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Catalog #: bsm-50147M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1 : Human Serum (0.5uL); Lane 2 : Human Serum (1.0uL); probed with bsm-50242M A1BG (54B12) Monoclonal Antibody at 0.5ug/mL (1:2000) and incubated at 4℃ overnight, followed by secondary antibody incubation for 60min at room temperature.
Immunoprecipitation of A1BG from human serum using A1BG (54B12) Monoclonal Antibody (bsm-50242M). Lane 1: Human Serum (0.5uL); Lane 2: Precipitated from Human Serum (1uL) using 2ug of antibody; Lane 3: Precipitated from Human Serum (1uL) using 5ug of antibody; Lane 4: Precipitated from PBS using 5ug of antibody. WB analysis was performed using bsm-50242M at 0.5ug/mL (1:2000) and incubated at 4℃ overnight, followed by secondary antibody incubation for 60min at room temperature.

Catalog #: bsm-50242M

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-51296M

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #: bsm-50337M

Applications: WB

Reactivity: Human, Rat

Conjugate: Unconjugated

K562 cell lysate (20ug) probed with bsm-51033M ACOX1 (153CT43.1.1) Monoclonal Antibody at 1:1000 dilution and 4℃ overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Paraformaldehyde-fixed, paraffin embedded human colon tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ACOX1 (153CT43.1.1) Monoclonal Antibody (bsm-51033M) at 1:25 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #: bsm-51033M

Applications: WB, IHC-P, IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: A431; Lane 2: HeLa lysate at 20 µg per lane, probed with bsm-51104M ACTA1 (337CT30.10.1) Monoclonal Antibody at 1:1000 dilution and 4℃ overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Paraformaldehyde-fixed, paraffin embedded human colon carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ACTA1 (337CT30.10.1) Monoclonal Antibody (bsm-51104M) at 1:50 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: K562; Lane 2: CEM lysate at 20 µg per lane, probed with bsm-51104M ACTA1 (337CT30.10.1) Monoclonal Antibody at 1:1000 dilution and 4℃ overnight incubation, followed by secondary antibody incubation for 60min at room temperature.

Catalog #: bsm-51104M

Applications: WB, FCM, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-51447M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

HepG2 Cell lysates probed with AFP (α-fetoprotein) (3B1)  Monoclonal Antibody, unconjugated (bsm-50334M) at 1:250 overnight at 4°C followed by a conjugated secondary antibody at 1:5000 for 60 minutes at Room Temperature.
Immunoprecipitation of AFP (α-fetoprotein) from HepG2 cell lysates using AFP (α-fetoprotein) (3B1)  Monoclonal Antibody (bsm-50334M): Lane 1 : HepG2 Cell Lysate; Lane 2 : Precipitated from 200ug of Cell Lysate using 2ug of antibody; Lane 3 : Precipitated from 200ug of Cell Lysate using 5ug of antibody; Lane 4 : Precipitated from PBS using 5ug of antibody. WB analysis was performed using bsm-50334M at 4.0ug/mL (1:250) and incubated at 4℃ overnight, followed secondary antibody incubation for 60min at Room Temperature.

Catalog #: bsm-50334M

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-50299M

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-51238M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: MCF-7; Lane 2: Jurkat; Lane 3: PC-12 cell lysate (20µg per lane) probed with bsm-51346M AKT2 (1623CT791.157.67.66) Monoclonal Antibody at 1:1000 dilution and 4℃ overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa cells labeled with AKT2 (16C6) Monoclonal Antibody at 1:25 dilution, followed by goat anti-mouse IgG secondary antibody (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red). Nuclear counter stain is DAPI (blue).

Catalog #: bsm-51346M

Applications: WB, IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Lane 1: HepG2 Cell lysates; Lane 2: K562 Cell lysates; Lane 3: NCI-H460 Cell lysates; probed with ALDH1A1 (2C2) Monoclonal Antibody, unconjugated (bsm-51032M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: NCI-H460 Cells; 15 ug of protein lysate loaded; Probed with ALDH1A1 (2C2) Monoclonal Antibody (bsm-51032M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with ALDH1A1 (2C2) Monoclonal Antibody (bsm-51032M) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
IF(ICC) staining with ALDH1A1 (2C2) Monoclonal Antibody (bsm-51032M) at 1:25 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cytoplasmic actin is detected with Dylight® 554 Phalloidin (red). Cells were fixed in 4% paraformaldehyde, permeabilized with 0.1% Triton X100/PBS.

Catalog #: bsm-51032M

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Lane 1: NCI-H292 Cell lysates; Lane 2: A549 Cell lysates; Lane 3: HepG2 Cell lysates; probed with ALDH2 (138CT22.3.8) Monoclonal Antibody, unconjugated (bsm-51417M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed, paraffin embedded Human brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ANTIBODY Monoclonal Antibody (bsm-51417M) at 1:50 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #: bsm-51417M

Applications: WB, FCM, IHC-P, IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: HepG2 Cell lysates; Lane 2: T47D Cell lysates; probed with ALDH6A1 (147CT8.3.4) Monoclonal Antibody, unconjugated (bsm-51430M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed, paraffin embedded human stomach tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ALDH6A1 (147CT8.3.4) Monoclonal Antibody (bsm-51430M) at 1:25 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human liver tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ALDH6A1 (147CT8.3.4) Monoclonal Antibody (bsm-51430M) at 1:25 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #: bsm-51430M

Applications: WB, IHC-P, IF

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse liver tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with alpha Tubulin (4G1) monoclonal Antibody, Unconjugated (bsm-50179M) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
RSC96 probed with alpha Tubulin (4G1) Monoclonal Antibody, Unconjugated (bsm-50179M) at 1:100 for 30 minutes followed by incubation with a FITC conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Human cervical carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with alpha Tubulin (4G1) monoclonal Antibody, Unconjugated (bsm-50179M) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
Lane 1: Hela; Lane 2: 293T; Lane 3: MDA-MB-231; Lane 4: A549; Lane 5: A431; Lane 6: RSC96; Lane 7: VSC4.1; Lane 8: ASPC-1; Lane 9: B16; Lane 10: BV2; Lane 11: H9C2; Lane 12: Hep2G;Lane 13: K562; Lane 14: U937; probed with alpha Tubulin (4G1) Monoclonal Antibody, unconjugated (bsm-50179M) at 1:2000 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.\r\n

Catalog #: bsm-50179M

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

RSC96 probed with alpha Tubulin (4G1) Monoclonal Antibody, Unconjugated (bsm-50180M) at 1:100 for 30 minutes followed by incubation with a FITC conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Mouse liver tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with alpha Tubulin (4G1) monoclonal Antibody, Unconjugated (bsm-50180M) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded Human cervical carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with alpha Tubulin (4G1) monoclonal Antibody, Unconjugated (bsm-50180M) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
Lane 1: Hela; Lane 2: 293T; Lane 3: MDA-MB-231; Lane 4: A549; Lane 5: A431; Lane 6: RSC96; Lane 7: VSC4.1; Lane 8: ASPC-1; Lane 9: B16; Lane 10: BV2; Lane 11: H9C2; Lane 12: Hep2G;Lane 13: K562; Lane 14: U937; probed with alpha Tubulin (4G1) Monoclonal Antibody, unconjugated (bsm-50180M) at 1:2000 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.

Catalog #: bsm-50180M

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-50280M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-50281M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-50141M

Applications: WB, IHC-P, IP

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-50130M

Applications: WB, IHC-P, IP

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-50174M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: HeLa; Lane 2: K562 Cell lysates probed with ANXA1 (4C1) Monoclonal Antibody, unconjugated (bsm-51219M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed, paraffin embedded human esophagus tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ANXA1 (4C1) Monoclonal Antibody (bsm-51219M) at 1:25 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #: bsm-51219M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Lane 1: A431; Lane 2: HeLa; Lane 3: HepG2; Lane 4: K562; Lane 5: PC-3 cell lysate at 20 µg per lane, probed with bsm-51448M APEX1 (1518CT337.123.86.269.232) Monoclonal Antibody at 1:2000 dilution and 4℃ overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Paraformaldehyde-fixed, paraffin embedded human stomach tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with APEX1 (15C2) Monoclonal Antibody (bsm-51448M) at 1:25 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #: bsm-51448M

Applications: WB

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #: bsm-50186M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Lane 1: THP-1 Cell lysates; Lane 2: mouse liver lysates; Lane 3: mouse testis lysates; probed with ATG3 (7C2) Monoclonal Antibody, unconjugated (bsm-51284M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed, paraffin embedded Human skeletal muscle tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ATG3 (7C2) Monoclonal Antibody (bsm-51284M) at 1:25 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ATG3 (7C2) Monoclonal Antibody (bsm-51284M) at 1:25 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human skeletal muscle tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ATG3 (7C2) Monoclonal Antibody (bsm-51285M) at 1:25 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #: bsm-51284M

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #: bsm-51084M

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #: bsm-51061M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-51277M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-50203M

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Lane 1: HT-29 Cell lysates; probed with AURKA (4C5) Monoclonal Antibody, unconjugated (bsm-51279M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed, paraffin embedded human brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with AURKA (4C5) Monoclonal Antibody (bsm-51279M) at 1:25 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #: bsm-51279M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-51349M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded human brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with BAP31 (2C6) Monoclonal Antibody (bsm-51442M) at 1:25 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: A431 Cell lysates; Lane 2: NCCIT Cell lysates; probed with BAP31 (2C6) Monoclonal Antibody, unconjugated (bsm-51442M) at 1:2000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

Catalog #: bsm-51442M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-51256M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1 : MCF7 Cell Lysate; Lane 2 : HL60 Cell Lysate; Lane 3 : SK-N-MC Cell Lysate; probed with Bcl-2 (2B11) Monoclonal Antibody (bsm-50256M) at 2.0ug/mL and incubated at 4℃ overnight, followed by secondary antibody incubation for 60min at room temperature.
Immunoprecipitation of Bcl-2 from HL60 cells using Bcl-2 (2B11) Monoclonal Antibody (bsm-50256M). Lane 1 : HL60 Cell Lysate; Lane 2 : Precipitated from HL60 Cell Lysate (200ug) using 2ug of antibody; Lane 3 : Precipitated from HL60 Cell Lysate (200ug) using 5ug of antibody; Lane 4 : Precipitated from PBS using 5ug of antibody; WB analysis was performed using bsm-50256M at 2.0ug/mL and incubated at 4℃ overnight, followed by secondary antibody incubation for 60min at room temperature.

Catalog #: bsm-50256M

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Daudi Cell lysates; Lane 2: Raji Cell lysates; probed with BCL10 (5C2) Monoclonal Antibody, unconjugated (bsm-51398M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed, paraffin embedded Human Kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with BCL10 (5C2) Monoclonal Antibody (bsm-51398M) at 1:25 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Hela cells probed with using BCL10 (5C2) Monoclonal Antibody (green) unconjugated bsm-51398M at 1:25 for 60 minutes at 37ºC followed by secondary antibody at 1:400 compared to an isotype control of mouse IgG (blue).
Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Blocked with 3% BSA for 30min at RT; Antigen retrieval via heat mediation with a citrate buffer (pH6); Antibody incubation with BCL10 (5C2) Monoclonal Antibody, Unconjugated (bsm-51398M) at 1:25 for 1hr at 37°C, followed by a conjugated secondary antibody and DAB staining.

Catalog #: bsm-51398M

Applications: WB, FCM, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-51229M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-51096M

Applications: WB, IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Jurkat Cell lysates; Lane 2: A431 Cell lysates; Lane 3: THP-1 Cell lysates; Lane 4: 293T/17 Cell lysates; probed with BID (9C7) Monoclonal Antibody, unconjugated (bsm-51306M) at 1:x000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 cells labeled with BID (9C7) Monoclonal Antibody (bsm-51306M) at 1/25 dilution, followed by secondary antibody (green), cytoplasmic actin staining (red), and nuclear counter stain with DAPI (blue).
Paraformaldehyde-fixed, paraffin embedded human bladder tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with BID (9C7) Monoclonal Antibody (bsm-51306M) at 1:25 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #: bsm-51306M

Applications: WB, IHC-P, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-51082M

Applications: WB, IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: MCF-7 cell lysate; Lane 2: K562 cell lysate at 20 µg per lane probed with with bsm-51025M BRAF (125CT13.3.5) Monoclonal Antibody at 1:1000 dilution and 4℃ overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Lane 1: HL-60 Cell lysates; probed with BRAF (125CT13.3.5) Monoclonal Antibody, unconjugated (bsm-51025M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), and incubated with BRAF (125CT13.3.5) Monoclonal Antibody (bsm-51025M) at 1:25 dilution for 1 hr at 37℃. Followed by Alexa Fluor® 488 conjugated donkey anti-mouse antibody at 1:400 dilution for 50 min at 37℃. Image shows cytoplasm BRAF staining (green), and actin (red).

Catalog #: bsm-51025M

Applications: WB, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-50248M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-51151M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-51057M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: A431 Cell lysates; Lane 2: T47D Cell lysates; probed with CDH1 (9C8) Monoclonal Antibody, unconjugated (bsm-51351M) at 1:4000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed, paraffin embedded human colon tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CDH1 (9C8) Monoclonal Antibody (bsm-51351M) at 1:25 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized MCF-7 cells; antibody incubation with CDH1 (9C8) Monoclonal Antibody, Unconjugated (bsm-51351M) at 1:25 overnight at 4°C, followed by a conjugated secondary DAPI and for nuclei staining.

Catalog #: bsm-51351M

Applications: WB, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Lane 1: MCF-7 Cell lysates; Lane 2: A549 Cell lysates; Lane 3: A431 Cell lysates; probed with CAPN1 (6C5) Monoclonal Antibody, unconjugated (bsm-51419M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CAPN1 (6C5) Monoclonal Antibody (bsm-51419M) at 1:25 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Hela cells probed with using CAPN1 (6C5) Monoclonal Antibody bsm-51419M at 1:25 for 60 minutes followed by A488 secondary antibody at 1:400 (green), compared to an isotype control of mouse IgG1 (blue).

Catalog #: bsm-51419M

Applications: WB, FCM, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Human Placenta lysates; probed with CAPN6 (5C5) Monoclonal Antibody, unconjugated (bsm-51290M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
A549 cells probed with using CAPN6 (5C5) Monoclonal Antibody bsm-51290M at 1:25 for 60 minutes at 37ºC followed by secondary antibody at 1:400 (green), compared to an isotype control of mouse IgG1 (blue).

Catalog #: bsm-51290M

Applications: WB, FCM

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #: bsm-50260M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-51119M

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated