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Primary Antibodies

Primary Antibodies

Our Primary Antibodies are research-use antibodies designed to support protein detection and target analysis across a wide range of applications and species. Our collection includes recombinant antibodies, monoclonal antibodies, and polyclonal antibodies for use in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), flow cytometry (FC), ELISA, and other common applications.

These primary antibodies are available for targets involved in cancer research, immunology, neuroscience, cell biology, metabolism, apoptosis, signaling pathways, and disease-related research. Bioss offers primary antibodies guaranteed for use in human, mouse, rat, and other species to help researchers choose the right antibody format for their experimental model and detection method.



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25 ug total protein per lane of various lysates (see on figure) probed with HLA-DRA monoclonal antibody, unconjugated (bsm-51091M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with HLA-DRA Monoclonal Antibody, Unconjugated(bsm-51091M) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with HLA-DRA Monoclonal Antibody, Unconjugated(bsm-51091M) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Esophagus Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with HLA-DRA Monoclonal Antibody, Unconjugated(bsm-51091M) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-51091m-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: Unconjugated

Lane 1: CEM lysates; Lane 2: MDA-MB-453 lysates; probed with MAPK3 (7C2) Monoclonal Antibody, unconjugated (bsm-51092M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Mouse heart lysates probed with MAPK3 (7C2) Monoclonal Antibody, unconjugated (bsm-51092M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-51092M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51093M

Applications: WB, IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51096M

Applications: WB, IF(ICC), IF

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-51098M

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51099M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: A431; Lane 2: HeLa cell lysate at 20 \u00b5g per lane. probed with bsm-51102M EIF4B (355CT12.6.5) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Jurkat cell lysate probed with bsm-51102M EIF4B (355CT12.6.5) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.

Catalog #1: bsm-51102M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded human lymph tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with Kappa light chain (HP6053) Monoclonal Antibody (bsm-51106M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: Human Serum 8\u03bcl Lysates. Probed with Human Ig Kappa light chain monoclonal Antibody, unconjugated (bsm-51106M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51106M

Applications: WB, ELISA, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51103M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51105M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: A431; Lane 2: HeLa lysate at 20 \u00b5g per lane, probed with bsm-51104M ACTA1 (337CT30.10.1) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Paraformaldehyde-fixed, paraffin embedded human colon carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ACTA1 (337CT30.10.1) Monoclonal Antibody (bsm-51104M) at 1:50 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: K562; Lane 2: CEM lysate at 20 \u00b5g per lane, probed with bsm-51104M ACTA1 (337CT30.10.1) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.

Catalog #1: bsm-51104M

Applications: WB, FCM, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51113M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51111M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51108M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51112M

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51119M

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51116M

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51117M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51114M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51118M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51120M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51121M

Applications: WB

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bsm-51122M

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51124M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51128M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

SH-SY5Y (20\u00b5g) probed with bsm-51134M GABARAP (424CT5.1.6) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Mouse brain lysate (20\u00b5g) probed with bsm-51134M GABARAP (424CT5.1.6) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.

Catalog #1: bsm-51134M

Applications: WB, ELISA

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51135M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Human brain (20\u00b5g) probed with bsm-51143M GAD2 (458CT17.2.4) Monoclonal Antibody at 1:500 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Lane 1: rat brain, Lane 2: mouse brain lysate probed with bsm-51143M GAD2 (458CT17.2.4) Monoclonal Antibody at 1:500 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.

Catalog #1: bsm-51143M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-51141M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

NCCIT cell lysate probed with bsm-51142M SOX2 (7C4) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Lane 1: SOX2 Protein Isolate; probed with SOX2 (7C4) Monoclonal Antibody, unconjugated (bsm-51142M) at 1:4000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with SOX2 (7C4) Monoclonal Antibody (bsm-51142M) at 1:50 (37\u2103 for 1hr) in A549 cells (green). Cells were counterstained with Alexa Fluor\u00ae 555 (red) conjugated Phalloidin. Cells were fixed in 4% paraformaldehyde for 20min and permeabilized with 0.1% Triton X100\/PBS for 10min.
Paraformaldehyde-fixed and paraffin-embedded Human Lung Cancer tissue incubated with SOX2 (7C4) Monoclonal Antibody (bsm-51142M) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-51142M

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51147m-100ul

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51151M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51153M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51159M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51158M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51168M

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-51160M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51161M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51162m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51163M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51165M

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-51167M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

HeLa cell lysates; probed with STRA8 (526CT11.1.5) Monoclonal Antibody, unconjugated (bsm-51172M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Mouse bladder lysates; probed with STRA8 (526CT11.1.5) Monoclonal Antibody, unconjugated (bsm-51172M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-51172M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51171M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51173M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51176M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51180M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51175M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated