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Primary Antibodies

Primary Antibodies

Our Primary Antibodies are research-use antibodies designed to support protein detection and target analysis across a wide range of applications and species. Our collection includes recombinant antibodies, monoclonal antibodies, and polyclonal antibodies for use in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), flow cytometry (FC), ELISA, and other common applications.

These primary antibodies are available for targets involved in cancer research, immunology, neuroscience, cell biology, metabolism, apoptosis, signaling pathways, and disease-related research. Bioss offers primary antibodies guaranteed for use in human, mouse, rat, and other species to help researchers choose the right antibody format for their experimental model and detection method.



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Catalog #1: bsm-51017M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51021M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: K562 Cell lysates; probed with LTF (119CT80.1.1) Monoclonal Antibody, unconjugated (bsm-51024M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells, stained with LTF (119CT80.1.1) Monoclonal Antibody (bsm-51024M) at 1:25 dilution, followed by Goat anti-mouse IgG secondary antibody at 1:200 dilution. Immunofluorescence image shows cytoplasm staining in HeLa cells (green), actin (red), and nuclear counter stain with DAPI (blue).

Catalog #1: bsm-51024M

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51034M

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: MCF-7 cell lysate; Lane 2: K562 cell lysate at 20 \u00b5g per lane probed with with bsm-51025M BRAF (125CT13.3.5) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Lane 1: HL-60 Cell lysates; probed with BRAF (125CT13.3.5) Monoclonal Antibody, unconjugated (bsm-51025M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), and incubated with BRAF (125CT13.3.5) Monoclonal Antibody (bsm-51025M) at 1:25 dilution for 1 hr at 37\u2103. Followed by Alexa Fluor\u00ae 488 conjugated donkey anti-mouse antibody at 1:400 dilution for 50 min at 37\u2103. Image shows cytoplasm BRAF staining (green), and actin (red).

Catalog #1: bsm-51025M

Applications: WB, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51026M

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51027M

Applications: WB, FCM, IHC-P, IF

Reactivity: Human

Conjugate: Unconjugated

HT-1080 Cells lysates probed with MMP14 (3A6) Monoclonal Antibody, unconjugated (bsm-51028M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at Room Temperature.
Lane 1: A2058 Cell lysates; probed with MMP14 (3A6) Monoclonal Antibody, unconjugated (bsm-51028M) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HepG2 cells; Antibody incubation with MMP14 (3A6) Monoclonal Antibody, Unconjugated (bsm-51028M) at 1:25 overnight at 4\u00b0C, followed by a conjugated secondary DAPI and for nuclei staining.

Catalog #1: bsm-51028M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51029M

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51031M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

K562 cell lysate (20ug) probed with bsm-51033M ACOX1 (153CT43.1.1) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Paraformaldehyde-fixed, paraffin embedded human colon tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ACOX1 (153CT43.1.1) Monoclonal Antibody (bsm-51033M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51033M

Applications: WB, IHC-P, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51037M

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51046M

Applications: WB, IHC-P, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

293T\/17 cell lysate (20ug) probed with bsm-51038M MCFD2 (165CT13.1.6) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
U251MG cell lysate (20ug) probed with bsm-51038M MCFD2 (165CT13.1.6) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
K562 cell lysate (20ug) probed with bsm-51038M MCFD2 (165CT13.1.6) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.

Catalog #1: bsm-51038M

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51040M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51041M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51042M

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PDK2 Monoclonal Antibody, Unconjugated (bsm-51043M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PDK2 Monoclonal Antibody, Unconjugated (bsm-51043M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PDK2 Monoclonal Antibody, Unconjugated (bsm-51043M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with PDK2 monoclonal antibody, unconjugated (bsm-51043M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-51043m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51044M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51051M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: 293; Lane 2: K562 whole cell lysate at 20 \u00b5g per lane, probed with bsm-51058M TGFB2 (220ct16.4.3.1) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Paraformaldehyde-fixed, paraffin embedded human colon carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with TGFB2 (220ct16.4.3.1) Monoclonal Antibody (bsm-51058M) at 1:50 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human breast carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with TGFB2 (220ct16.4.3.1) Monoclonal Antibody (bsm-51058M) at 1:50 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51058M

Applications: WB, IHC-P, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51052M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: 293; Lane 2: Hela whole cell lysate at 20 \u00b5g per lane; probed with bsm-51055M PRKAA2 (206CT5.4.2) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), incubated with PRKAA2 (206CT5.4.2) Monoclonal Antibody (bsm-51055M) at 1:25 dilution for 1 h at 37\u2103, followed by secondary antibody incubation (green), actin (red), and nuclear counter stain with DAPI (blue).

Catalog #1: bsm-51055M

Applications: WB, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51057M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51054M

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-51059M

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51060M

Applications: WB, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51062M

Applications: WB

Reactivity: Human, Other Species

Conjugate: Unconjugated

Catalog #1: bsm-51061M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51064M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human pancreatic cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD44 (H3) Monoclonal Antibody, Unconjugated (bsm-51065M) at 1:200 overnight at 4\u00b0C, DAB staining.
Hela cells were\u00a0 incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with CD44 (H3) Monoclonal Antibody(bsm-51065M)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).
25 ug total protein per lane of various lysates (see on figure) probed with CD44 monoclonal antibody, unconjugated (bsm-51065M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD44 Monoclonal Antibody, Unconjugated (bsm-51065M) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-51065m

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: HepG2 Cell lysates; Lane 2: MCF-7 Cell lysates; probed with MAP4K3 (219CT8.3.1) Monoclonal Antibody, unconjugated (bsm-51067M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human skeletal muscle tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with MAP4K3 (219CT8.3.1) Monoclonal Antibody (bsm-51067M) at 1:100 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51067M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Lane 1: HeLa; Lane 2: HCT116 lysate at 20 \u00b5g per lane. probed with bsm-51077M RAB8A (261CT1.3.1) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Lane 1: Mouse brain lysates; probed with RAB8A (261CT1.3.1) Monoclonal Antibody, unconjugated (bsm-51077M) at 1:100 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: U937 cell lysates probed with RAB8A (8A4) Monoclonal Antibody, Unconjugated (bsm-51077M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates probed with RAB8A (8A4) Monoclonal Antibody, Unconjugated (bsm-51077M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51077M

Applications: WB, ELISA

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51068M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51070M

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51071M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

SH-SY5Y lysates (20ug) probed with INA (257CT7.1.2) Monoclonal Antibody, unconjugated (bsm-51074M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Mouse brain cell lysates; probed with INA (257CT7.1.2) Monoclonal Antibody, unconjugated (bsm-51074M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-51074M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51075M

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51076M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51079M

Applications: WB, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51082M

Applications: WB, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51083M

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51086M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51084M

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51087M

Applications: WB, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51089M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51095M

Applications: WB, FCM

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51090M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated