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Primary Antibodies

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Paraformaldehyde-fixed, paraffin embedded human hepatocellular carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Serum albumin (3F4) Monoclonal Antibody, Unconjugated (bsm-0945M) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
A549 lysates probed with Serum albumin Polyclonal Antibody, Unconjugated (bsm-0945M) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Lung lysates; Lane 2: Mouse Spleen lysates probed with Serum albumin Polyclonal Antibody, Unconjugated (bsm-0945M) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1:Human Serum probed with Serum albumin Monoclonal Antibody, Unconjugated (bsm-0945M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #: bsm-0945M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #: bsm-0945M-A350

Applications: IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: ALEXA FLUOR® 350

Catalog #: bsm-0945M-A594

Applications: IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: ALEXA FLUOR® 594

Catalog #: bsm-0945M-A488

Applications: IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: ALEXA FLUOR® 488

Catalog #: bsm-0945M-A555

Applications: IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: ALEXA FLUOR® 555

Catalog #: bsm-0945M-Cy3

Applications: IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #: bsm-0945M-A647

Applications: IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: ALEXA FLUOR® 647

Catalog #: bsm-0945M-Cy5

Applications: IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Cy5

Catalog #: bsm-0945M-Cy7

Applications: IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Cy7

Catalog #: bsm-0945M-FITC

Applications: IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: FITC

Catalog #: bsm-0945M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #: bsm-0945M-A680

Applications: IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: ALEXA FLUOR® 680

Catalog #: bsm-0945M-A750

Applications: IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: ALEXA FLUOR® 750

Catalog #: bsm-0945M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Biotin

Lane 1: HepG2 Cell lysates; Lane 2: K562 Cell lysates; Lane 3: NCI-H460 Cell lysates; probed with ALDH1A1 (2C2) Monoclonal Antibody, unconjugated (bsm-51032M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: NCI-H460 Cells; 15 ug of protein lysate loaded; Probed with ALDH1A1 (2C2) Monoclonal Antibody (bsm-51032M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with ALDH1A1 (2C2) Monoclonal Antibody (bsm-51032M) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
IF(ICC) staining with ALDH1A1 (2C2) Monoclonal Antibody (bsm-51032M) at 1:25 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cytoplasmic actin is detected with Dylight\u00ae 554 Phalloidin (red). Cells were fixed in 4% paraformaldehyde, permeabilized with 0.1% Triton X100\/PBS.

Catalog #: bsm-51032M

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Hela cells probed with HSPA1A (201CT8.2.3) Monoclonal Antibody (bsm-51050M) at 1:50 dilution, followed by secondary antibody incubation (green), and DAPI nuclear staining (blue).
Lane 1: HL-60 Cells; Probed with HSPA1A (1C3) Monoclonal Antibody, Unconjugated (bsm-51050M) at 1:1000, overnight at 4\u00b0C followed by incubation with a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #: bsm-51050M

Applications: WB, IHC-P, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-51056M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: 293 Cell lysates; Lane 2: CEM Cell lysates; Lane 3: HepG2 Cell lysates; Lane 4: Jurkat Cell lysates; Lane 5: HeLa Cell lysates; Lane 6: Raji Cell lysates; probed with H2AFX (8C5) Monoclonal Antibody, unconjugated (bsm-51222M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human Thymus tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with H2AFX (8C5) Monoclonal Antibody (bsm-51222M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human prostate tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with H2AFX (8C5) Monoclonal Antibody (bsm-51222M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with H2AFX (8C5) Monoclonal Antibody, Unconjugated (bsm-51222M) at 1:50 overnight at 4\u00b0C, DAB staining.

Catalog #: bsm-51222M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-51225M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-51238M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-51300M

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Lane 1: HL-60; Lane 2: K562 cell lysate at 20 \u00b5g per lane, probed with bsm-51424M ITGB2 (534CT6.2.1) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Paraformaldehyde-fixed, paraffin embedded human tonsil tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ITGB2 (534CT6.2.1) Monoclonal Antibody (bsm-51424M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #: bsm-51424M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-52001M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Western blot analysis of 4E-BP1 expression in non-transfected (A) and mouse 4E-BP1 transfected (B) 293T whole cell lysates.
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of glandular cells using 4E-BP1 (3G1) Monoclonal Antibody (bsm-52007R)

Catalog #: bsm-52007R

Applications: WB, IHC-P, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-52037M

Applications: WB, IHC-P, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: 293; Lane 2: F9; Lane 3: PC12; Lane 4: Hela lysates probed with Cyclin A2 (2E9) Monoclonal Antibody (bsm-52043M) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Paraformaldehyde-fixed, paraffin embedded human uterus \r\ntissue; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Cyclin A2 (2E9) Monoclonal Antibody (bsm-52043M) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #: bsm-52043M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-52067R

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-1240M

Applications: IHC-P, IF(IHC-P)

Reactivity: Human, Others

Conjugate: Unconjugated

Catalog #: bsm-1240M-Cy5

Applications: IF(IHC-P)

Reactivity: Human, Others

Conjugate: Cy5

Catalog #: bsm-1240M-Biotin

Applications: IHC-P

Reactivity: Human, Others

Conjugate: Biotin

Catalog #: bsm-1240M-HRP

Applications: IHC-P

Reactivity: Human, Others

Conjugate: HRP

Catalog #: bsm-1240M-A350

Applications: IF(IHC-P)

Reactivity: Human, Others

Conjugate: ALEXA FLUOR® 350

Catalog #: bsm-1240M-A488

Applications: IF(IHC-P)

Reactivity: Human, Others

Conjugate: ALEXA FLUOR® 488

Catalog #: bsm-1240M-A594

Applications: IF(IHC-P)

Reactivity: Human, Others

Conjugate: ALEXA FLUOR® 594

Catalog #: bsm-1240M-A647

Applications: IF(IHC-P)

Reactivity: Human, Others

Conjugate: ALEXA FLUOR® 647

Catalog #: bsm-1240M-A555

Applications: IF(IHC-P)

Reactivity: Human, Others

Conjugate: ALEXA FLUOR® 555

Catalog #: bsm-1240M-Cy3

Applications: IF(IHC-P)

Reactivity: Human, Others

Conjugate: Cy3

Catalog #: bsm-1240M-Cy7

Applications: IF(IHC-P)

Reactivity: Human, Others

Conjugate: Cy7

Catalog #: bsm-1240M-FITC

Applications: IF(IHC-P)

Reactivity: Human, Others

Conjugate: FITC

Catalog #: bsm-1240M-A680

Applications: IF(IHC-P)

Reactivity: Human, Others

Conjugate: ALEXA FLUOR® 680

Catalog #: bsm-1240M-A750

Applications: IF(IHC-P)

Reactivity: Human, Others

Conjugate: ALEXA FLUOR® 750

Lane 1 : Bosc23 cell lysate transfected with HA\/HSP60; Lane 2 : Bosc23 cell lysate transfected with Myc\/His\/HePTP; Lane 3 : Bosc23 cell lysate transfected with GFP\/Ub+1 lysates, probed with HA tag (7H5) Monoclonal Antibody, unconjugated (bsm-50131M) at 1:10000 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:5000 for 60 minutes at Room Temperature.
Immunoprecipitation of Bosc23 cell lysates using HA tag (7H5) \r\nMonoclonal Antibody (bsm-50131M): Lane 1 : Bosc23 cell lysate transfected with HA\/HSP60; Lane 2: Precipitated from 200ug Bosc23 transfected with HA\/HSP60 using 5ug of antibody; Lane 3 : Precipitated from PBS using 5ug of antibody; WB analysis was performed using bsm-50131M at 0.1ug\/mL (1:10000) and incubated at 4\u2103 overnight, followed secondary antibody incubation for 60min at Room Temperature.

Catalog #: bsm-50131M

Applications: WB, IF(ICC), IP

Reactivity: Human, All

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse liver tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with alpha Tubulin (4G1) monoclonal Antibody, Unconjugated (bsm-50179M) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
RSC96 probed with alpha Tubulin (4G1) Monoclonal Antibody, Unconjugated (bsm-50179M) at 1:100 for 30 minutes followed by incubation with a FITC conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Human cervical carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with alpha Tubulin (4G1) monoclonal Antibody, Unconjugated (bsm-50179M) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Lane 1: Hela; Lane 2: 293T; Lane 3: MDA-MB-231; Lane 4: A549; Lane 5: A431; Lane 6: RSC96; Lane 7: VSC4.1; Lane 8: ASPC-1; Lane 9: B16; Lane 10: BV2; Lane 11: H9C2; Lane 12: Hep2G;Lane 13: K562; Lane 14: U937; probed with alpha Tubulin (4G1) Monoclonal Antibody, unconjugated (bsm-50179M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.\r\n

Catalog #: bsm-50179M

Applications: WB, FCM, IHC-P

Reactivity: Human, Mouse, Rat, All

Conjugate: Unconjugated

Catalog #: bsm-50185M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1 : A431 Cell Lysate; Lane 2 : HeLa Cell Lysate; probed PLK1 (18D4) Monoclonal Antibody \uff08bsm-50251M) at 2.0ug\/mL (1:500) and incubated at 4\u2103 overnight, followed by secondary antibody incubation for 60min at room temperature.
Immunoprecipitation of PLK1 from A431 cells using PLK1 (18D4) Monoclonal Antibody (bsm-50251M). Lane 1 : A431 Cell Lysate; Lane 2 : Precipitated from A431 Cell Lysate (200ug) using 1ug of antibody; Lane 3 : Precipitated from A431 Cell Lysate (200ug) using 2ug of antibody; Lane 4 : Precipitated from PBS using 2ug of antibody. WB analysis was performed using bsm-50251M at 2.0ug\/mL (1:500) and incubated at 4\u2103 overnight, followed by secondary antibody incubation for 60min at room temperature.

Catalog #: bsm-50251M

Applications: WB, ELISA, IP, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-50258M

Applications: WB, ELISA, IHC, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-50330M

Applications: WB, ELISA, IHC-P, IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1 : MCF7 Cell Lysate; Lane 2 : A431 Cell Lysate; Lane 3 : C6 Cell Lysate; Lane 4 : L929 Cell Lysate; probed with using bsm-50375M at Cyclin D1 (2G5) Monoclonal Antibody 4.0ug\/mL (1:250) and incubated at 4\u2103 overnight, followed by secondary antibody incubation for 60min at room temperature.
Lane 1 : Cyclin B1 Recombinant Protein; Lane 2 : Cyclin D1 Recombinant Protein; Lane 3 : Cyclin D2 Recombinant Protein; Lane 4 : Cyclin D3 Recombinant Protein; Lane 5 : Cyclin E1 Recombinant Protein; Lane 6 : Cyclin E2 Recombinant Protein; probed with using bsm-50375M at Cyclin D1 (2G5) Monoclonal Antibody 0.5ug\/mL (1:2000) and incubated at 4\u2103 overnight, followed by secondary antibody incubation for 60min at room temperature.

Catalog #: bsm-50375M

Applications: WB, IP

Reactivity: Human, Rat

Conjugate: Unconjugated