Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch

Primary Antibodies

Collection Menu

Catalog #: bsm-50357M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Immunoprecipitation of HeLa cell lysates using Bcl-2 (11C5) Monoclonal Antibody (bsm-50257M): Lane 1 : HeLa Cell Lysate; Lane 2 : Precipitated from 200ug of Cell Lysate using 2ug of antibody; Lane 3 : Precipitated from 200ug of Cell Lysate using 5ug of antibody; Lane 4 : Precipitated from PBS using 5ug of antibody. WB analysis was performed using bsm-50257M at 0.2ug/mL (1:5000) and incubated at 4℃ overnight, followed secondary antibody incubation for 60min at Room Temperature.
Lane 1 : A431 Cell Lysate; Lane 2 : HeLa Cell Lysate; Lane 3 : NIH3T3 Cell Lysate; Lane 4 : C6 Cell Lysate; probed with Bcl-2 (11C5) Monoclonal Antibody, unconjugated (bsm-50257M) at 1:10000 overnight at 4°C followed by a conjugated secondary antibody at 1:5000 for 60 minutes at Room Temperature.

Catalog #: bsm-50257M

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Immunoprecipitation of A431 cell lysates using Bid (21F10) Monoclonal Antibody (bsm-50265M): Lane 1 : A431 Cell Lysate; Lane 2 : Precipitated from 200ug of Cell Lysate using 2ug of antibody; Lane 3 : Precipitated from 200ug of Cell Lysate using 5ug of antibody; Lane 4 : Precipitated from PBS using 5ug of antibody. WB analysis was performed using bsm-50264M at 0.1ug/mL (1:10000) and incubated at 4℃ overnight, followed secondary antibody incubation for 60min at Room Temperature.
Lane 1 : HeLa Cell Lysate; Lane 2 : A431 Cell Lysate; Lane 3 : K562 Cell Lysate; Lane 4 : NCI-H460 Cell Lysate; probed with Bid (21F10) Monoclonal Antibody, unconjugated (bsm-50265M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody at 1:5000 for 60 minutes at Room Temperature.

Catalog #: bsm-50265M

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human lung carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Bid (8D2) monoclonal Antibody, Unconjugated (bsm-50264M) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
Raji probed with Bid (8D2) Monoclonal Antibody, Unconjugated (bsm-50264M) at 1:100 for 30 minutes followed by incubation with a FITC conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Lane 1 : HeLa Cell Lysate; Lane 2 : A431 Cell Lysate; Lane 3 : K562 Cell Lysate ; Lane 4 : NCI-H460 Cell Lysate; probed with Bid (8D2) Monoclonal Antibody, unconjugated (bsm-50264M) at 1:20000 overnight at 4°C followed by a conjugated secondary antibody at 1:5000 for 60 minutes at Room Temperature.
Immunoprecipitation of A431 cell lysates using Bid (8D2) Monoclonal Antibody (bsm-50264M): Lane 1 : A431 Cell Lysate; Lane 2 : Precipitated from 200ug of Cell Lysate using 2ug of antibody; Lane 3 : Precipitated from 200ug of Cell Lysate using 5ug of antibody; Lane 4 : Precipitated from PBS using 5ug of antibody. WB analysis was performed using bsm-50264M at 0.1ug/mL (1:10000) and incubated at 4℃ overnight, followed secondary antibody incubation for 60min at Room Temperature.

Catalog #: bsm-50264M

Applications: WB, IHC-P, IF(IHC-P), IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-50309M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1 : Calbindin-1 Recombinant Protein; Lane 2 : Calbindin-2 Recombinant Protein; probed with bsm-50359M Calbindin 1 (2E5) Monoclonal Antibody at 0.5ug/mL (1:2000) and incubated at 4℃ overnight, followed secondary antibody incubation for 60min at room temperature.
Lane 1 : IMR32 Cell Lysate; Lane 2 : Mouse Brain Tissue; Lane 3 : Rat Brain Tissue; probed with bsm-50359M Calbindin 1 (2E5) Monoclonal Antibody at 0.5ug/mL (1:2000) and incubated at 4℃ overnight, followed secondary antibody incubation for 60min at room temperature.

Catalog #: bsm-50359M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1 : K562 Cell Lysate; Lane 2 : 293T Cell Lysate; Lane 3 : PC12 Cell Lysate; Lane 4 : NIH3T3 Cell Lysate; probed with Casein Kinase II α (8E5) Monoclonal Antibody, unconjugated (bsm-50277M) at 1:500 overnight at 4°C followed by a conjugated secondary antibody at 1:5000 for 60 minutes at Room Temperature.
Lane 1 : Casein Kinase 1 alpha Recombinant Protein; Lane 2 : Casein Kinase 1 delta Recombinant Protein; Lane 3 : Casein Kinase 1 epsilon Recombinant Protein; Lane 4 : Casein Kinase 1 gamma Recombinant Protein; Lane 5 : Casein Kinase 2 alpha Recombinant Protein; Lane 6 : Casein Kinase 2 beta Recombinant Protein; lysates probed with Casein Kinase II α (8E5) Monoclonal Antibody, unconjugated (bsm-50277M) at 1:2000 overnight at 4°C followed by a conjugated secondary antibody at 1:5000 for 60 minutes at Room Temperature.
Immunoprecipitation of Casein Kinase II α from 293T cell lysates using Casein Kinase II α (8E5) Monoclonal Antibody (bsm-50277M): Lane 1 : 293T Cell Lysate; Lane 2 : Precipitated from 200ug of Cell Lysate using 2ug of antibody; Lane 3 : Precipitated from 200ug of Cell Lysate using 5ug of antibody; Lane 4 : Precipitated from PBS using 5ug of antibody. WB analysis was performed using bsm-50278M at 1.0ug/mL (1:1000) and incubated at 4℃ overnight, followed secondary antibody incubation for 60min at Room Temperature.

Catalog #: bsm-50277M

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse liver cell lysates; probed with CAT (626CT4.3.8) Monoclonal Antibody, unconjugated (bsm-51185M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
K562 cell lysates; probed with CAT (626CT4.3.8) Monoclonal Antibody, unconjugated (bsm-51185M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

Catalog #: bsm-51185M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #: bsm-50106M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Skin tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CD44 (Hermes-3) Monoclonal Antibody (bsm-51065M) at 1:25 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human lung adenocarcinoma section; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CD44 (Hermes-3) Monoclonal Antibody (bsm-51065M) at 1:25 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded SAMPLE tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CD44 (Hermes-3) Monoclonal Antibody (bsm-51065M) at 1:50 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #: bsm-51065M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-50311M

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-50310M

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: A431 Cell lysates; Lane 2: HeLa Cell lysates; Lane 3: K562 Cell lysates; Lane 4: MCF-7 Cell lysates; Lane 5: NIH/3T3 Cell lysates; probed with CDK4 (9C3) Monoclonal Antibody, unconjugated (bsm-51314M) at 1:2000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed, paraffin embedded human breast carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CDK4 (9C3) Monoclonal Antibody (bsm-51314M) at 1:25 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining

Catalog #: bsm-51314M

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #: bsm-50350M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-50301M

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-51099M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #: bsm-50349M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: HeLa Cell lysates; probed with CYLD (7C1) Monoclonal Antibody, unconjugated (bsm-51375M) at 1:4000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Hela probed with using CYLD (7C1) Monoclonal Antibody (green) unconjugated bsm-51375M at 1:25 for 60 minutes at 37ºC followed by secondary antibody at 1:200 compared to an isotype control of mouse IgG2a (blue).

Catalog #: bsm-51375M

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-51347M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #: bsm-51203M

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-50404M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-50272M

Applications: WB

Reactivity: Human, Rat

Conjugate: Unconjugated

Lane 1 : A431 Cell Lysate; Lane 2 : 293T Cell Lysate; Lane 3 : NIH3T3 Cell Lysate; probed with FAK1 (1D10) Monoclonal Antibody, unconjugated (bsm-50324M) at 1:500 overnight at 4°C followed by a conjugated secondary antibody at 1:5000 for 60 minutes at Room Temperature.
Immunoprecipitation of FAK1 from A431 cell lysates using FAK1 (1D10) Monoclonal Antibody (bsm-50324M): Lane 1 : A431 Cell Lysate; Lane 2 : Precipitated from 200ug of Cell Lysate using 2ug of antibody; Lane 3 : Precipitated from 200ug of Cell Lysate using 5ug of antibody; Lane 4 : Precipitated from PBS using 5ug of antibody. WB analysis was performed using bsm-50324M at 0.5ug/mL (1:2000) and incubated at 4℃ overnight, followed secondary antibody incubation for 60min at Room Temperature.

Catalog #: bsm-50324M

Applications: WB, IP

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #: bsm-50338M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: NIH/3T3 Cell lysates; Lane 2: RPMI8226 Cell lysates; Lane 3: NCI-H929 Cell lysates; Lane 4: mouse thymus lysates; probed with FGFR1OP2 (5C4) Monoclonal Antibody, unconjugated (bsm-51372M) at 1:4000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
U-2OS cells probed with using FGFR1OP2 (5C4) Monoclonal Antibody (green) unconjugated bsm-51372M at 1:25 for 60 minutes at 37ºC followed by secondary antibody at 1:200 compared to an isotype control of mouse IgG2a (blue).

Catalog #: bsm-51372M

Applications: WB, FCM

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #: bsm-50166M

Applications: IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-50124M

Applications: IF(ICC), IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-50353M

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-50354M

Applications: IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-50313M

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-50314M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1 : HeLa Cell Lysate; Lane 2 : 293T Cell Lysate; Lane 3 : SK-N-MC Cell Lysate; probed with MEK6 (MAP kinase kinase 6) (4G6) Monoclonal Antibody, unconjugated (bsm-50345M) at 1:500 overnight at 4°C followed by a conjugated secondary antibody at 1:5000 for 60 minutes at Room Temperature.
Lane 1 : MEK1 Recombinant Protein; Lane 2 : MEK2 Recombinant Protein; Lane 3 : MEK3 Recombinant Protein; Lane 4 : MEK4 Recombinant Protein; Lane 5 : MEK5 Recombinant Protein; Lane 6 : MEK6 Recombinant Protein; Lane 7 : MEK7 Recombinant Protein; probed with MEK6 (MAP kinase kinase 6) (AF4G6) Monoclonal Antibody, unconjugated (bsm-50345M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody at 1:5000 for 60 minutes at Room Temperature.

Catalog #: bsm-50345M

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-51315M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-50399M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-51414M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Sample Tissue/Cells lysates probed with antibodyname Monoclonal Antibody, unconjugated (bsm-51413M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at Room Temperature.
Paraformaldehyde-fixed, paraffin embedded Human heart tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with NME1 (1172CT2.4.1.1) Monoclonal Antibody (bsm-51413M) at 1:25 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #: bsm-51413M

Applications: WB, IHC-P

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #: bsm-50149M

Applications: WB, IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #: bsm-50127M

Applications: IP

Reactivity: Human

Conjugate: Unconjugated

Lane 1 : HeLa Cell Lysate; Lane 2 : HepG2 Cell Lysate; Lane 3 : MCF7 Cell Lysate; Lane 4 : Mouse Brain Tissue; Lane 5 : Rat Brain Tissue, probed with Peroxiredoxin V(12A) Monoclonal Antibody, unconjugated (bsm-50111M) at 1:2000 overnight at 4°C followed by a conjugated secondary antibody at 1:5000 for 60 minutes at Room Temperature.
Lane 1 : Prx1 Recombinant Protein; Lane 2 : Prx2 Recombinant Protein; Lane 3 : Prx3 Recombinant Protein; Lane 4 : Prx4 Recombinant Protein; Lane 5 : Prx5 Recombinant Protein; Lane 6 : Prx6 Recombinant Protein, probed with Peroxiredoxin V(12A) Monoclonal Antibody, unconjugated (bsm-50111M) at 1:2000 overnight at 4°C followed by a conjugated secondary antibody at 1:5000 for 60 minutes at Room Temperature.

Catalog #: bsm-50111M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded human liver tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with PGK1 (1086CT10.2.1) Monoclonal Antibody (bsm-51374M) at 1:25 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with PGK1 (1086CT10.2.1) Monoclonal Antibody (bsm-51374M) at 1:25 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #: bsm-51374M

Applications: WB, FCM, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1 : 293T Cell Lysate; Lane 2 : Mouse Brain Tissue; Lane 3 : Rat Brain Tissue lysates probed with PLC γ (1F1) Monoclonal Antibody, unconjugated (bsm-50132M) at 1:10000 overnight at 4°C followed by a conjugated secondary antibody at 1:5000 for 60 minutes at Room Temperature.
Immunoprecipitation of 293T cell lysates using Antibody Name\r\nMonoclonal Antibody (bsm-50132M): Lane 1 : 293T Cell Lysate; Lane 2 : Precipitated from 200ug of Cell Lysate using 2ug of antibody; Lane 3 : Precipitated from 200ug of Cell Lysate using 5ug of antibody; Lane 4 : Precipitated from PBS using 5ug of antibody. WB analysis was performed using bsm-50132M at 1.0ug/mL (1:1000) and incubated at 4℃ overnight, followed secondary antibody incubation for 60min at Room Temperature.

Catalog #: bsm-50132M

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-50133M

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: HeLa Cell lysates; Lane 2: K562 Cell lysates; Lane 3: Daudi Cell lysates; probed with PLIN3 (1C4) Monoclonal Antibody, unconjugated (bsm-51361M) at 1:2000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
HUVEC cells probed with using PLIN3 (1C4) Monoclonal Antibody (green) unconjugated bsm-51361M at 1:25 for 60 minutes at 37ºC followed by secondary antibody at 1:200 compared to an isotype control of mouse IgG1 (blue).
Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS cells; Antibody incubation with PLIN3 (1C4) Monoclonal Antibody, Unconjugated (bsm-51361M) at 1:25 overnight at 4°C, followed by a conjugated secondary (green). The nuclear counter stain is DAPI (blue).

Catalog #: bsm-51361M

Applications: WB, FCM, IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1 : A431 Cell Lysate; Lane 2 : MOLT4 Cell Lysate; Lane 3 : C6 Cell Lysate; Lane 4 : NIH3T3 Cell Lysate; probed with bsm-50383M PP1B (9B12) Monoclonal Antibody with 0.2ug/mL (1:5000) and incubated at 4℃ overnight, followed by secondary antibody incubation for 60min at room temperature.
Lane 1 : PP1A Recombinant Protein; Lane 2 : PP1B Recombinant Protein; Lane 3 : PP1G Recombinant Protein; probed with bsm-50383M PP1B (9B12) Monoclonal Antibody with 0.2ug/mL (1:5000) and incubated at 4℃ overnight, followed by secondary antibody incubation for 60min at room temperature.

Catalog #: bsm-50383M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1 : HeLa Cell Lysate; Lane 2 : 293T Cell Lysate; Lane 3 : C6 Cell Lysate; Lane 4 : NIH3T3 Cell Lysate; probed bsm-50397M PP1G (11F10) Monoclonal Antibody with 0.1ug/mL (1:10000) dilution and incubated at 4℃ overnight, followed by secondary antibody incubation for 60min at room temperature.
Lane 1 : PP1A Recombinant Protein; Lane 2 : PP1B Recombinant Protein; Lane 3 : PP1G Recombinant Protein; probed bsm-50397M PP1G (11F10) Monoclonal Antibody with 0.1ug/mL (1:10000) dilution and incubated at 4℃ overnight, followed by secondary antibody incubation for 60min at room temperature.

Catalog #: bsm-50397M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: 293; Lane 2: Hela whole cell lysate at 20 µg per lane; probed with bsm-51055M PRKAA2 (206CT5.4.2) Monoclonal Antibody at 1:1000 dilution and 4℃ overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), incubated with PRKAA2 (206CT5.4.2) Monoclonal Antibody (bsm-51055M) at 1:25 dilution for 1 h at 37℃, followed by secondary antibody incubation (green), actin (red), and nuclear counter stain with DAPI (blue).

Catalog #: bsm-51055M

Applications: WB, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-50134M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-50356M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated