-54462R) at 1:100 in SiHa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.1% Triton X100\/TBS for 10 mins at room temperature.

Catalog #1: bsm-54462R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC), IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse skeletal muscle; Lane 2: Rat brain; Lane 3: A431 Cells; Lane 4: SH-SY5Y Cells; Lane 5: SiHa Cells; Lane 6: 293 Cells; Lane 7: Hela Cells; Lane 8: PC-3M Cells; Lane 9: HepG2 Cells; Lane 10: A549 Cells; Probed with PPP1CB (6G6) Monoclonal Antibody (bsm-54463R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with PPP1CB (6G6) Monoclonal Antibody (bsm-54463R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
Paraformaldehyde-fixed and paraffin-embedded Human Appendix tissue incubated with PPP1CB (6G6) Monoclonal Antibody (bsm-54463R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
Paraformaldehyde-fixed and paraffin-embedded Human Esophagus tissue incubated with PPP1CB (6G6) Monoclonal Antibody (bsm-54463R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

Catalog #1: bsm-54463R

Applications: WB, IHC-P, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse Heart; Probed with ACTN2 (7G3) Monoclonal Antibody (bsm-54465R) at 1:500, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Rat Heart tissue incubated with ACTN2 (7G3) Monoclonal Antibody (bsm-54465R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.
Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with ACTN2 (7G3) Monoclonal Antibody (bsm-54465R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.
Paraformaldehyde-fixed and paraffin-embedded Human Fetal Skeletal Muscle tissue incubated with ACTN2 (7G3) Monoclonal Antibody (bsm-54465R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.

Catalog #1: bsm-54465R

Applications: WB, IHC-P, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Human Fetal Skeletal Muscle; Probed with ATP2A1 (3G1) Monoclonal Antibody (bsm-54466R) at 1:500, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human Fetal Skeletal Muscle tissue incubated with ATP2A1 (3G1) Monoclonal Antibody (bsm-54466R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

Catalog #1: bsm-54466R

Applications: WB, IHC-P, IF(ICC), IP

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Mouse testis; Lane 2: A431 Cells; Lane 3: SH-SY5Y Cells; Lane 4: SiHa Cells; Lane 5: 293 Cells; Lane 6: Hela Cells; Lane 7: HepG2 Cells; Probed with RPA2 (4F8) Monoclonal Antibody (bsm-54464R) at 1:500, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with RPA2 (4F8) Monoclonal Antibody (bsm-54464R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.
Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with RPA2 (4F8) Monoclonal Antibody (bsm-54464R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.
Paraformaldehyde-fixed and paraffin-embedded Human Breast Cancer tissue incubated with RPA2 (4F8) Monoclonal Antibody (bsm-54464R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.

Catalog #1: bsm-54464R

Applications: WB, IHC-P, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse testis; Lane 2: Rat brain; Lane 3: A431 Cells; Lane 4: SH-SY-5Y Cells; Lane 5: SiHa Cells; Lane 6: 293 Cells; Lane 7: Hela Cells; Lane 8: HepG2 Cells; Lane 9: A549 Cells; Probed with RNF40 (8D8) Monoclonal Antibody (bsm-54467R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with RNF40 (8D8) Monoclonal Antibody (bsm-54467R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

Catalog #1: bsm-54467R

Applications: WB, IHC-P, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Jurkat cell lysates probed with ERG (3F9) Monoclonal Antibody, unconjugated (bsm-52324R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.\r\n
Paraformaldehyde-fixed and paraffin-embedded Mouse heart tissue incubated with ERG (3F9) Monoclonal Antibody (bsm-52324R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with ERG (3F9) Monoclonal Antibody (bsm-52324R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52324R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Raji cell lysates; Lane 2: Jurkat cell lysates probed with CD86 (4G10) Monoclonal Antibody (bsm-52375R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.
IF(ICC) staining with CD86 (4G10) Monoclonal Antibody (bsm-52375R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with CD86 (4G10) Monoclonal Antibody (bsm-52375R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with CD86 (4G10) Monoclonal Antibody (bsm-52375R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52375R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Rat

Conjugate: Unconjugated

Flow cytometric analysis of SH-SY5Y cells with NDUFS4 (5A5) Monoclonal Antibody (bsm-54436R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).
Paraformaldehyde-fixed and paraffin-embedded Human Small Intestine tissue incubated with NDUFS4 (5A5) Monoclonal Antibody (bsm-54436R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Rat Kidney tissue incubated with NDUFS4 (5A5) Monoclonal Antibody (bsm-54436R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Lane 1: Rat Heart; Probed with NDUFS4 (5A5) Monoclonal Antibody (bsm-54436R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-54436R

Applications: WB, FCM, IHC-P, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse lymph node; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD27 (3C3) Monoclonal Antibody, Unconjugated (bsm-54318R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD27 (3C3) Monoclonal Antibody, Unconjugated (bsm-54318R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Spleen lysates; Lane 2: Mouse Lymph node lysates; Lane 3: Mouse Thymus lysates; Lane 4: Mouse Plasma lysates probed with CD27 (3C3) Monoclonal Antibody, Unconjugated (bsm-54318R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-54318R

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of Jurkat cells with gamma Tubulin (8C1) Monoclonal Antibody (bsm-52814R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: A431 lysates; Lane 2: Jurkat lysates; Line 3: Mouse brain lysates; Probed with gamma Tubulin (8C1) Monoclonal Antibody (bsm-52814R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with gamma Tubulin (8C1) Monoclonal Antibody (bsm-52814R) at 1:100 in SHG-44 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with gamma Tubulin (8C1) Monoclonal Antibody (bsm-52814R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52814R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: HepG2 treated with Etoposide 20uM and Trichostatin A 500 nM for 6 hours, whole cell lysates (positive control); Lane 2: untreated HepG2, whole cell lysates; probed with p53(acetyl K370) (2F1) Monoclonal Antibody, Unconjugated (bsm-52288R) at 1:1000 overnight at 4\u00b0C followed by incubation with a conjugated secondary antibody at 1:20000 for 60 minutes at 37\u00b0C.
IF(ICC) staining with p53(acetyl K370) (2F1) Monoclonal Antibody (bsm-52288R) at 1:100 in PANC-1 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with p53(acetyl K370) (2F1) Monoclonal Antibody (bsm-52288R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52288R

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Hela; Lane 2: NIH\/3T3 lysate probed with Histone H3(mono methyl K36) (6G6) Monoclonal Antibody, Unconjugated (bsm-52298R) at 1:1000 overnight at 4\u00b0C followed by incubation with a conjugated secondary antibody at 1:20000 for 60 minutes at 37\u00b0C.
IF(ICC) staining with Histone H3(mono methyl K36) (6G6) Monoclonal Antibody (bsm-52298R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52298R

Applications: WB, IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: Untreated HeLa cell lysate; Lane 2: HeLa cell lysate treated with calyculin A; Lane 3: HeLa cell lysate treated with calyculin A and alkaline phosphatase; Phospho-Histone H3 (S10) (2F6) Monoclonal Antibody (bsm-52261R) at 1:1000 dilution, Histone H3 antibody and GAPDH antibody as control, incubated overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.\r\n
IF(ICC) staining with Phospho-Histone H3 (Ser10) (2F6) Monoclonal Antibody (bsm-52261R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Phospho-Histone H3 (Ser10) (2F6) Monoclonal Antibody (bsm-52261R) at 1:100 in AGS cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Phospho-Histone H3 (Ser10) (2F6) Monoclonal Antibody (bsm-52261R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52261R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Hela; Lane 2: HepG2; Probed with CDT1 (2H5) Monoclonal Antibody (bsm-54038R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Histone H3 (mono methyl K18) (2H4) Monoclonal Antibody (bsm-52244R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Histone H3 (mono methyl K18) (2H4) Monoclonal Antibody (bsm-52244R) at 1:100 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Histone H3 (mono methyl K18) (2H4) Monoclonal Antibody (bsm-52244R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52244R

Applications: WB, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Untreated CRC whole cell lysates; Lane 2: CRC cells treated with 1.5\u00b5g\/ml Colcemid for 12 hours whole cell lysates; Probed with Phospho-Histone H1.3 (T17)+Histone H1.4 (T17) (3A4 ) Monoclonal Antibody (bsm-52267R) at 1:500, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Phospho-Histone H1.3 (T17)+Histone H1.4 (T17) (3A4) Monoclonal Antibody (bsm-52267R) at 1:100 in NIH\/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse colon cancer tissue incubated with Phospho-Histone H1.3 (T17)+Histone H1.4 (T17) (3A4) Monoclonal Antibody (bsm-52267R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52267R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: HeLa treated with 500 ng\/ml Trichostatin A for 4 hours whole cell lysates; Lane 2: Untreated HeLa whole cell lysates probed with Histone H2A(acetyl K9) (4G2) Monoclonal Antibody (bsm-52285R) at 1:500 and unmodified Histone H2A.X antibody as control, incubated overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Histone H2A (acetyl K9) (4G2) Monoclonal Antibody (bsm-52285R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Histone H2A (acetyl K9) (4G2) Monoclonal Antibody (bsm-52285R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Histone H2A (acetyl K9) (4G2) Monoclonal Antibody (bsm-52285R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52285R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse testis; Lane 2: CRC lysate probed with Histone H3(mono+di+methyl K79) (2B11) Monoclonal Antibody (bsm-52291R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with Histone H3 (mono+di+methyl K79) (2B11) Monoclonal Antibody (bsm-52291R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human colon cancer tissue incubated with Histone H3 (mono+di+methyl K79) (2B11) Monoclonal Antibody (bsm-52291R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52291R

Applications: WB, FCM, IHC-P, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Hela; Lane 2: NIH\/3T3 lysate probed with Histone H2A(hydroxyl Y39) (2A3) Monoclonal Antibody (bsm-52286R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with Histone H2A (hydroxyl Y39) (2A3) Monoclonal Antibody (bsm-52286R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human colon cancer tissue incubated with Histone H2A (hydroxyl Y39) (2A3) Monoclonal Antibody (bsm-52286R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with Histone H2A (hydroxyl Y39) (2A3) Monoclonal Antibody (bsm-52286R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52286R

Applications: WB, IHC-P, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Hela cells; Lane 2: NIH\/3T3 cells; Lane 3: SH-SY-5Y cells; Probed with Phospho-PAK1 (S144)+PAK2 (S141)+PAK3 (S139) (9A3) Monoclonal Antibody (bsm-52435R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Flow cytometric analysis of nih-3t3 cells with Phospho-PAK1 (S144)+PAK2 (S141)+PAK3 (S139) (9A3) Monoclonal Antibody (bsm-52435R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with Phospho-PAK1 (S144)+PAK2 (S141)+PAK3 (S139) (9A3) Monoclonal Antibody (bsm-52435R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human liver cancer tissue incubated with Phospho-PAK1 (S144)+PAK2 (S141)+PAK3 (S139) (9A3) Monoclonal Antibody (bsm-52435R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52435R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-52578R

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse Brain lysate probed with Tau (Thr231) (13D4) Monoclonal Antibody (bsm-52507R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with Phospho-Tau (T231) (13D4) Monoclonal Antibody (bsm-52507R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52507R

Applications: WB, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse eyeball lysate probed with Phospho-PKC zeta (Thr560) (26G8) Monoclonal Antibody (bsm-52632R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with PKC zeta (Thr560) (26G8) Monoclonal Antibody (bsm-52632R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with PKC zeta (Thr560) (26G8) Monoclonal Antibody (bsm-52632R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52632R

Applications: WB, IHC-P, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-52579R

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Rat Liver; Lane 2: Mouse Lung; Probed with Histone H3 (acetyl K14) (1C6) Monoclonal Antibody (bsm-54178R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Jurkat Cells; Probed with Histone H3 (acetyl K14) (1C6) Monoclonal Antibody (bsm-54178R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Histone H3 (acetyl K14) (1C6) Monoclonal Antibody (bsm-54178R) at 1:200 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Histone H3 (acetyl K14) (1C6) Monoclonal Antibody (bsm-54178R) at 1:200 in MCF-7cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-54178R

Applications: WB, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

IF(ICC) staining with ATM (Ser1981) (3F10) Monoclonal Antibody (bsm-54103R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with ATM (Ser1981) (3F10) Monoclonal Antibody (bsm-54103R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with ATM (Ser1981) (3F10) Monoclonal Antibody (bsm-54103R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with ATM (Ser1981) (3F10) Monoclonal Antibody (bsm-54103R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54103R

Applications: WB, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Jurkat cells Probed with Phospho-Cdc6 (Ser54) (6F5) Monoclonal Antibody (bsm-52708R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Cdc6 (Ser54) (6F5) Monoclonal Antibody (bsm-52708R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Cdc6 (Ser54) (6F5) Monoclonal Antibody (bsm-52708R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52708R

Applications: WB, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Mouse Spleen; Lane 2: Mouse Lung; Lane 3: Mouse Thymus; Probed with MLKL (Ser345) (7G4) Monoclonal Antibody (bsm-54104R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Mouse Lung tissue incubated with MLKL (Ser345) (7G4) Monoclonal Antibody (bsm-54104R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse Liver tissue incubated with MLKL (Ser345) (7G4) Monoclonal Antibody (bsm-54104R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with MLKL (Ser345) (7G4) Monoclonal Antibody (bsm-54104R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54104R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

IF(ICC) staining with Phospho-alpha Synuclein (Ser129) (3G7) Monoclonal Antibody (bsm-54276R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Phospho-alpha Synuclein (Ser129) (3G7) Monoclonal Antibody (bsm-54276R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Phospho-alpha Synuclein (Ser129) (3G7) Monoclonal Antibody (bsm-54276R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with Phospho-alpha Synuclein (Ser129) (3G7) Monoclonal Antibody (bsm-54276R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54276R

Applications: WB, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: SiHa Cells; Probed with Histone H4 (acetyl K16) (2G2) Monoclonal Antibody (bsm-54330R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Histone H4 (acetyl K16) (2G2) Monoclonal Antibody (bsm-54330R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Histone H4 (acetyl K16) (2G2) Monoclonal Antibody (bsm-54330R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with Histone H4 (acetyl K16) (2G2) Monoclonal Antibody (bsm-54330R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54330R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Human Colon; Probed with CREB1 (Ser133) (5F2) Monoclonal Antibody (bsm-54334R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with CREB1 (Ser133) (5F2) Monoclonal Antibody (bsm-54334R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with CREB1 (Ser133) (5F2) Monoclonal Antibody (bsm-54334R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Spleen tissue incubated with CREB1 (Ser133) (5F2) Monoclonal Antibody (bsm-54334R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54334R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Flow cytometric analysis of Hela cells with Caspase-1 (1E8 ) Monoclonal Antibody (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Lane 1: THP-1 cells; Lane 2: Jurkat cell lysate probed with Caspase-1 (1E8 ) Monoclonal Antibody (bsm-52441R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human lung tissue incubated with Caspase-1 (1E8) Monoclonal Antibody (bsm-52441R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse liver tissue incubated with Caspase-1 (1E8) Monoclonal Antibody (bsm-52441R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52441R

Applications: WB, FCM, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of Hela cells with YB1 (2B12) Monoclonal Antibody at 1\/50 dilution (red) compared to an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Lane 1: Hela cells; Lane 2: Jurkat cells lysate probed with YB1 (2B12) Monoclonal Antibody (bsm-52444R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with YB1 (2B12) Monoclonal Antibody (bsm-52444R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with YB1 (2B12) Monoclonal Antibody (bsm-52444R) at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52444R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: NIH\/3T3 cell lysate, treated with Anisomycin; Lane 2: NIH\/3T3 cell lysate, untreated; Probed with Phospho-JNK1\/2\/3 (T183+T183+T221) (6C10) Monoclonal Antibody (bsm-52462R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Phospho-JNK1\/2\/3 (T183+T183+T221) (6C10) Monoclonal Antibody (bsm-52462R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Phospho-JNK1\/2\/3 (T183+T183+T221) (6C10) Monoclonal Antibody (bsm-52462R) at 1:100 in NIH\/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Phospho-JNK1\/2\/3 (T183+T183+T221) (6C10) Monoclonal Antibody (bsm-52462R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52462R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

A431 cell lysate probed with PRMT5 (28A3) Monoclonal Antibody (bsm-52463R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with PRMT5 (28A3) Monoclonal Antibody (bsm-52463R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with PRMT5 (28A3) Monoclonal Antibody (bsm-52463R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse kidney tissue incubated with PRMT5 (28A3) Monoclonal Antibody (bsm-52463R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52463R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Hela cells; Lane 2: HepG2 cells; Lane 3: NIH\/3T3 cells; Probed with Cyclophilin B (4A10) Monoclonal Antibody (bsm-52473R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Human Hela cell lysates; Lane 2: Human A431 cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Rat Thyroid gland lysates; Lane 5: Mouse Thyroid gland lysates; Lane 6: Rat Placenta lysates; Lane 7: Mouse Placenta lysates; Lane 8: Rat Cerebrum lysates; Lane 9: Mouse Cerebrum lysates; Lane 10: Rat Liver lysates; Lane 11: Mouse Liver lysates; Lane 12: Rat Kidney lysates; Lane 13: Mouse Kidney lysates; Lane 14: Human HUVEC cell lysates probed with Cyclophilin B (4A10) Monoclonal Antibody, Unconjugated (bsm-52473R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cyclophilin B (4A10) Monoclonal Antibody, Unconjugated (bsm-52473R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cyclophilin B (4A10) Monoclonal Antibody, Unconjugated (bsm-52473R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-52473R

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of HeLa cells with Tuberin (5B8) Monoclonal Antibody (bsm-52492R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Jurkat cell lysate probed with Tuberin (5B8) Monoclonal Antibody (bsm-52492R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Mouse placenta tissue incubated with Tuberin (5B8) Monoclonal Antibody (bsm-52492R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52492R

Applications: WB, FCM, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Hela Cells; Lane 2: MCF-7 Cells; Probed with DNA PKcs (36A1) Monoclonal Antibody (bsm-52493R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C
IF(ICC) staining with DNA PKcs (36A1) Monoclonal Antibody (bsm-52493R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52493R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of HepG2 cells with PGK1 (8C12) Monoclonal Antibody (bsm-52475R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: Hela cells; Lane 2: HepG2 cells; Lane 3: MCF-7 cells; Probed with PGK1 (8C12) Monoclonal Antibody (bsm-52475R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with PGK1 (8C12) Monoclonal Antibody (bsm-52475R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52475R

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse colon; Lane 2: MCF-7 Cells; Probed with Caspase-6 (10F5) Monoclonal Antibody (bsm-52503R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C
Paraformaldehyde-fixed and paraffin-embedded Mouse kidney tissue incubated with Caspase-6 (10F5) Monoclonal Antibody (bsm-52503R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52503R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of B16-F1 cells with MelanA (8G7) Monoclonal Antibody (bsm-52517R) at 1:50 dilution followed by secondary antibody incubation (red), compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: Melanoma Cells; Lane 2: B16-F1 Cells; Probed with MelanA (8G7) Monoclonal Antibody (bsm-52517R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-52517R

Applications: WB, FCM

Reactivity: Human, Mouse

Conjugate: Unconjugated