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Unconjugated Primary Antibodies



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Catalog #1: v1105-1mg

Applications: ELISA, LFA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: v13302-1mg

Applications: ELISA, LFA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: v0706-1mg

Applications: ELISA, LFA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: v0505-1mg

Applications: ELISA, LFA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: v1201-1mg

Applications: ELISA, LFA

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Mouse intestinal lysates Lane 2: mouse testicular lysates probed with Rabbit Anti-Cdc25C Polyclonal Antibody, Unconjugated (bs-9597R) at 1:300 overnight at 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.
Mouse splenocytes probed with Rabbit Anti-Cdc25C Polyclonal Antibody, Unconjugated (bs-9597R) (green) at 1:50 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only(light blue), and isotype control(orange).
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Cdc25C) polyclonal Antibody, Unconjugated (bs-9597R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Cdc25C Polyclonal Antibody(bs-9597R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-9597r-100l

Applications: IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3574r-100l

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-2229r-100l

Applications: ELISA

Reactivity: Mouse, Bacteria

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human gastric cancer labeled with Anti-phospho-AKT3 (Ser472)Polyclonal Antibody, Unconjugated (bs-5209R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-phospho-AKT3 (Ser472)Polyclonal Antibody, Unconjugated (bs-5209R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3) used at 1:200 dilution for 40 minutes at 37\u00b0C and DAPI staining
Lane 1: Human HUVEC cell lysates; Lane 2: Human A431 cell lysates probed with AKT1\/2\/3 Polyclonal Antibody, Unconjugated (bs-5209R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AKT1\/2\/3 (Ser472\/Ser473\/Ser474) Polyclonal Antibody, Unconjugated (bs-5209R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-5209r-100l

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Human A549 probed with Rabbit Anti-CD80 Polyclonal Antibody, Unconjugated (bs-1479R) at 1:300 overnight at 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at RT.
Lane 1: Mouse RAW264.7 cell lysates; Lane 2: Mouse Sp2\/0 cell lysates; Lane 3: Rat Thymus lysates; Lane 4: Human Raji cell lysates; Lane 5: Human K562 cell lysates probed with CD80 Polyclonal Antibody, Unconjugated (bs-1479R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Raji cells were\u00a0 incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with CD80 Polyclonal Antibody(bs-1479R)at 1:50 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-1479r-100l

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0605r-100l

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rabbit

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-NR2B\/NMDAR2B Polyclonal Antibody, Unconjugated (bs-0222R) at 1:300, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded: : rat brain tissue labeled with Anti-NR2B\/NMDAR2B Polyclonal Antibody, Unconjugated (bs-0222R) at 1:200,, followed by conjugation to the secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC) at 1:200 for 40 minutes at 37\u00b0C and DAPI staining
Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-NR2B\/NMDAR2B Polyclonal Antibody, Unconjugated (bs-0222R) at 1:200,, followed by conjugation to the secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC) at 1:200 for 40 minutes at 37\u00b0C
A549 lysates probed with NMDAR2B Polyclonal Antibody, Unconjugated (bs-0222R) at 1:300 overnight at 4\u02daC. Followed by a conjugated secondary antibody at 1:10000 for 60 min at 37\u02daC.

Catalog #1: bs-0222r-100l

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Neuroblastoma cells labeled with Anti-NSE\/ENO2\/\u03b3 Enolase Polyclonal Antibody, Unconjugated (bs-1027R) at 1:200, followed by conjugation to the secondary antibody
Formalin-fixed and paraffin embedded dog bladder tissue labeled with Anti-NSE\/ENO2\/\u03b3 Enolase Polyclonal Antibody, Unconjugated (bs-1027R) at 1:800, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NSE Polyclonal Antibody, Unconjugated (bs-1027R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
A431 lysates probed with NSE Polyclonal Antibody, Unconjugated (bs-1027R) at 1:300 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37\u02daC.

Catalog #1: bs-1027r-100l

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: Unconjugated

Catalog #1: bsm-77002m-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-63313r-100ul

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-63309r-100ul

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-63287r-100ul

Applications: WB, IHC-P, IHC-F, IP, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-63246r-100ul

Applications: WB, IP, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-63207r-100ul

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-63198r-100ul

Applications: WB, IHC-P, IHC-F, IP, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-63160r-100ul

Applications: WB, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-63155r-100ul

Applications: WB, IHC-P, IHC-F, IP, IF, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-63152r-100ul

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-63145r-100ul

Applications: WB, IHC-P, IHC-F, IP, IF, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-63121r-100ul

Applications: WB, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-63111r-100ul

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-63102r-100ul

Applications: WB, IHC-P, IHC-F, IP, IF, Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-63095r-100ul

Applications: WB, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-63030r-100ul

Applications: WB, IHC-P, IHC-F, IP, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-55666r-100ul

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-55667m-100ul

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

4% Paraformaldehyde-fixed HT-29 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (E cadherin) monoclonal Antibody, unconjugated (bsm-60971R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed T-47D (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (E cadherin) monoclonal Antibody, unconjugated (bsm-60971R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The T-47D (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.) , followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-E cadherin antibody (bsm-60971R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-60971r-100ul

Applications: Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-55625r-100ul

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bsm-55612r-100ul

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-55616r-100ul

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-55614r-100ul

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-55615r-100ul

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-55613m-100ul

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-55611r-100ul

Applications: IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-55032r-100ul

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-54806r-100ul

Applications: IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-43716m-100ul

Applications: IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-31228m-100ul

Applications: IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-25816r-100ul

Applications: Flow-Cyt

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-25885r-100ul

Applications: ELISA, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-16696r-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Pig

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with PRDX2 monoclonal antibody, unconjugated (bsm-52128R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PRDX2 Monoclonal Antibody, Unconjugated (bsm-52128R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PRDX2 Monoclonal Antibody, Unconjugated (bsm-52128R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PRDX2 Monoclonal Antibody, Unconjugated (bsm-52128R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52128r-100ul

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-9843r-100ul

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated