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Primary Antibodies



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Catalog #1: bs-0938R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-0938R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-0938R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #1: bs-0938R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy5

Catalog #1: bs-0938R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy7

Catalog #1: bs-0938R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: FITC

Catalog #1: bs-0938R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: PE

Formalin-fixed and paraffin embedded human glioma tissue labeled with Anti-WIG-1\/PAG608 Polyclonal Antibody, Unconjugated (bs-0942R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human esophageal carcinoma labeled with Anti-WIG-1\/PAG608 Polyclonal Antibody, Unconjugated (bs-0942R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Lane 1: Human 293T cell lysates; Lane 2: Human HUVEC cell lysates; Lane 3: Human U-87MG cell lysates; Lane 4: Human U-2OS cell lysates probed with PAG608 Polyclonal Antibody, Unconjugated (bs-0942R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0942R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-0942R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-0942R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

HepG2 lysates probed with Serum albumin Polyclonal Antibody, Unconjugated (bs-0945R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Human Serum; Lane 2: Mouse Serum; Lane 3: Rat Serum; Lane 4: Goat Serum; Lane 5: Rat Liver lysates probed with Serum albumin Polyclonal Antibody, Unconjugated (bs-0945R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Albumin) polyclonal Antibody, Unconjugated (bs-0945R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Mouse Serum; Lane 2: Rat Serum; Lane 3: Human Serum; Lane 4: Goat Serum probed with Human Serum albumin Polyclonal Antibody, Unconjugated (bs-0945R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0945R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: Unconjugated

Catalog #1: bs-0942R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #1: bs-0942R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy5

Catalog #1: bs-0942R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy7

Catalog #1: bs-0942R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: FITC

Catalog #1: bs-0945R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Goat

Conjugate: Biotin

Catalog #1: bs-0945R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Goat

Conjugate: HRP

Catalog #1: bs-0945R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: Cy5

Catalog #1: bs-0945R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: Cy7

Catalog #1: bs-0945R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: Cy3

Catalog #1: bs-0945R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: FITC

Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Collagen III Polyclonal Antibody, Unconjugated (bs-0948R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Hela lysates probed with Collagen III Polyclonal Antibody, Unconjugated (bs-0948R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
25 ug total protein per lane of various lysates (see on figure) probed with Collagen III polyclonal antibody, unconjugated (bs-0948R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed RD (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Collagen III) polyclonal Antibody, unconjugated (bs-0948R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Catalog #1: bs-0948r

Applications: WB, Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0948R-Biotin

Applications: WB

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-0948R-HRP

Applications: WB

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-0948R-Cy7

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-0948R-Cy3

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-0948R-Cy5

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-0948R-FITC

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-0953R

Applications: ELISA, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-0952R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0953R-Cy5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy5

Catalog #1: bs-0953R-Biotin

Applications: ELISA, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-0953R-HRP

Applications: ELISA, IHC-F

Reactivity:

Conjugate: HRP

Catalog #1: bs-0953R-Cy3

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy3

Catalog #1: bs-0953R-Cy7

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy7

Catalog #1: bs-0955R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-0955R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-0953R-FITC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: FITC

Catalog #1: bs-0955R-Cy5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy5

Catalog #1: bs-0955R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Catalog #1: bs-0955R-Cy3

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy3

Catalog #1: bs-0955R-FITC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: FITC

Catalog #1: bs-0955R-Cy7

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy7

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PAG608 Polyclonal Antibody, Unconjugated (bs-0957R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Human 293T cell lysates; Lane 2: Human HUVEC cell lysates; Lane 3: Human U-87MG cell lysates; Lane 4: Human U-2OS cell lysates probed with PAG608 Polyclonal Antibody, Unconjugated (bs-0957R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0957R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Image kindly submitted by Jennifer DeMuro as part of the Free Sample Program. Mouse brain lysates probed with Rabbit Anti-Leptin receptor Polyclonal Antibody (bs-0961R).
K562 cells probed with Leptin receptor Polyclonal Antibody, Unconjugated (bs-0961R) at 3ug for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Leptin receptor Polyclonal Antibody, Unconjugated (bs-0961R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Lane 1: Mouse Liver; Probed with Leptin Receptor Polyclonal Antibody, Unconjugated (bs-0961R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bs-0961R

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0961R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-0961R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-0961R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7