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Primary Antibodies

Primary Antibodies

Our Primary Antibodies are research-use antibodies designed to support protein detection and target analysis across a wide range of applications and species. Our collection includes recombinant antibodies, monoclonal antibodies, and polyclonal antibodies for use in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), flow cytometry (FC), ELISA, and other common applications.

These primary antibodies are available for targets involved in cancer research, immunology, neuroscience, cell biology, metabolism, apoptosis, signaling pathways, and disease-related research. Bioss offers primary antibodies guaranteed for use in human, mouse, rat, and other species to help researchers choose the right antibody format for their experimental model and detection method.



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Lane 1: HL-60 cell lysates probed with RCC1 Monoclonal Antibody, Unconjugated (bsm-51500M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: HL-60 cell lysates; Lane 2: Hela cell lysates; Lane 3: U-251MG cell lysates; Lane 4: MCF-7 cell lysates; Lane 5: Mouse Heart tissue lysates probed with RCC1 Monoclonal Antibody, Unconjugated (bsm-51500M) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51500m

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51504m

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51502m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51506m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51507m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: K562 cell lysates probed with RPS6KA3 Monoclonal Antibody, Unconjugated (bsm-51509M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Heart tissue lysates probed with RPS6KA3 Monoclonal Antibody, Unconjugated (bsm-51509M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51509m

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: Mouse brain tissue lysates probed with Irgm1 Monoclonal Antibody, Unconjugated (bsm-51508M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse brain tissue lysates; Lane 2: Mouse Heart tissue lysates probed with Irgm1 Monoclonal Antibody, Unconjugated (bsm-51508M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51508m

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51510m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

The TT (treated with BFA ) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Mouse Anti-CALCA antibody (bsm-51512M,1:100); Isotype Control (orange): Mouse IgG (bs-0296P). Blank control (black): PBS. Acquisition of 20,000 events was performed.\r\n
25 ug total protein per lane of various lysates (see on figure) probed with CALCA monoclonal antibody, unconjugated (bsm-51512M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-51512m-100ul

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51513m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51516m

Applications: WB, ELISA, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51514m

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PSMA5 Monoclonal Antibody, Unconjugated (bsm-51520M) at 1:25 overnight at 4\u00b0C, DAB staining.
Lane 1: K562 cell lysates probed with PSMA5 Monoclonal Antibody, Unconjugated (bsm-51520M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human liver tissue lysates; Lane 2: Hela cell lysates; Lane 3: HepG2 cell lysates; Lane 4: MCF-7 cell lysates;Lane 5: Neuro-2a cell lysates; Lane 6: PC-12 cell lysates probed with PSMA5 Monoclonal Antibody, Unconjugated (bsm-51520M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51520m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-51517m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51521m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51522m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51526m

Applications: WB

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bsm-51528m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP3A4 Monoclonal Antibody, Unconjugated (bsm-51529M) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP3A4 Monoclonal Antibody, Unconjugated (bsm-51529M) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP3A4 Monoclonal Antibody, Unconjugated (bsm-51529M) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP3A4 Monoclonal Antibody, Unconjugated (bsm-51529M) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-51529m-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51530m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51533m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51524m

Applications: WB

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bsm-51534m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51535m-100ul

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51537m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51538m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51541m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51545m

Applications: WB, IHC-P, IHC-F, IF

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-51547m

Applications: WB

Reactivity: Recombinant protein

Conjugate: Unconjugated

Catalog #1: bsm-51549m

Applications: WB

Reactivity: Recombinant protein

Conjugate: Unconjugated

Catalog #1: bsm-51550m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51554m

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51559m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Nm23\/NME1 Monoclonal Antibody, Unconjugated (bsm-51560M) at 1:25 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Nm23\/NME1 Monoclonal Antibody, Unconjugated (bsm-51560M) at 1:25 overnight at 4\u00b0C, DAB staining.
Lane 1: MCF-7 cell lysates probed with Nm23\/NME1 Monoclonal Antibody, Unconjugated (bsm-51560M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51560m

Applications: WB, IHC-P, IHC-F, IF

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-51561m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51562m

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-51564m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51567m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51569m

Applications: WB

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-51568m

Applications: WB

Reactivity: Human, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Prohibitin Monoclonal Antibody, Unconjugated (bsm-51570M) at 1:25 overnight at 4\u00b0C, DAB staining.
Lane 1: 293 cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: HepG2 cell lysates; Lane 4: NIH\/3T3 cell lysates; Lane 5: Rat Liver tissue lysates probed with Prohibitin Monoclonal Antibody, Unconjugated (bsm-51570M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51570m

Applications: WB, IHC-P, IHC-F, IF

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-51574m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51577m

Applications: WB, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GATA3 Monoclonal Antibody, Unconjugated (bsm-51575M) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: CEM cell lysates; Lane 2: HL60 cell lysates; Lane 3: SH-SY5Y cell lysates probed with GATA3 Monoclonal Antibody, Unconjugated (bsm-51575M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51575m

Applications: WB, Flow-Cyt, ICC/IF

Reactivity:

Conjugate: Unconjugated

Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with BCL10 Monoclonal Antibody(bsm-51578M)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BCL10 Monoclonal Antibody, Unconjugated(bsm-51578M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BCL10 Monoclonal Antibody, Unconjugated(bsm-51578M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BCL10 Monoclonal Antibody, Unconjugated(bsm-51578M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-51578m-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51579m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51580m

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-51582m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated