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Western Blot Antibodies



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Catalog #1: bs-0025M

Applications: WB, ELISA, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0293M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Rat

Conjugate: HRP

Catalog #1: bs-0369M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Rabbit

Conjugate: HRP

Catalog #1: bs-0358M

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Guinea Pig

Conjugate: Unconjugated

Catalog #1: bs-0296P-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded (human thyroid gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Procalcitonin (PCT)) Monoclonal Antibody, Unconjugated (bsm-41284M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Thyroid Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Procalcitonin (PCT) Monoclonal Antibody, Unconjugated (bsm-41284M) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Mouse IgG antibody (Green, bs-0296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bsm-41284m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-70160M

Applications: WB, IHC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-43534m-pe-cy7-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Rat

Conjugate: PE-Cy7

Catalog #1: bsm-43017m-biotin-100ul

Applications: WB

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-33185M-BF350

Applications: WB

Reactivity: Species independent

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33207M-BF405

Applications: WB

Reactivity: Rat

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33085m-bf488-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33062M-PE-Cy7

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bsm-33052m-percp-Cy5.5-100ul

Applications: WB, FCM

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-2235M-HRP

Applications: WB, ELISA

Reactivity: Human

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded (human placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (PD-L1) Monoclonal Antibody, Unconjugated (bsm-54472R) at 1200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (PD-L1) Monoclonal Antibody, Unconjugated (bsm-54472R) at 1200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (PD-L1) Monoclonal Antibody, Unconjugated (bsm-54472R) at 1200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with PD-L1 monoclonal antibody, unconjugated (bsm-54472R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-54472r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51583m

Applications: WB, IHC-P, IHC-F, IF

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-51463m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-0966M-Cy5

Applications: WB, IF(ICC)

Reactivity: Tags

Conjugate: Cy5

Paraformaldehyde-fixed, paraffin embedded (rat placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Cyclophilin B ) Monoclonal Antibody, Unconjugated (bsm-33228M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Cyclophilin B ) Monoclonal Antibody, Unconjugated (bsm-33228M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Cyclophilin B ) Monoclonal Antibody, Unconjugated (bsm-33228M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Cyclophilin B ) Monoclonal Antibody, Unconjugated (bsm-33228M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33228m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33201M-Biotin

Applications: WB

Reactivity: Goat, Cow

Conjugate: Biotin

Catalog #1: bsm-33008m-hrp-100ul

Applications: WB

Reactivity: Species independent

Conjugate: HRP

Catalog #1: bsm-33008m

Applications: WB

Reactivity: Species independent

Conjugate: Unconjugated

Catalog #1: bsm-33209M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33057m-cy7-100ul

Applications: WB, IF(IHC-P), IF(ICC), IF

Reactivity: Human, Rat

Conjugate: Cy7

Catalog #1: bsm-33051m-cy5-100ul

Applications: WB, IF(IHC-P), IF(ICC), IF

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-33007M-HRP

Applications: WB, ELISA

Reactivity: Species independent

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LC3A Monoclonal Antibody, Unconjugated (bsm-33309M) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LC3A Monoclonal Antibody, Unconjugated (bsm-33309M) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (LC3A ) Monoclonal Antibody, Unconjugated (bsm-33309M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (LC3A ) Monoclonal Antibody, Unconjugated (bsm-33309M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33309m

Applications: WB, IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CRP (C11F2) Monoclonal Antibody, Unconjugated (bsm-0391M) at 1:400 overnight at 4\u00b0C, followed secondary and DAB staining.
Lane 1: C Reactive Protein (CRP); 40ug loaded into the lane; Probed with CRP (C11F2) Monoclonal Antibody, unconjugated (bsm-0391M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: C Reactive Protein(CRP) probed with CRP(C11F2) Monoclona Antibody, Unconjugated (bsm-0391M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human U-2OS cell lysates; Lane 2: Human Siha cell lysates probed with CRP(C11F2) Monoclonal Antibody, Unconjugated bsm-0391MR) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-0391M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-41268M

Applications: WB, ELISA

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41267M-Biotin

Applications: WB, ELISA

Reactivity: SARS-CoV-2

Conjugate: Biotin

Catalog #1: bsm-33166M-Biotin

Applications: WB, ELISA

Reactivity: Tags, Others

Conjugate: Biotin

Catalog #1: bsm-33157m

Applications: WB

Reactivity: Species independent

Conjugate: Unconjugated

Catalog #1: bsm-33175M

Applications: WB

Reactivity: Species independent

Conjugate: Unconjugated

Catalog #1: bsm-49132m

Applications: WB, ELISA

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta-Actin Monoclonal Antibody (bsm-33036M-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta-Actin Monoclonal Antibody (bsm-33036M-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta-Actin Monoclonal Antibody (bsm-33036M-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta-Actin Monoclonal Antibody (bsm-33036M-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.

Catalog #1: bsm-33036m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: HRP

Histone extracts (15 \u03bcg) from HeLa cells were analysed by Western blot using H3K27me3 (9B7) Monoclonal Antibody (bsm-53019M) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
HeLa cells were stained with H3K27me3 (9B7) Monoclonal Antibody (bsm-53019M) and DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K27me3 antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse secondary antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
To test the specificity an ELISA was performed using a serial dilution of H3K27me3 (9B7) Monoclonal Antibody (bsm-53019M). The wells were coated with peptides containing the unmodified H3K27 region as well as the mono-, di- and trimethylated H3K27 and the trimethylated H3K9. Figure shows a high specificity of the antibody for the peptide containing the modification of interest.
ChIP assays were performed using HeLa cells, H3K27me3 (9B7) Monoclonal Antibody (bsm-53019M) and optimized PCR primer sets for qPCR. ChIP was performed on sheared chromatin from 1 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 \u03bcg per ChIP experiment was analysed. IgG (2 \u03bcg\/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the active genes c-fos and GAPDH as negative controls, and for the coding regions of the inactive genes MYT1 and TSH2B as positive controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K27me3 is preferably present at inactive genes.

Catalog #1: bsm-53019M

Applications: WB, ELISA, IF(ICC), IF

Reactivity: Human

Conjugate: Unconjugated

Histone extracts (15 \u03bcg) from HeLa cells were analysed by Western blot using the H3K4me1 Monoclonal Antibody (bsm-53013M) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the molecular weight marker (in kDa) is shown on the left.
HeLa cells were stained with H3K4me1 (34A1) Monoclonal Antibody (Cat. No. bsm-53013M) and with DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 5% normal goat serum and 1% BSA. The cells were labelled with the H3K4me1 antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge is shown on the right.
To test the specificity an ELISA was performed using a serial dilution of H3K4me1 (34A1) Monoclonal Antibody (Cat. No. bsm-53013M). The wells were coated with peptides containing the unmodified H3K4 as well as the mono-, di- and trimethylated H3K4 and the monomethylated H3K9. Figure shows a high specificity of the antibody for the modification of interest.
ChIP assays were performed using HeLa cells, H3K4me1 (34A1) Monoclonal Antibody (bsm-53013M) and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1.6 million cells. A titration of the antibody consisting of 1, 5 and 10 \u03bcg per ChIP experiment was analyzed. IgG (5 \u03bcg\/IP) was used as negative IP control. QPCR was performed with primers for the promoter and the coding region of the GAPDH gene, and for the RPL10 and HBB promoters. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Catalog #1: bsm-53013M

Applications: WB, ELISA, IHC-P, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33114m

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane1: HeLa lysates; Lane 2: HepG2 lysates; Lane 3: mouse placenta lysates probed with AMPK alpha-1 Polyclonal Antibody, Unconjugated (bs-33338M) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AMPK alpha-1 Monoclonal Antibody, Unconjugated (bsm-33338M) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Rat Liver lysates; Lane 2: Human Hela cell lysates; Lane 3: Human HepG2 cell lysates probed with AMPK alpha-1 Monoclonal Antibody, Unconjugated (bsm-33338M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
A549 cell; 4% Paraformaldehyde-fixed; Ice-cold methanol at -20\u2103 for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (AMPK alpha-1) monoclonal Antibody, Unconjugated (bsm-33338M) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bsm-33338M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Hela lysates; Lane 2: MDA-MB-231 lysates probed with CK7 Polyclonal Antibody, Unconjugated (bs-33060M) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CK7 Monoclonal Antibody, Unconjugated (bsm-33060M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CK7 Monoclonal Antibody, Unconjugated (bsm-33060M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CK7 Monoclonal Antibody, Unconjugated (bsm-33060M) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-33060m

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33143m-fitc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: FITC

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with STAT3 (3F5) Monoclonal Antibody, Unconjugated (bsm-33218M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with STAT3 (3F5) Monoclonal Antibody, Unconjugated (bsm-33218M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with STAT3 (3F5) Monoclonal Antibody, Unconjugated (bsm-33218M) at 1:2000 overnight at 4\u00b0C, DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with STAT3 monoclonal antibody, unconjugated (bsm-33218M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-33218m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33033m-biotin-100ul

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: Biotin

Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: alpha smooth muscle Actin Monoclonal Antibody, conjugated (bsm-33187M-FITC) at 1:200 overnight at 4\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: alpha smooth muscle Actin Monoclonal Antibody, conjugated (bsm-33187M-FITC) at 1:200 overnight at 4\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: alpha smooth muscle Actin Monoclonal Antibody, conjugated (bsm-33187M-FITC) at 1:200 overnight at 4\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: alpha smooth muscle Actin Monoclonal Antibody, conjugated (bsm-33187M-FITC) at 1:200 overnight at 4\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bsm-33187m-fitc-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: FITC

25 ug total protein per lane of various lysates (see on figure) probed with Histone H3\/HIST3H3\/HRP monoclonal antibody, conjugated (bsm-33042M-HRP) at 1:50000 dilution and 4\u00b0C overnight incubation.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H3 Monoclonal Antibody (bsm-33042M-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H3 Monoclonal Antibody (bsm-33042M-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H3 Monoclonal Antibody (bsm-33042M-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.

Catalog #1: bsm-33042m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Formalin-fixed and paraffin embedded rat kidney labeled with Cytochrome C (6B10) Monoclonal Antibody, Unconjugated (bsm-33193M) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytochrome C (6B10) Monoclonal Antibody, Unconjugated (bsm-33193M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytochrome C (6B10) Monoclonal Antibody, Unconjugated (bsm-33193M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytochrome C (6B10) Monoclonal Antibody, Unconjugated (bsm-33193M) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-33193m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Rat, Rabbit

Conjugate: Unconjugated

Catalog #1: bsm-10846m-hrp-100ul

Applications: WB

Reactivity: Human

Conjugate: HRP