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Primary Antibodies

Primary Antibodies

Our Primary Antibodies are research-use antibodies designed to support protein detection and target analysis across a wide range of applications and species. Our collection includes recombinant antibodies, monoclonal antibodies, and polyclonal antibodies for use in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), flow cytometry (FC), ELISA, and other common applications.

These primary antibodies are available for targets involved in cancer research, immunology, neuroscience, cell biology, metabolism, apoptosis, signaling pathways, and disease-related research. Bioss offers primary antibodies guaranteed for use in human, mouse, rat, and other species to help researchers choose the right antibody format for their experimental model and detection method.



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Catalog #1: bs-2010R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Cy5

Catalog #1: bs-2010R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Cy3

Formalin-fixed and paraffin embedded rat brain labeled with Anti- NIT2 Polyclonal Antibody, Unconjugated (bs-2012R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse embryo tissue labeled Anti-NIT2 Polyclonal Antibody, Unconjugated (bs-2012R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
A431 cell lysates probed with NIT2 Polyclonal Antibody, Unconjugated (bs-2012R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2012R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2010R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: FITC

Catalog #1: bs-2014R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-2010R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Cy7

Catalog #1: bs-2014R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-2014R-Cy7

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy7

Catalog #1: bs-2014R-Cy3

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy3

Catalog #1: bs-2014R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Catalog #1: bs-2014R-Cy5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy5

Catalog #1: bs-2014R-FITC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: FITC

Catalog #1: bs-2016R

Applications: WB, ELISA, FCM, IHC-P, IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-2016R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bs-2016R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human, Rat

Conjugate: HRP

Catalog #1: bs-2016R-Cy3

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy3

Catalog #1: bs-2016R-Cy5

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy5

Catalog #1: bs-2016R-FITC

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Rat

Conjugate: FITC

Catalog #1: bs-2017R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Plant

Conjugate: Biotin

Catalog #1: bs-2016R-Cy7

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy7

Oat lysates probed with 14-3-3 family protein Polyclonal Antibody, Unconjugated (bs-2017R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Wheat lysates probed with 14-3-3 family protein Polyclonal Antibody, Unconjugated (bs-2017R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-2017r

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Plant

Conjugate: Unconjugated

Catalog #1: bs-2017R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Plant

Conjugate: HRP

Catalog #1: bs-2018R

Applications: ELISA, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Plant

Conjugate: Unconjugated

Catalog #1: bs-2017R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Plant

Conjugate: Cy3

Catalog #1: bs-2017R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Plant

Conjugate: Cy7

Catalog #1: bs-2017R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Plant

Conjugate: FITC

Catalog #1: bs-2017R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Plant

Conjugate: Cy5

Catalog #1: bs-2018R-HRP

Applications: ELISA, IHC-F

Reactivity: Plant

Conjugate: HRP

Catalog #1: bs-2018R-Cy3

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Plant

Conjugate: Cy3

Catalog #1: bs-2018R-Cy5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Plant

Conjugate: Cy5

Catalog #1: bs-2018R-FITC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Plant

Conjugate: FITC

Catalog #1: bs-2018R-Cy7

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Plant

Conjugate: Cy7

Catalog #1: bs-2029R

Applications: ELISA

Reactivity: Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-PMP2 Polyclonal Antibody, Unconjugated (bs-2032R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat brain labeled with Anti-PMP2 Polyclonal Antibody, Unconjugated(bs-2032R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.
SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (PMP2) polyclonal Antibody, Unconjugated (bs-2032R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
SH-SY5Y cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with PMP2 Antibody(bs-2032R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-2032R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2032R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-2032R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-2032R-Cy3

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-2032R-Cy7

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-2032R-FITC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Antigen: bs-2033P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat Anti-Rabbit IgG (bs-0295G-HRP) at 1: 5000; \\nTMB staining; Read the data in MicroplateReader by 450nm.
Lane 1: E.coli lysates; Lane 2: E.coli lysates; Probed with E.coli DH-5 Alpha Polyclonal Antibody, Unconjugated (bs-2033R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2033R

Applications: WB, ELISA, IF(IHC-P)

Reactivity: Bacteria, Others

Conjugate: Unconjugated

Catalog #1: bs-2033R-Biotin

Applications: WB, ELISA

Reactivity: Bacteria, Others

Conjugate: Biotin

Catalog #1: bs-2033R-HRP

Applications: WB, ELISA

Reactivity: Bacteria, Others

Conjugate: HRP

Formalin-fixed and paraffin embedded rat pancreatic cancer labeled with Anti-CRIM1 Polyclonal Antibody, Unconjugated (bs-2034R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse kidney labeled with Anti-CRIM1 Polyclonal Antibody, Unconjugated (bs-2034R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Testis tissue lysates; Lane 2: Rat Testis tissue lysates; Lane 3: Human Jurkat cell lysates probed with CRIM1 Polyclonal Antibody, Unconjugated (bs-2034R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2034R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti--LIS1 Polyclonal Antibody, Unconjugated (bs-2035R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Mouse hippocampus lysates probed with LIS1Polyclonal Antibody, Unconjugated (bs-2035R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2035R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2035R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: HRP

Catalog #1: bs-2035R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: FITC

Catalog #1: bs-2035R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-2035R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Cy5