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Primary Antibodies

Primary Antibodies

Our Primary Antibodies are research-use antibodies designed to support protein detection and target analysis across a wide range of applications and species. Our collection includes recombinant antibodies, monoclonal antibodies, and polyclonal antibodies for use in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), flow cytometry (FC), ELISA, and other common applications.

These primary antibodies are available for targets involved in cancer research, immunology, neuroscience, cell biology, metabolism, apoptosis, signaling pathways, and disease-related research. Bioss offers primary antibodies guaranteed for use in human, mouse, rat, and other species to help researchers choose the right antibody format for their experimental model and detection method.



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Lane 1: HeLa Cell lysates; Lane 2: MCF-7 Cell lysates; Lane 3: K562 Cell lysates; Lane 4: NIH\/3T3 Cell lysates; Lane 5: PC-12 Cell lysates; probed with IF4E (4C5) Monoclonal Antibody, unconjugated (bsm-51305M) at 1:4000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 cells labeled with IF4E (4C5) Monoclonal Antibody (bsm-51305M) at 1\/25 dilution, followed by secondary antibody (green), cytoplasmic actin staining (red), and nuclear counter stain with DAPI (blue).

Catalog #1: bsm-51305M

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (LAMP1) monoclonal Antibody, unconjugated (bsm-51301M) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-LAMP1 antibody (bsm-51301M): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488 (bs-60296G-BF488): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
The HT-1080 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-LAMP1 antibody (bsm-51030M): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488 (bs-60296G-BF488): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
25 ug total protein per lane of various lysates (see on figure) probed with LAMP1 monoclonal antibody, unconjugated (bsm-51301M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-51301m

Applications: WB, IHC-P, IF

Reactivity: Human

Conjugate: Unconjugated

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS cells labeled with HINT1 (5C3) Monoclonal Antibody (bsm-51304M) at 1\/25 dilution, followed by secondary antibody (green), cytoplasmic actin staining (red), and nuclear counter stain with DAPI (blue).
Paraformaldehyde-fixed, paraffin embedded Human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with HINT1 (5C3) Monoclonal Antibody (bsm-51304M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Jurkat cells probed with using HINT1 (5C3) Monoclonal Antibody bsm-51304M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:400 compared (green) compared to an isotype control of mouse IgG1 (blue).
Lane 1: Jurkat; Lane 2: Raji; Lane 3: HepG2; Lane 4: Rat Brain lysates; Lane 5: Mouse Liver lysates; probed with HINT1 (5C3) Polyclonal Antibody, unconjugated (bsm-51304M) at 1:4000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-51304M

Applications: WB, FCM, IHC-P, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded human skin tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CSF1R (6C7) Monoclonal Antibody (bsm-51303M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining
Human placenta lysates probed with CSF1R (6C7) Monoclonal Antibody, unconjugated (bsm-51303M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human skin tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CSF1R (6C7) Monoclonal Antibody (bsm-51303M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
U-87MG cells probed with using CSF1R (6C7) Monoclonal Antibody bsm-51303M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:400 (green) compared to an isotype control of mouse IgG1 (blue).

Catalog #1: bsm-51303M

Applications: WB, ELISA, FCM, IHC-P

Reactivity:

Conjugate: Unconjugated

Lane 1: THP-1 Cell lysates; Lane 2: HL-60 Cell lysates; probed with HCK (8C1) Monoclonal Antibody, unconjugated (bsm-51307M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 cells labeled with HCK (8C1) Monoclonal Antibody (bsm-51307M) at 1\/25 dilution, followed by secondary antibody (green), cytoplasmic actin staining (red), and nuclear counter stain with DAPI (blue).

Catalog #1: bsm-51307M

Applications: WB, IF(ICC), IF

Reactivity:

Conjugate: Unconjugated

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeled with IRF3 (2C4) Monoclonal Antibody (bsm-51312M) at 1\/25 dilution, followed by secondary antibody (green), cytoplasmic actin staining (red), and nuclear counter stain with DAPI (blue).
Jurkat cells probed with using IRF3 (2C4) Monoclonal Antibody bsm-51312M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:400 (green) compared to an isotype control of mouse IgG (blue).
Lane 1: COS-7; Lane 2: Daudi; Lane 3: HepG2; Lane 4: HT-29; Lane 5: Jurkat; Lane 6: MCF-7; probed with IRF3 (2C4) Polyclonal Antibody, unconjugated (bsm-51312M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Blocked with 3% BSA for 30min at RT; Antibody incubation with IRF3 (2C4) Monoclonal Antibody, Unconjugated (bsm-51312M) at 1:25 for 1hr at 37\u00b0C, followed by a conjugated secondary antibody and DAB staining.

Catalog #1: bsm-51312M

Applications: WB, FCM, IHC-P, IF(ICC), IF

Reactivity:

Conjugate: Unconjugated

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 cells labeled with KRT19 (979CT14.3.1) Monoclonal Antibody (bsm-51308M) at 1\/25 dilution, followed by secondary antibody (green) and nuclear counter stain with DAPI (blue).
Paraformaldehyde-fixed, paraffin embedded Human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with KRT19 (979CT14.3.1) Monoclonal Antibody (bsm-51308M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51308M

Applications: WB, IHC-P, IF(ICC), IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: mouse brain lysates; Lane 2: rat brain lysates; probed with RAB3A (5B7) Monoclonal Antibody, unconjugated (bsm-51311M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PC-12 cells labeled with RAB3A (5B7) Monoclonal Antibody (bsm-51311M) at 1\/25 dilution, followed by secondary antibody (green), cytoplasmic actin staining (red), and nuclear counter stain with DAPI (blue).
human brain lysates probed with RAB3A (5B7) Polyclonal Antibody, unconjugated (bsm-51311M) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
PC-12 cells probed with using RAB3A (5B7) Monoclonal Antibody, bsm-51311M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:400 (green) compared to an isotype control of mouse IgG (blue).

Catalog #1: bsm-51311M

Applications: WB, FCM, IF(ICC), IF

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51309M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Jurkat Cell lysates; Lane 2: A431 Cell lysates; Lane 3: THP-1 Cell lysates; Lane 4: 293T\/17 Cell lysates; probed with BID (9C7) Monoclonal Antibody, unconjugated (bsm-51306M) at 1:x000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 cells labeled with BID (9C7) Monoclonal Antibody (bsm-51306M) at 1\/25 dilution, followed by secondary antibody (green), cytoplasmic actin staining (red), and nuclear counter stain with DAPI (blue).
Paraformaldehyde-fixed, paraffin embedded human bladder tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with BID (9C7) Monoclonal Antibody (bsm-51306M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51306M

Applications: WB, IHC-P, IF(ICC), IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51317M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat cells labeled with LCK (6C6) Monoclonal Antibody (bsm-51313M) at 1\/25 dilution, followed by secondary antibody (green), cytoplasmic actin staining (red), and nuclear counter stain with DAPI (blue).
Jurkat cell lysates probed with LCK (6C6) Polyclonal Antibody, unconjugated (bsm-51313M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Jurkat cells probed with using LCK (6C6) Monoclonal Antibody bsm-51313M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody (green) at 1:400 compared to an isotype control of mouse IgG (blue).

Catalog #1: bsm-51313M

Applications: WB, FCM, IHC-P, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Lane 1: mouse brain lysates; Lane 2: rat brain lysates; Lane 3: SW480 Cell lysates; Lane 4: human brain lysates; probed with RAB3B (7F5) Monoclonal Antibody, unconjugated (bsm-51316M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human pancreas tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with RAB3B (7F5) Monoclonal Antibody (bsm-51316M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
HepG2 cells probed with using RAB3B (7F5) Monoclonal Antibody (green) unconjugated bsm-51316M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:400 compared to an isotype control of mouse IgG (blue).
Lane 1: Cerebrum lysates probed with RAB3B (7F5) Monoclonal Antibody, Unconjugated (bsm-51316M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51316M

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded human brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with SODM (9C3) Monoclonal Antibody (bsm-51319M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: human brain lysate; Lane 2: Hela cell lysate; Lane 3: MCF-7 cell lysate; Lane 4: NIH\/3T3 cell lysate; Lane 5: SH-SY5Y cell lysate; probed with SODM (9C3) Polyclonal Antibody, unconjugated (bsm-51319M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-51319M

Applications: WB, FCM, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: SW480 Cell lysates; probed with TIMP2 (4C7) Monoclonal Antibody, unconjugated (bsm-51320M) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 cells labeled with TIMP2 (4C7) Monoclonal Antibody (bsm-51320M) at 1\/25 dilution followed by secondary antibody (green), cytoplasmic actin staining (red), and nuclear counter stain with DAPI (blue).
K562 cells probed with using TIMP2 (4C7) Monoclonal Antibody bsm-51320M at 1:25 for 30 minutes followed by A488 secondary antibody (green) at 1:200, compared to an isotype control of mouse IgG1 (blue).
Paraformaldehyde-fixed, paraffin embedded Human kidney tissue; Blocked with 3% BSA for 30min at RT; Antibody incubation with TIMP2 (4C7) Monoclonal Antibody, Unconjugated (bsm-51320M ) at 1:25 for 1hr at 37\u00b0C, followed by a conjugated secondary antibody and DAB staining.

Catalog #1: bsm-51320M

Applications: WB, FCM, IHC-P, IF(ICC), IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: HeLa; Lane 2: Jurkat; Lane 3: C2C12; Lane 4: PC-12; Lane 5: rat brain; Lane 6: 293T\/17 lysates (20\u00b5g) probed with bsm-51322M RAB11A (6C8) Monoclonal Antibody at 1:4000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Paraformaldehyde-fixed, paraffin embedded human brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with RAB11A (6C8) Monoclonal Antibody (bsm-51322M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51322M

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-51321M

Applications: WB

Reactivity: All

Conjugate: Unconjugated

Catalog #1: bsm-51326M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

A431 cell lysates probed with NMI (8C9) Polyclonal Antibody, unconjugated (bsm-51329M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
K562 cells probed with using NMI (8C9) Monoclonal Antibody (green) unconjugated bsm-51329M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:400 compared to an isotype control of mouse IgG1 (blue).
Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells; Antibody incubation with NMI (8C9) Monoclonal Antibody, Unconjugated (bsm-51329M) at 1:25 overnight at 4\u00b0C, followed by a conjugated secondary (green). Cytoplasmic actin is detected with Dylight\u00ae 554 Phalloidin at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).

Catalog #1: bsm-51329M

Applications: WB, FCM, IF(ICC), IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51327M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51328M

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51337M

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells; Antibody incubation with S100A2 (2C1) Monoclonal Antibody, Unconjugated (bsm-51330M) at 1:25 overnight at 4\u00b0C, followed by a conjugated secondary (green). Cytoplasmic actin is detected with Dylight\u00ae 554 Phalloidin at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with S100A2 Monoclonal Antibody, Unconjugated (bsm-51330M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with S100A2 Monoclonal Antibody, Unconjugated (bsm-51330M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with S100A2 Monoclonal Antibody, Unconjugated (bsm-51330M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-51330m

Applications: IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: DU145 Cell lysates; Lane 2: mouse stomach Cell lysates; probed with RAB27B (4G7) Monoclonal Antibody, unconjugated (bsm-51331M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
A431 cells probed with using RAB27B (4G7) Monoclonal Antibody bsm-51331M at 1:25 for 60 minutes followed by A488 secondary antibody at 1:200 (green) compared to an isotype control of mouse IgG1 (blue).
Lane 1: A549 cell lysates probed with RAB27B (4G7) Monoclonal Antibody, Unconjugated (bsm-51331M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Bladder lysates probed with RAB27B (4G7) Monoclonal Antibody, Unconjugated (bsm-51331M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51331M

Applications: WB, FCM

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51332M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: PC-3 Cell lysates; Lane 2: mouse brain lysates; Lane 3: rat brain lysates; Lane 4: human brain lysates; Lane 5: human testis lysates; probed with RAB18 (3A8) Monoclonal Antibody, unconjugated (bsm-51333M) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
PC-3 cells probed with using RAB18 (3A8) Monoclonal Antibody bsm-51333M at 1:25 for 60 minutes followed by A488 secondary antibody at 1:200 (green), compared to an isotype control of mouse IgG1 (blue).

Catalog #1: bsm-51333M

Applications: WB, FCM, IF(IHC-P)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-51335M

Applications: WB

Reactivity:

Conjugate: Unconjugated

Lane 1: K562 Cell lysates; probed with RAB27A (9C6) Monoclonal Antibody, unconjugated (bsm-51334M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
K562 cells probed with using RAB27A (9C6) Monoclonal Antibody bsm-51334M at 1:25 for 60 minutes followed by A488 secondary antibody at 1:200 (green) compared to an isotype control of mouse IgG1 (blue).
Lane 1: Human K562 cell lysates; Lane 2: Human Jurkat cell lysates probed with RAB27A Monoclonal Antibody, Unconjugated (bsm-51334M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51334M

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with RFA2 (5C3) Monoclonal Antibody (bsm-51336M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: Hela; Lane 2: MCF-7; Lane 3: T47D; Lane 4: Ramos; Lane 5: 293T\/17 cell lysate probed with RFA2 (5C3) Polyclonal Antibody, unconjugated (bsm-51336M) at 1:4000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-51336M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51340M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51338M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

A431 cell lysate (20ug) probed with RAB5B (5C5) Monoclonal Antibody, unconjugated (bsm-51341M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at Room Temperature.
Lane 1: HeLa Cell lysates; Lane 2: HepG2 Cell lysates; Lane 3: Jurkat Cell lysates; Lane 4: NIH-3T3 Cell lysates; probed with RAB5B (5C5) Monoclonal Antibody, unconjugated (bsm-51341M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-51341M

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-51345M

Applications: WB, IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: MCF-7; Lane 2: Jurkat; Lane 3: PC-12 cell lysate (20\u00b5g per lane) probed with bsm-51346M AKT2 (1623CT791.157.67.66) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa cells labeled with AKT2 (16C6) Monoclonal Antibody at 1:25 dilution, followed by goat anti-mouse IgG secondary antibody (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight\u00ae 554 Phalloidin at 1\/100 dilution (red). Nuclear counter stain is DAPI (blue).

Catalog #1: bsm-51346M

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized MCF-7 cells; antibody incubation with CDH1 (9C8) Monoclonal Antibody, Unconjugated (bsm-51351M) at 1:25 overnight at 4\u00b0C, followed by a conjugated secondary DAPI and for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with E cadherin Monoclonal Antibody, Unconjugated (bsm-51351M) at 1:1000 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with E cadherin Monoclonal Antibody, Unconjugated (bsm-51351M) at 1:1000 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with E cadherin Monoclonal Antibody, Unconjugated (bsm-51351M) at 1:1000 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-51351m-100ul

Applications: IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-51349M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Jurkat Cell lysates; Lane 2: Ramos Cell lysates; Lane 3: HT-1080 Cell lysates; probed with GARS (1C6) Monoclonal Antibody, unconjugated (bsm-51350M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells; antibody incubation with GARS (1C6) Monoclonal Antibody, Unconjugated (bsm-51350M) at 1:25 overnight at 4\u00b0C, followed by a conjugated secondary (green). Cytoplasmic actin is detected with Dylight\u00ae 554 Phalloidin at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).
Hela cells probed with using GARS (1C6) Monoclonal Antibody (green) unconjugated bsm-51350M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:200 compared to an isotype control of mouse IgG1 (blue).

Catalog #1: bsm-51350M

Applications: WB, FCM, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51359M

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51353M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51354M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51356M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: MCF-7; Lane 2: A431; Lane 3: A549; Lane 4: U-87MG cell lysate at 20 \u00b5g per lane, probed with bsm-51357M RAB13 (5C9) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Lane 1: MCF-7 Cell lysates; Lane 2: A549 Cell lysates; Lane 3: A431 Cell lysates; probed with RAB13 (5C9) Monoclonal Antibody, unconjugated (bsm-51357M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-51357M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: HeLa Cell lysates; Lane 2: A431 Cell lysates; Lane 3: U-20S Cell lysates; probed with RAB1B (3C3) Monoclonal Antibody, unconjugated (bsm-51363M) at 1:4000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
A431 cells probed with using RAB1B (3C3) Monoclonal Antibody (green) unconjugated bsm-51363M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:200 compared to an isotype control of mouse IgG1 (blue).

Catalog #1: bsm-51363M

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51360M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51362M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51373m-100ul

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: HeLa Cell lysates; Lane 2: A431 Cell lysates; Lane 3: PC-12 Cell lysates; Lane 4: COS-7 Cell lysates; Lane 5: NIH\/3T3 Cell lysates; probed with MP2K1 (8C8) Monoclonal Antibody, unconjugated (bsm-51364M) at 1:4000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Hela cells probed with using MP2K1 (8C8) Monoclonal Antibody (green) unconjugated bsm-51364M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:200 compared to an isotype control of mouse IgG1 (blue).

Catalog #1: bsm-51364M

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Human lung lysates; probed with RYK (1C1) Monoclonal Antibody, unconjugated (bsm-51365M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
A431 cells probed with RYK (1C1) Monoclonal Antibody (green) unconjugated bsm-51365M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:200 (green), compared to an isotype control of mouse IgG1 (blue).

Catalog #1: bsm-51365M

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated