RECEIVE 25% OFF PUBLISHED ANTIBODIES WITH CODE "PUB25"! Offer valid for US customers and available internationally through distributors.

Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch

Primary Antibodies

Primary Antibodies

Our Primary Antibodies are research-use antibodies designed to support protein detection and target analysis across a wide range of applications and species. Our collection includes recombinant antibodies, monoclonal antibodies, and polyclonal antibodies for use in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), flow cytometry (FC), ELISA, and other common applications.

These primary antibodies are available for targets involved in cancer research, immunology, neuroscience, cell biology, metabolism, apoptosis, signaling pathways, and disease-related research. Bioss offers primary antibodies guaranteed for use in human, mouse, rat, and other species to help researchers choose the right antibody format for their experimental model and detection method.



×

Collection Menu

Catalog #1: bs-2843R-PerCP-Cy5.5

Applications: WB, IF

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bs-2843R-RBITC

Applications: WB, IF

Reactivity: Human

Conjugate: RBITC

Catalog #1: bs-2843R-PE-Cy5

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5

Catalog #1: bs-2843R-BF750

Applications: WB, IF

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bs-2843R-APC-Cy5.5

Applications: WB, IF

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bs-2843R-PE-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bsm-60645r

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bsm-60654r

Applications: WB, IHC-P, IHC-F, IF

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60659r-100ul

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60659r-cy5-100ul

Applications: WB

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-60659r-Cy5.5-100ul

Applications: WB

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-60659r-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-60659r-fitc-100ul

Applications: WB

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-60659r-pe-100ul

Applications: WB

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-60659r-biotin-100ul

Applications: WB

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-60659r-hrp-100ul

Applications: WB

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-60659r-cy3-100ul

Applications: WB

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-60659r-bf350-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-60659r-bf405-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-60659r-bf488-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-60659r-bf555-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-60659r-bf594-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bsm-60659r-bf647-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bsm-60659r-bf680-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bsm-60659r-bf750-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bsm-60659r-apc-100ul

Applications: WB

Reactivity: Human

Conjugate: APC

Catalog #1: bsm-60659r-apc-Cy5.5-100ul

Applications: WB

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bsm-60659r-apc-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: APC-Cy7

Catalog #1: bsm-60659r-pe-cy5-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5

Catalog #1: bsm-60659r-pe-Cy5.5-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bsm-60659r-percp-100ul

Applications: WB

Reactivity: Human

Conjugate: PerCP

Catalog #1: bsm-60659r-percp-Cy5.5-100ul

Applications: WB

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-60659r-rbitc-100ul

Applications: WB

Reactivity: Human

Conjugate: RBITC

Catalog #1: bsm-60659r-pe-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bsm-60661r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60662r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60678r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60679r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:2000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: (-): HeLa, (+): HeLa+ Human \u03b1-TNF \r\n(20ng\/ml, 30min)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 36 kDa\r\nObserved MW: 36 kDa
Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:2000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: (-): C6, (+): C6+ Calyculin A (100nM, \r\n30min)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 36 kDa\r\nObserved MW: 36 kDa
Tissue: (A) Rat kidney, (B) Rat kidney, then the tissue was incubated with alkaline phosphatase (50 \r\nU\/ml) at 37\u2103 for 1 hour\r\nSection type: Formalin-fixed & Paraffin-embedded section \r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at room temperature \r\nSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60711R
Tissue: (A) Mouse kidney, (B) Mouse kidney, then the tissue was incubated with alkaline \r\nphosphatase (50 U\/ml) at 37\u2103 for 1 hour\r\nSection type: Formalin-fixed & Paraffin-embedded section \r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at room temperature \r\nSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60711R

Catalog #1: bsm-60711r

Applications: WB, IHC-P, IHC-F, IF

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60717r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-52204r

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

The Hela ( treated with 200J\/m2 UV-C then recovery for 30 min) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature.Primary Antibody (green):Rabbit Anti-p-Hsp27 (S78) antibody (bsm-52167R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Hela (H) cells were treated with or without UV (40 mJ\/cm2) for 30 min and anisomycin (25 \u03bcg\/mL) for 30 min , 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with Phospho-HSP27 (Ser78) monoclonal antibody, unconjugated (bsm-52167R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-HSP27 (Ser78) Monoclonal Antibody, Unconjugated (bsm-52167R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-HSP27 (Ser78) Monoclonal Antibody, Unconjugated (bsm-52167R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52167r

Applications: WB, ELISA, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-10940R

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-10940R-Cy3

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-10940R-Cy5.5

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-10940R-Cy5

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-10940R-Cy7

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-10940R-FITC

Applications: WB

Reactivity: Mouse, Rat

Conjugate: FITC