Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch

Primary Antibodies

Collection Menu
Paraformaldehyde-fixed, paraffin embedded Human stomach tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with RAC1 (13C4) Monoclonal Antibody (bsm-51282M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human skin tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with RAC1 (13C4) Monoclonal Antibody (bsm-51282M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: A431; Lane 2: NIH\/3T3; Lane 3: C6 cell lysate probed with RAC1 (13C4) Monoclonal Antibody (bsm-51282M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #: bsm-51282M

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeled with ATG4A (1458CT808.66.25.69) Monoclonal Antibody (bsm-51292M) at 1\/25 dilution, followed by secondary antibody (green), cytoplasmic actin staining (red), and nuclear counter stain with DAPI (blue).
Paraformaldehyde-fixed, paraffin embedded Human brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ATG4A (1458CT808.66.25.69) Monoclonal Antibody (bsm-51292M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
K562 lysates probed with ATG4A (14C9) Monoclonal Antibody, Unconjugated (bsm-51292M) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Catalog #: bsm-51292M

Applications: WB, IHC-P, IF(ICC), IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: MCF-7 cell lysates; probed with DDR1 (4C4) Monoclonal Antibody, unconjugated (bsm-51295M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with DDR1 (4C4) Monoclonal Antibody (bsm-51295M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #: bsm-51295M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeled with DENR (2C9) Monoclonal Antibody (bsm-51318M) at 1\/25 dilution, followed by secondary antibody (green), cytoplasmic actin staining (red), and nuclear counter stain with DAPI (blue).
Paraformaldehyde-fixed, paraffin embedded human skeletal muscle tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with DENR (2C9) Monoclonal Antibody (bsm-51318M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: A431; Lane 2: human skeletal muscle lysates; Lane 3: human brain Cell lysates; probed with DENR (2C9) Polyclonal Antibody, unconjugated (bsm-51318M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Hela cells probed with using DENR (2C9) Monoclonal Antibody bsm-51318M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:400 (green), compared to an isotype control of mouse IgG2b (blue).

Catalog #: bsm-51318M

Applications: WB, FCM, IHC-P, IF(ICC), IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: A431 Cell lysates; Lane 2: HL-60 Cell lysates; Lane 3: U-2OS Cell lysates; probed with RAB5C (6C1) Monoclonal Antibody, unconjugated (bsm-51343M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
U-2OS cells probed with using RAB5C (6C1) Monoclonal Antibod bsm-51343M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody (green) at 1:400 compared to an isotype control of mouse IgG2b (blue).

Catalog #: bsm-51343M

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Lane 1: MOLT-4 Cell lysates; probed with ETS1 (1C1) Monoclonal Antibody, unconjugated (bsm-51344M) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
K562 cells probed with using ETS1 (1C1) Monoclonal Antibody (green) unconjugated bsm-51344M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:400 compared to an isotype control of mouse IgG2b (blue).

Catalog #: bsm-51344M

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Jurkat; Lane 2: A431 cell lysate at 20 \u00b5g per lane, probed with bsm-51358M SRC (2C2) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Lane 1: A549 cell lysates probed with SRC (2C2) Monoclonal Antibody, Unconjugated (bsm-51358M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #: bsm-51358M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded human skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with MYO18B (3C5) Monoclonal Antibody (bsm-51376M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human heart tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with MYO18B (3C5) Monoclonal Antibody (bsm-51376M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
A431 cells probed with using MYO18B (3C5) Monoclonal Antibody (green) unconjugated bsm-51376M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:200 compared to an isotype control of mouse IgG2b (blue).
Recombinant protein lysates probed with MYO18B (3C5) Polyclonal Antibody, unconjugated (bsm-51376M) at 1:16000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #: bsm-51376M

Applications: WB, FCM, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-51423M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-51386M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-51440M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: human brain lysate; Lane 2: human cerebellum lysate at 20 \u00b5g per lane, probed with bsm-51047M GFAP (183CT3.1.5) Monoclonal Antibody at 1:4000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Lane 1: MCF-7 Cell lysates; probed with GFAP (183CT3.1.5) Monoclonal Antibody, unconjugated (bsm-51047M) at 1:4000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #: bsm-51047M

Applications: WB, IHC-P

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #: bsm-51087M

Applications: WB, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-51126M

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #: bsm-51163M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-51207M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: MCF-7; Lane 2: A431; Lane 3: HeLa; Lane 4: HepG2; Lane 5: NIH\/3T3; Lane 6: mouse stomach; Lane 7: mouse liver lysate at 35 \u00b5g per lane. probed with bsm-51216M ENO1 (7C4) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ENO1 (7C4) Monoclonal Antibody (bsm-51216M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #: bsm-51216M

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: CEM Cell lysates; Lane 2: 293; Lane 3: C6 probed with bsm-51241M SUMO2 (7A3) Monoclonal Antibody at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
HeLa cells stained with SUMO2 (7A3) Monoclonal Antibody (bsm-51241M) at 1:25 dilution, followed by Alexa Fluor 488-conjugated secondary antibody (green), cytoplasmic actin (red).

Catalog #: bsm-51241M

Applications: WB, IF(ICC), IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Lane 1: Mouse brain lysates; Lane 2: HeLa Cell lysates; Lane 3: Y79 Cell lysates; Lane 4: CEM Cell lysates; Lane 5: PC-12 Cell lysates; probed with TBB5 (7D3) Monoclonal Antibody, unconjugated (bsm-51387M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human testis tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with TBB5 (7D3) Monoclonal Antibody (bsm-51387M) at 1:50 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
A549 cells stained with TBB5 (7D3) Monoclonal Antibody (bsm-51387M) at 1:25 dilution, followed by Alexa Fluor 488-conjugated secondary antibody (green), cytoplasmic actin (red).

Catalog #: bsm-51387M

Applications: WB, IHC-P, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: HepG2 Cell lysates; Lane 2: Rat Liver lysates; Lane 3: MCF-7 Cell lysates; Lane 4: NIH\/3T3 Cell lysates; probed with PHB (12C6) Monoclonal Antibody, unconjugated (bsm-51395M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human stomach tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with PHB (12C6) Monoclonal Antibody (bsm-51395M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #: bsm-51395M

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: A431 Cell lysates; Lane 2: HepG2 Cell lysates; Lane 3: NIH\/3T3 Cell lysates; Lane 4: mouse brain lysates; Lane 5: C6 Cell lysates; Lane 6: PC-12 Cell lysates; probed with YWHAZ (1B6) Monoclonal Antibody, unconjugated (bsm-51397M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with YWHAZ (1B6) Monoclonal Antibody, Unconjugated (bsm-51397M) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #: bsm-51397M

Applications: WB, IHC-P, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: K562 Cell lysates; Lane 2: mouse brain lysates; Lane 3: U-251 MG Cell lysates; probed with BAIAP2 (1037CT15.1.3) Monoclonal Antibody, unconjugated (bsm-51410M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Cerebrum lysates probed with BAIAP2 (4G7) Monoclonal Antibody, Unconjugated (bsm-51410M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #: bsm-51410M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: A549 Cell lysates; Lane 2: SK-BR-3 Cell lysates; Lane 3: C2C12 Cell lysates; probed with CAPN2 (1C1) Monoclonal Antibody, unconjugated (bsm-51429M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human stomach tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CAPN2 (1C1) Monoclonal Antibody (bsm-51429M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Flow cytometric analysis of U-87 MG cells using bsm-51429M CAPN2 Antibody (green) compared to mouse IgG2b isotype control (blue). bsm-51429M was diluted at 1:100 dilution. Alexa Fluor\u00ae 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.

Catalog #: bsm-51429M

Applications: WB, FCM, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Jurkat cell lysate probed with BCL-2 (2C4) Monoclonal Antibody, Unconjugated (bsm-52022M) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Paraformaldehyde-fixed, paraffin embedded human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal serum) at 37\u00b0C for 20min; Antibody incubation with BCL-2 (2C4) Monoclonal Antibody (bsm-52022M) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #: bsm-52022M

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Histone extracts (15 \u03bcg) from HeLa cells were analysed by Western blot using H3K4un (3C5) Monoclonal Antibody (Cat. No. bsm-53012M) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
HeLa cells were stained with H3K4un (3C5) Monoclonal Antibody (Cat. No. bsm-53012M) and with DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K4un antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
To test the specificity an ELISA was performed using a serial dilution of H3K4un (3C5) Monoclonal Antibody (Cat. No. bsm-53012M). The wells were coated with peptides containing the unmodified H3K4 region as well as the mono-, di- and trimethylated H3K4. Figure 2 shows a high specificity of the antibody for the unmodified peptide.
ChIP assays were performed using HeLa cells, the monoclonal antibody against H3K4un (Cat. No. bsm-53012M) and optimized PCR primer sets for qPCR. A titration of the antibody consisting of 1, 5 and 10 \u03bcg per ChIP experiment was analysed. IgG (5 \u03bcg\/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH promoter and for exon 2 of the myoglobin gene. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Catalog #: bsm-53012M

Applications: WB, ELISA, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Histone extracts (15 \u03bcg) from HeLa cells were analysed by Western blot using the H3K4me1 Monoclonal Antibody (bsm-53013M) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the molecular weight marker (in kDa) is shown on the left.
HeLa cells were stained with H3K4me1 (34A1) Monoclonal Antibody (Cat. No. bsm-53013M) and with DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 5% normal goat serum and 1% BSA. The cells were labelled with the H3K4me1 antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge is shown on the right.
To test the specificity an ELISA was performed using a serial dilution of H3K4me1 (34A1) Monoclonal Antibody (Cat. No. bsm-53013M). The wells were coated with peptides containing the unmodified H3K4 as well as the mono-, di- and trimethylated H3K4 and the monomethylated H3K9. Figure shows a high specificity of the antibody for the modification of interest.
ChIP assays were performed using HeLa cells, H3K4me1 (34A1) Monoclonal Antibody (bsm-53013M) and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1.6 million cells. A titration of the antibody consisting of 1, 5 and 10 \u03bcg per ChIP experiment was analyzed. IgG (5 \u03bcg\/IP) was used as negative IP control. QPCR was performed with primers for the promoter and the coding region of the GAPDH gene, and for the RPL10 and HBB promoters. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Catalog #: bsm-53013M

Applications: WB, ELISA, IHC-P, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-53000M

Applications: WB, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-49132M

Applications: WB, ELISA

Reactivity: Virus, Others, SARS

Conjugate: Unconjugated

Catalog #: bsm-49131M-HRP

Applications: WB

Reactivity: Virus, Others, SARS

Conjugate: HRP

Catalog #: bsm-49133M

Applications: WB, ELISA

Reactivity: Virus, Others, SARS

Conjugate: Unconjugated

Catalog #: bsm-49131M

Applications: WB, ELISA

Reactivity: Virus, Others, SARS

Conjugate: Unconjugated

Catalog #: bsm-49133M-HRP

Applications: WB

Reactivity: Virus, Others, SARS

Conjugate: HRP

Catalog #: bsm-41411M

Applications: WB, ELISA, IHC-P

Reactivity: Virus

Conjugate: Unconjugated

Catalog #: bsm-49134M

Applications: WB, ELISA

Reactivity: Virus, Others, SARS

Conjugate: Unconjugated

Catalog #: bsm-41413M

Applications: WB, ELISA, FCM

Reactivity: Virus

Conjugate: Unconjugated

Catalog #: bsm-41412M

Applications: WB, ELISA

Reactivity: Virus

Conjugate: Unconjugated

Catalog #: bsm-49135M

Applications: WB, ELISA

Reactivity: Virus, Others, SARS

Conjugate: Unconjugated

Catalog #: bsm-49133M-Biotin

Applications: WB

Reactivity: Virus, Others, SARS

Conjugate: Biotin

Catalog #: bsm-49131M-Biotin

Applications: WB

Reactivity: Virus, Others, SARS

Conjugate: Biotin

Catalog #: bsm-41414M

Applications: WB, ELISA

Reactivity: Virus

Conjugate: Unconjugated

Catalog #: bsm-41415M

Applications: WB, ELISA, IHC-P

Reactivity: Virus

Conjugate: Unconjugated

Lane 1: Human Brain tissue lysates; Lane 2: Mouse Brain tissue lysates probed with CRMP2 Monoclonal Antibody, Unconjugated (bsm-51453M) at 1:8000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse NIH\/3T3 cell lysates; Lane 2: Mouse Brain tissue lysates probed with CRMP2 Monoclonal Antibody, Unconjugated (bsm-51453M) at 1:12800 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #: bsm-51453M

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #: bsm-51504M

Applications: WB

Reactivity:

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Calpain 2 Monoclonal Antibody, Unconjugated (bsm-51592M) at 1:25 overnight at 4\u00b0C, DAB staining.
Lane 1: A549 cell lysates; Lane 2: A431 cell lysates; Lane 3: SK-BR-3 cell lysates probed with Calpain 2 Monoclonal Antibody, Unconjugated (bsm-51592M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #: bsm-51592M

Applications: WB, IHC-P

Reactivity:

Conjugate: Unconjugated

Catalog #: bsm-41411M-HRP

Applications: WB, IHC-P

Reactivity: Virus

Conjugate: HRP

Catalog #: bsm-41411M-Biotin

Applications: WB, IHC-P

Reactivity: Virus

Conjugate: Biotin

Catalog #: bs-51013R

Applications: WB, ELISA, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bs-51012R

Applications: WB, ELISA, IF

Reactivity: Human

Conjugate: Unconjugated