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Primary Antibodies

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Lane 1: NIH/3T3 lysates; Lane 2: Hela lysates probed with STAT6 (9A7) Monoclonal Antibody (bsm-52239R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Hela cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS and stained with STAT6 (9A7) Monoclonal Antibody (bsm-52239R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
HepG2 cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS and stained with STAT6 (9A7) Monoclonal Antibody (bsm-52239R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with STAT6 (9A7) Monoclonal Antibody at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.

Catalog #: bsm-52239R

Applications: WB, FCM, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with ER alpha (5G7) Monoclonal Antibody (bsm-53007M) at a concentration of 7 μg/ ml. Figure shows that the antibody is specific for ER alpha isoform, whereas the ER beta isoform is not recognized.
COS-7 cells transiently over-expressing human ERα (left) or ERß1 (right) were labeled with the ER alpha (5G7) Monoclonal Antibody (bsm-53007M), used at a concentration of 15 μg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure shows the specificity of the antibody for the ER alpha isoform.
ChIP assays was performed using MCF7 cells treated with the ER agonist estradiol for 3 hours prior to harvesting, ER alpha (5G7) Monoclonal Antibody (bsm-53007M), and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 μg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene used as a negative control. Figure shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha.

Catalog #: bsm-53007M

Applications: WB, IHC, ChIP

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-52240R

Applications: WB, FCM, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-52241R

Applications: WB, FCM, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-52244R

Applications: WB, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-52245R

Applications: WB, FCM, IF(ICC), IHC

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: Unconjugated

Catalog #: bsm-52247R

Applications: WB, IHC

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: Unconjugated

Catalog #: bsm-52248R

Applications: WB, IF(ICC), IHC

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: Unconjugated

Catalog #: bsm-52249R

Applications: WB, FCM, IF(ICC), IP, IHC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #: bsm-52250R

Applications: WB, FCM, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-52251R

Applications: WB, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-52252R

Applications: WB, FCM, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-52253R

Applications: WB, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-52254R

Applications: WB, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-52255R

Applications: WB, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-52256R

Applications: WB, IHC

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-52257R

Applications: WB, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-52260R

Applications: WB, FCM, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-52261R

Applications: WB, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-52262R

Applications: WB, FCM, IF(ICC), IHC

Reactivity: Human, Mouse, Rat, Chicken, Monkey, Zebrafish

Conjugate: Unconjugated

Catalog #: bsm-52263R

Applications: WB, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-52264R

Applications: WB, FCM, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-52265R

Applications: WB, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-52266R

Applications: WB, FCM, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-52271R

Applications: WB, FCM, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-52272R

Applications: WB, FCM, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-52273R

Applications: WB, IHC

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-52274R

Applications: WB, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-52275R

Applications: WB, FCM, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-52277R

Applications: WB, FCM, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-52281R

Applications: WB, FCM, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-52282R

Applications: WB, FCM, IF(ICC), IHC

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: Unconjugated

Catalog #: bsm-52283R

Applications: WB, FCM, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-52284R

Applications: WB, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-52285R

Applications: WB, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-52286R

Applications: WB, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-52289R

Applications: WB, FCM, IF(ICC), IHC

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #: bsm-52290R

Applications: WB, FCM, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-52291R

Applications: WB, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-52292R

Applications: WB, FCM, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-52297R

Applications: WB, IF(ICC), IHC

Reactivity: Human

Conjugate: Unconjugated

Catalog #: bsm-52300R

Applications: WB, IF(ICC), IHC

Reactivity: Others

Conjugate: Unconjugated

Catalog #: bsm-52301R

Applications: WB, FCM, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-52302R

Applications: WB, FCM, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-52304R

Applications: WB, FCM, IF(ICC), IHC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #: bsm-52306R

Applications: WB, FCM, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-52309R

Applications: WB, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bsm-52311R

Applications: WB, FCM, IF(ICC), IHC

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated