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Primary Antibodies

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Catalog #1: bsm-60737R

Applications: FCM, IHC-P, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Raw264.7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD68 Polyclonal Antibody(bs-42013R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Raw264.7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD68 Antibody(bs-42013R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-42013R

Applications: ELISA, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate: 1: HeLa (Negative control), 2: THP-1 Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 17 kDa Observed MW: 13 kDa
Tissue: Human spleen Section type: Formalin-fixed & Paraffin\r\n-embedded section Retrieval method: High temperature and high\r\npressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60643R
Tissue: Human tonsil Section type: Formalin-fixed & Paraffin\r\n-embedded section Retrieval method: High temperature and high\r\npressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60643R
Paraformaldehyde-fixed, paraffin embedded (human spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Lysozyme) Monoclonal Antibody, Unconjugated (bsm-60643R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bsm-60643R

Applications: WB, IHC-P, IHC-F

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60645R

Applications: WB

Reactivity:

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate: 1: HCT-116, 2: Raji, 3: K562, 4: C2C12, 5: PC12 Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 42 kDa Observed MW: 42 kDa
Tissue: Human bladder carcinoma Section type: Formalin-fixed &\r\nParaffin-embedded section Retrieval method: High temperature and high\r\npressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000 \r\nPrimary Ab incubation condition: 1 hour at room temperature \r\nSecondary Ab: Anti-Rabbit and Mouse\r\n Polymer HRP (Ready to use)\r\n Counter stain: Hematoxylin (Blue) \r\nComment: Color brown is the positive signal for bsm-60646R\r\n
Tissue: Human papillary thyroid carcinoma Section type: Formalin-fixed &\r\nParaffin-embedded section Retrieval method: High temperature and high\r\npressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000 \r\nPrimary Ab incubation condition: 1 hour at room temperature \r\nSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) \r\nCounter stain: Hematoxylin (Blue) \r\nComment: Color brown is the positive signal for bsm-60646R\r\n

Catalog #1: bsm-60646R

Applications: WB, IHC-P, IHC-F

Reactivity:

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: 1: HeLa, 2: A431, 3: MCF-7, 4: \r\nNIH\/3T3, 5: C6\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 23 kDa\r\nObserved MW: 23 kDa
Tissue: Human breast carcinoma\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60647R

Catalog #1: bsm-60647R

Applications: WB, IHC-P, IHC-F, ICC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60648R

Applications: WB

Reactivity:

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate: 1: PC-3, 2: Mouse brain Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 21 kDa Observed MW: 21 kDa
Tissue: Mouse colon Section type: Formalin-fixed & Paraffin\r\n-embedded section Retrieval method: High temperature and high\r\npressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60650R\r\n
Tissue: Rat colon Section type: Formalin-fixed & Paraffin\r\n-embedded section Retrieval method: High temperature and high\r\npressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60650R\r\n

Catalog #1: bsm-60650R

Applications: WB, IHC-P, IHC-F, IF, ICC

Reactivity:

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate: 1: Mouse spleen(-), 2: Mouse brain Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 15 kDa Observed MW: 13 kDa
Tissue: Human cerebrum\r\nSection type: Formalin-fixed & Paraffin\r\n-embedded section Retrieval method: High temperature and high\r\npressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60651R\r\n
Tissue: Mouse cerebrum\r\nSection type: Formalin-fixed & Paraffin\r\n-embedded section Retrieval method: High temperature and high\r\npressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60651R\r\n
Tissue: Mouse kidney (Negative tissue) Section type: Formalin-fixed & Paraffin\r\n-embedded section Retrieval method: High temperature and high\r\npressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: No staining on bsm-60651R\r\n

Catalog #1: bsm-60651R

Applications: WB, IHC-P, IHC-F

Reactivity:

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:3000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate: 1: HeLa, 2: Jurkat, 3: C6 Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 75 kDa Observed MW: 75 kDa
Tissue: Mouse liver Section type: Formalin-fixed & Paraffin\r\n-embedded section Retrieval method: High temperature and high\r\npressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60653R
Tissue: Mouse liver Section type: Formalin-fixed & Paraffin\r\n-embedded section Retrieval method: High temperature and high\r\npressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60653R\r\n

Catalog #1: bsm-60653R

Applications: WB, IHC-P, IHC-F

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60654R

Applications: WB, IHC-P, IHC-F

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60655R

Applications: WB

Reactivity:

Conjugate: Unconjugated

Tissue: Human tonsil\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60657R
Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:5000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: 1: HeLa, 2: PC-3, 3: Human liver, 4: \r\nMouse liver, 5: Rat liver\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 41 kDa\r\nObserved MW: 41 kDa
Tissue: Human liver\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60657R
Tissue: Mouse liver\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60657R

Catalog #1: bsm-60657R

Applications: WB, IHC-P, IHC-F

Reactivity:

Conjugate: Unconjugated

Tissue: Mouse colon\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:2000\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60657R
Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: 1: HeLa, 2: Jurkat, 3: HEK-293, 4: \r\nRAW 264.7, 5: C6\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 135 kDa\r\nObserved MW: 135 kDa
Tissue: Rat cerebrum\u0002Section type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60658R\r\n

Catalog #1: bsm-60658R

Applications: WB, IHC-P, IHC-F

Reactivity:

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate:(-): HeLa, (+): HeLa+ Okadaic A+ Calyculin A (100 nM, 60min) Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 84 kDa Observed MW: 84 kDa
Blocking buffer: 5% NFDM\/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate: (-): C6, (+): C6+Calyculin A(100nM, 30min) Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 84 kDa Observed MW: 84 kDa

Catalog #1: bsm-60659R

Applications: WB

Reactivity:

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate: 1: THP-1 (Negative control), 2: MOLT-4, 3: Jurkat Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 38 kDa Observed MW: 43 kDa
Tissue: Human tonsil Section type: Formalin-fixed &\r\nParaffin-embedded section Retrieval method: High temperature and high\r\npressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60660R
Tissue: Human spleen Section type: Formalin-fixed &\r\nParaffin-embedded section Retrieval method: High temperature and high\r\npressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60660R

Catalog #1: bsm-60660R

Applications: WB, IHC-P, IHC-F

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60661R

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60662R

Applications: WB

Reactivity:

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:20000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate: 1: Human heart (Negative control), 2: Human placenta Protein loading quantity: 20 \u03bcg Exposure time: 30 s Predicted MW: 35 kDa Observed MW: 35 kDa
Tissue: Human placenta Section type: Formalin-fixed &\r\nParaffin-embedded section Retrieval method: High temperature and high\r\npressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:5000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60664R
Tissue: Human prostatic hyperplasia (Negative\r\ntissue) Section type: Formalin-fixed &\r\nParaffin-embedded section Retrieval method: High temperature and high\r\npressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:5000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: No staining on bsm-60664R

Catalog #1: bsm-60664R

Applications: WB, IHC-P, IHC-F

Reactivity:

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: 1: HeLa, 2: HCT-116, 3: K562, 4: \r\nHepG2\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 29 kDa\r\nObserved MW: 35 kDa
Tissue: Human liver\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60665R
Tissue: Human spleen\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60665R
Tissue: Mouse liver\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60665R

Catalog #1: bsm-60665R

Applications: WB, IHC-P, IHC-F

Reactivity:

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: 1. Jurkat (Negative control), 2: Ramos, \r\n3: Raji, 4: Daudi, 5: Mouse spleen, 6: Rat \r\nspleen\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 42 kDa\r\nObserved MW: 45 kDa
Tissue: Human appendix\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60667R
Tissue: Human kidney (Negative tissue)\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: No staining on nucleus.

Catalog #1: bsm-60667R

Applications: WB, IHC-P, IHC-F

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60669R

Applications: WB

Reactivity:

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: 1: MCF7, 2: HeLa, 3: A549\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 56 kDa\r\nObserved MW: 56 kDa
Tissue: Human cerebrum\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60670R
Tissue: Human colon carcinoma\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60670R
Tissue: Mouse cerebrum\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60670R

Catalog #1: bsm-60670R

Applications: WB, IHC-P, IHC-F

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60671R

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60672R

Applications: WB

Reactivity:

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate: 1: Jurkat, 2: Raji, 3: NIH\/3T3, 4: C6 Protein loading quantity: 20 \u03bcg Exposure time: 30 s Predicted MW: 97 kDa Observed MW: 120 kDa
Tissue: Human tonsil Section type: Formalin-fixed & Paraffin\r\n-embedded section Retrieval method: High temperature and high\r\npressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60673R\r\n
Tissue: Mouse colon Section type: Formalin-fixed & Paraffin\r\n-embedded section Retrieval method: High temperature and high\r\npressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60673R\r\n
Tissue: Rat cardiac muscle Section type: Formalin-fixed & Paraffin\r\n-embedded section Retrieval method: High temperature and high\r\npressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60673R\r\n

Catalog #1: bsm-60673R

Applications: WB, IHC-P, IHC-F, ICC

Reactivity:

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate: 1: Human placenta, 2: Human liver Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 34 kDa Observed MW: 34 kDa
Tissue: Human placenta Section type: Formalin-fixed &\r\nParaffin-embedded section Retrieval method: High temperature and high\r\npressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal forbsm-60674R\r\n
Tissue: Human liver Section type: Formalin-fixed &\r\nParaffin-embedded section Retrieval method: High temperature and high\r\npressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60674R\r\n

Catalog #1: bsm-60674R

Applications: WB, IHC-P, IHC-F

Reactivity:

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate: 1: HeLa, 2: HepG2, 3: PC-12 Protein loading quantity: 20 \u03bcg Exposure time: 30 s Predicted MW: 24 kDa Observed MW: 24 kDa
Tissue: Human colon Section type: Formalin-fixed & Paraffin\r\n-embedded section Retrieval method: High temperature and high\r\npressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60675R\r\n
Tissue: Mouse testis Section type: Formalin-fixed & Paraffin\r\n-embedded section Retrieval method: High temperature and high\r\npressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60675R\r\n
Tissue: Rat cerebrum\r\nSection type: Formalin-fixed & Paraffin\r\n-embedded section Retrieval method: High temperature and high\r\npressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60675R\r\n

Catalog #1: bsm-60675R

Applications: WB, IHC-P, IHC-F, IF, ICC

Reactivity:

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:5000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: 1: MCF7, 2: A549, 3: Jurkat, 4: U87-\r\nMG, 5: Mouse brain, 6: Rat brain\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 85 kDa\r\nObserved MW: 85 kDa
Tissue: Human placenta\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nforbsm-60676R\r\n
Tissue: Mouse brain\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor PTM-6610
Tissue: Rat brain\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60676R\r\n

Catalog #1: bsm-60676R

Applications: WB, IHC-P, IHC-F, IF, ICC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60688R

Applications: WB, IHC-P, IHC-F

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60678R

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60679R

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60680R

Applications: WB, IF, ICC

Reactivity:

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: HepG2\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 61 kDa\r\nObserved MW: 61 kDa
Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:5000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: C6\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 61 kDa\r\nObserved MW: 61 kDa
Tissue: Human breast carcinoma\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60681R
Tissue: Human cerebrum\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60681R

Catalog #1: bsm-60681R

Applications: WB, IHC-P, IHC-F

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60682R

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60683R

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60684R

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60685R

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60686R

Applications: WB, IF, ICC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60687R

Applications: WB, IF, ICC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60652R

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60690R

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60691R

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60692R

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60693R

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60694R

Applications: WB

Reactivity:

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:5000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: 1: Daudi, 2: Ramos\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 68 kDa\r\nObserved MW: 68 kDa
Tissue: Human tonsil\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60695R\r\n
Tissue: Human testis\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60695R\r\n

Catalog #1: bsm-60695R

Applications: WB, IHC-P, IHC-F

Reactivity:

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: 1: HepG2, 2: HeLa\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 94 kDa\r\nObserved MW: 100 kDa
Tissue: Human thyroid cancer\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60696R

Catalog #1: bsm-60696R

Applications: WB, IHC-P, IHC-F, IF, ICC

Reactivity:

Conjugate: Unconjugated