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Primary Antibodies

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Paraformaldehyde-fixed, paraffin embedded human hepatocellular carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Serum albumin (3F4) Monoclonal Antibody, Unconjugated (bsm-0945M) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
A549 lysates probed with Serum albumin Polyclonal Antibody, Unconjugated (bsm-0945M) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Lung lysates; Lane 2: Mouse Spleen lysates probed with Serum albumin Polyclonal Antibody, Unconjugated (bsm-0945M) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1:Human Serum probed with Serum albumin Monoclonal Antibody, Unconjugated (bsm-0945M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #: bsm-0945M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #: bsm-0945M-A350

Applications: IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: ALEXA FLUOR® 350

Catalog #: bsm-0945M-A594

Applications: IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: ALEXA FLUOR® 594

Catalog #: bsm-0945M-A488

Applications: IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: ALEXA FLUOR® 488

Catalog #: bsm-0945M-A555

Applications: IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: ALEXA FLUOR® 555

Catalog #: bsm-0945M-Cy3

Applications: IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #: bsm-0945M-A647

Applications: IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: ALEXA FLUOR® 647

Catalog #: bsm-0945M-Cy5

Applications: IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Cy5

Catalog #: bsm-0945M-Cy7

Applications: IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Cy7

Catalog #: bsm-0945M-FITC

Applications: IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: FITC

Catalog #: bsm-0945M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #: bsm-0945M-A680

Applications: IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: ALEXA FLUOR® 680

Catalog #: bsm-0945M-A750

Applications: IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: ALEXA FLUOR® 750

Catalog #: bsm-0945M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #: bsm-50100M

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-50101M

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-50105M

Applications: WB, IF(ICC), IP

Reactivity: Human

Conjugate: Unconjugated

Lane 1 : Prx1 Recombinant Protein; Lane 2 : Prx2 Recombinant Protein; Lane 3 : Prx3 Recombinant Protein; Lane 4 : Prx4 Recombinant Protein; Lane 5 : Prx5 Recombinant Protein; Lane 6 : Prx6 Recombinant Protein; probed with Peroxiredoxin VI (1A11) Monoclonal Antibody, unconjugated (bsm-50107M) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:5000 for 60 minutes at Room Temperature.
Immunoprecipitation of HeLa cell lysates using Peroxiredoxin VI (1A11) Monoclonal Antibody (bsm-50107M): Lane 1 : HeLa Cell Lysate; Lane 2 : Precipitated from 200ug of Cell Lysate using 2ug of antibody; Lane 3 : Precipitated from 200ug of Cell Lysate using 5ug of antibody; Lane 4 : Precipitated from PBS using 5ug of antibody. WB analysis was performed using bsm-50172M at 0.5ug\/mL (1:2000) and incubated at 4\u2103 overnight, followed secondary antibody incubation for 60min at Room Temperature.\r\n

Catalog #: bsm-50107M

Applications: WB, IF(ICC), IP

Reactivity: Human

Conjugate: Unconjugated

Lane 1 : TR1 Recombinant Protein; Lane 2 : TR2 Recombinant Protein, probed with Thioredoxin Reductase 1 (19A1) Monoclonal Antibody, unconjugated (bsm-50109M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:5000 for 60 minutes at Room Temperature.
Immunoprecipitation of HeLa cell lysates using Thioredoxin Reductase 1 (19A1) Monoclonal Antibody (bsm-50109M): Lane 1 : HeLa Cell Lysate; Lane 2 : Precipitated from 200ug of Cell Lysate using 2ug of antibody; Lane 3 : Precipitated from 200ug of Cell Lysate using 5ug of antibody; Lane 4 : Precipitated from PBS using 5ug of antibody. WB analysis was performed using bsm-50109M at 1.0ug\/mL (1:1000) and incubated at 4\u2103 overnight, followed secondary antibody incubation for 60min at Room Temperature.\r\n

Catalog #: bsm-50109M

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-50114M

Applications: IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-50117M

Applications: IP

Reactivity: Human

Conjugate: Unconjugated

Lane 1 : HeLa Cell Lysate; Lane 2 : 293T Cell Lysate probed with Peroxiredoxin III (4G10) Monoclonal Antibody, unconjugated (bsm-50128M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:5000 for 60 minutes at Room Temperature.
Lane 1 : Prx1 Recombinant Protein; Lane 2 : Prx2 Recombinant Protein; Lane 3 : Prx3 Recombinant Protein; Lane 4 : Prx4 Recombinant Protein; Lane 5 : Prx5 Recombinant Protein; Lane 6 : Prx6 Recombinant Protein; probed with Peroxiredoxin III (4G10) Monoclonal Antibody, unconjugated (bsm-50128M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:5000 for 60 minutes at Room Temperature.
Immunoprecipitation of HeLa cell lysates using Peroxiredoxin III (4G10) Monoclonal Antibody (bsm-50128M): Lane 1 : HeLa Cell Lysate; Lane 2 : Precipitated from 200ug of Cell Lysate using 2ug of antibody; Lane 3 : Precipitated from 200ug of Cell Lysate using 5ug of antibody; Lane 4 : Precipitated from PBS using 5ug of antibody. WB analysis was performed using bsm-50128M at 0.5ug\/mL (1:2000) and incubated at 4\u2103 overnight, followed secondary antibody incubation for 60min at Room Temperature.

Catalog #: bsm-50128M

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Lane 1 : TR1 Recombinant Protein; Lane 2 : TR2 Recombinant Protein, probed with Thioredoxin Reductase 2 (25B3)\u00a0Monoclonal Antibody, unconjugated (bsm-50136M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:5000 for 60 minutes at Room Temperature.
Immunoprecipitation of HeLa cell lysates using Thioredoxin Reductase 2 (25B3) Monoclonal Antibody (bsm-50136M): Lane 1 : HeLa Cell Lysate; Lane 2 : Precipitated from 200ug of Cell Lysate using 2ug of antibody; Lane 3 : Precipitated from 200ug of Cell Lysate using 5ug of antibody; Lane 4 : Precipitated from PBS using 5ug of antibody. WB analysis was performed using bsm-50552M at 0.5ug\/mL (1:2000) and incubated at 4\u2103 overnight, followed secondary antibody incubation for 60min at Room Temperature.

Catalog #: bsm-50136M

Applications: IP

Reactivity: Human

Conjugate: Unconjugated

Immunoprecipitation of HeLa cell lysates using Peroxiredoxin \u2165 (5E1) Monoclonal Antibody (bsm-50143M):\r\n\r\nLane 1 : HeLa Cell Lysate;\r\nLane 2 : Precipitated from 200ug of Cell Lysate using 2ug of antibody;\r\nLane 3 : Precipitated from 200ug of Cell Lysate using 5ug of antibody;\r\nLane 4 : Precipitated from PBS using 5ug of antibody.\r\n\r\nWB analysis was performed using bsm-50172M at 1.0ug\/mL (1:1000)\r\nand incubated at 4\u2103 overnight, followed secondary antibody incubation\r\nfor 60min at Room Temperature.
HeLa cells were stained with Peroxiredoxin \u2165 (5E1) Monoclonal Antibody (bsm-50143M) at [1:200] incubated for 2Hr at RT, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

Catalog #: bsm-50143M

Applications: IHC-P, IF(ICC), IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-50154M

Applications: IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-50156M

Applications: IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-50164M

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Lane 1 : U87mg Cell Lysate probed with Transglutaminase 2 (14G2) Monoclonal Antibody, unconjugated (bsm-50165M) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:5000 for 60 minutes at Room Temperature.
Immunoprecipitation of U-87 MG cell lysates using Transglutaminase 2 (14G2) Monoclonal Antibody (bsm-50165M): Lane 1 : U-87 MG Cell Lysate; Lane 2 : Precipitated from 200ug of Cell Lysate using 2ug of antibody; Lane 3 : Precipitated from 200ug of Cell Lysate using 5ug of antibody; Lane 4 : Precipitated from PBS using 5ug of antibody. WB analysis was performed using bsm-50164M at 0.5ug\/mL (1:2000) and incubated at 4\u2103 overnight, followed secondary antibody incubation for 60min at Room Temperature.

Catalog #: bsm-50165M

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Lane 1 : HeLa Cell Lysate; Lane 2 : 293T Cell Lysate probed with Peroxiredoxin \u2165 (6H5) Monoclonal Antibody, unconjugated (bsm-50172M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:5000 for 60 minutes at room temperature.
Immunoprecipitation of HeLa cell lysates using Peroxiredoxin \u2165 (6H5) Monoclonal Antibody (bsm-50172M): Lane 1 : HeLa Cell Lysate; Lane 2 : Precipitated from 200ug of Cell Lysate using 2ug of antibody; Lane 3 : Precipitated from 200ug of Cell Lysate using 5ug of antibody; Lane 4 : Precipitated from PBS using 5ug of antibody. WB analysis was performed using bsm-50172M at 0.5ug\/mL (1:2000) and incubated at 4\u2103 overnight, followed secondary antibody incubation for 60min at 37˚C.
Western-blot Analysis: Lane 1 : Prx1 Recombinant Protein; Lane 2 : Prx2 Recombinant Protein; Lane 3 : Prx3 Recombinant Protein; Lane 4 : Prx4 Recombinant Protein; Lane 5 : Prx5 Recombinant Protein; Lane 6 : Prx6 Recombinant Protein, probed with Peroxiredoxin \u2165 (6H5) Monoclonal Antibody, unconjugated (bsm-50172M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:5000 for 60 minutes at room temperature.

Catalog #: bsm-50172M

Applications: WB, IF(ICC), IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-50194M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-50196M

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-50198M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Cross-reactivity experiment. Lane 1 : Prx1 Recombinant Protein; Lane 2 : Prx2 Recombinant Protein; Lane 3 : Prx3 Recombinant Protein; Lane 4 : Prx4 Recombinant Protein; Lane 5 : Prx5 Recombinant Protein; Lane 6 : Prx6 Recombinant Protein; probed with Peroxiredoxin II (1E8) Monoclonal Antibody (bsm-50201M) at 0.5ug\/mL (1:2000) and incubated at 4\u2103 overnight, followed secondary antibody incubation for 60min at room temperature.
Lane 1 : HeLa Cell Lysate; Lane 2 : 293T Cell Lysate; Lane 3 : Mouse Brain Tissue; Lane 4 : Rat Brain Tissue; probed with Peroxiredoxin II (1E8) Monoclonal Antibody (bsm-50201M) at 0.5ug\/mL (1:2000) and incubated at 4\u2103 overnight, followed secondary antibody incubation for 60min at room temperature.

Catalog #: bsm-50201M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1 : HeLa Cell Lysate; Lane 2 : Jurkat Cell Lysate; Lane 3 : 293T Cell Lysate; Lane 4 : K562 Cell Lysate; Lane 5 : L929 Cell Lysate probed with bsm-50218M IKK-\u03b1 (3A11) Monoclonal Antibody at 2.0ug\/mL (1:500) dilution and incubated at 4\u2103 overnight, followed by secondary antibody incubation for 60min at room temperature.
Lane 1 : I\u03baB kinase alpha Recombinant Protein; Lane 2 : I\u03baB kinase beta Recombinant Protein; Lane 3 : I\u03baB kinase gamma Recombinant Protein probed with bsm-50218M IKK-\u03b1 (3A11) Monoclonal Antibody at 1.0ug\/mL (1:1000) dilution and incubated at 4\u2103 overnight, followed by secondary antibody incubation for 60min at room temperature.
Immunoprecipitation of IKK-\u03b1 from 293T cells using IKK-\u03b1 (3A11) Monoclonal Antibody (bsm-50218M). Lane 1 : 293T Cell Lysate; Lane 2 : Precipitated from 293T Cell Lysate (200ug) using 2ug of antibody; Lane 3 : Precipitated from 293T Cell Lysate (200ug) using 5ug of antibody; Lane 4 : Precipitated from PBS using 5ug of antibody. WB was performed using at 2.0ug\/mL (1:500) dilution and incubated at 4\u2103 overnight, followed by secondary antibody incubation for 60min at room temperature.

Catalog #: bsm-50218M

Applications: WB, IP

Reactivity: Human, Mouse

Conjugate: Unconjugated

Human Serum (1.0uL) probed with bsm-50217M Serum amyloid P component (6E6) Monoclonal Antibody at 2.0ug\/mL (1:500) dilution and incubated at 4\u2103 overnight, followed secondary antibody incubation for 60min at room temperature.
Immunoprecipitation of Serum amyloid P component from human serum using Serum amyloid P component (6E6) Monoclonal Antibody (bsm-50217M). Lane 1 : Human Serum (1.0uL); Lane 2 : Precipitated from 1ul of Human Serum using 2ug of antibody; Lane 3 : Precipitated from 1ul of Human Serum using 5ug of antibody; Lane 4 : Precipitated from PBS using 5ug of antibody. WB analysis was performed using bsm-50217M at 2.0ug\/mL (1:500) and incubated at 4\u2103 overnight, followed by secondary antibody incubation for 60min at room temperature.

Catalog #: bsm-50217M

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-50223M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-50232M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-50267M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-50281M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-50289M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

HepG2 Cell lysates probed with AFP (\u03b1-fetoprotein) (3B1)\u00a0 Monoclonal Antibody, unconjugated (bsm-50334M) at 1:250 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:5000 for 60 minutes at Room Temperature.
Immunoprecipitation of AFP (\u03b1-fetoprotein) from HepG2 cell lysates using AFP (\u03b1-fetoprotein) (3B1)\u00a0 Monoclonal Antibody (bsm-50334M): Lane 1 : HepG2 Cell Lysate; Lane 2 : Precipitated from 200ug of Cell Lysate using 2ug of antibody; Lane 3 : Precipitated from 200ug of Cell Lysate using 5ug of antibody; Lane 4 : Precipitated from PBS using 5ug of antibody. WB analysis was performed using bsm-50334M at 4.0ug\/mL (1:250) and incubated at 4\u2103 overnight, followed secondary antibody incubation for 60min at Room Temperature.

Catalog #: bsm-50334M

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-50402M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-50403M

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Lane 1: HepG2 Cell lysates; Lane 2: K562 Cell lysates; Lane 3: NCI-H460 Cell lysates; probed with ALDH1A1 (2C2) Monoclonal Antibody, unconjugated (bsm-51032M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: NCI-H460 Cells; 15 ug of protein lysate loaded; Probed with ALDH1A1 (2C2) Monoclonal Antibody (bsm-51032M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with ALDH1A1 (2C2) Monoclonal Antibody (bsm-51032M) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
IF(ICC) staining with ALDH1A1 (2C2) Monoclonal Antibody (bsm-51032M) at 1:25 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cytoplasmic actin is detected with Dylight\u00ae 554 Phalloidin (red). Cells were fixed in 4% paraformaldehyde, permeabilized with 0.1% Triton X100\/PBS.

Catalog #: bsm-51032M

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Hela cells probed with HSPA1A (201CT8.2.3) Monoclonal Antibody (bsm-51050M) at 1:50 dilution, followed by secondary antibody incubation (green), and DAPI nuclear staining (blue).
Lane 1: HL-60 Cells; Probed with HSPA1A (1C3) Monoclonal Antibody, Unconjugated (bsm-51050M) at 1:1000, overnight at 4\u00b0C followed by incubation with a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #: bsm-51050M

Applications: WB, IHC-P, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-51056M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: 293 Cell lysates; Lane 2: CEM Cell lysates; Lane 3: HepG2 Cell lysates; Lane 4: Jurkat Cell lysates; Lane 5: HeLa Cell lysates; Lane 6: Raji Cell lysates; probed with H2AFX (8C5) Monoclonal Antibody, unconjugated (bsm-51222M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human Thymus tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with H2AFX (8C5) Monoclonal Antibody (bsm-51222M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human prostate tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with H2AFX (8C5) Monoclonal Antibody (bsm-51222M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #: bsm-51222M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-51225M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated